Fabrication of electrochemical biosensor composed of multi-functional DNA/rhodium nanoplate heterolayer for thyroxine detection in clinical sample

Park, Sun Yong; Kim, Jinmyeong; Yim, Gyeonghye; Jang, Hongje; Lee, Yeon-Ju; Kim, Soo Min; Park, Chulhwan; Lee, Taek

doi:10.1016/j.colsurfb.2020.111240

Cited by 31 publications

(8 citation statements)

References 31 publications

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“…For electrochemical measurements, polydimethylsiloxane (PDMS) was attached to the electrode and used as a chamber. PDMS was prepared by reacting sylgard 184A (DOW, Midland, MI, USA) and sylgard 184B (DOW, Midland, MI, USA) in a 10:1 ratio and drying in an oven at 70 °C for 1.5 h. Figure 2 shows the electrode drawing and preparation process [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Fabrication of an Electrochemical Aptasensor Composed of Multifunctional DNA Three-Way Junction on Au Microgap Electrode for Interferon Gamma Detection in Human Serum

et al. 2021

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Interferon gamma (IFN-γ) is an important cytokine with antiviral, antibacterial, and immunosuppressive properties. It has been used as a biomarker for the early detection of several diseases, including cancer, human immunodeficiency virus (HIV), tuberculosis, and paratuberculosis. In this study, we developed an electrochemical biosensor composed of multifunctional DNA 3WJ to detect IFN-γ level with high sensitivity. Each multifunctional triple-stranded aptamer (MF-3WJ) was designed to have an IFN-γ aptamer sequence, anchoring region (thiol group), and 4C–C (cytosine–cytosine) mismatch sequence (signal generation), which could introduce silver ions. To generate the electrochemical signal, four Ag+ ions were intercalated (3wj b-3wj c) in the 4C–C mismatch sequence. MF-3WJ was assembled through the annealing step, and the assembly of MF-3WJ was confirmed by 8% tris–boric–EDTA native polyacrylamide gel electrophoresis. The Au microgap electrode was manufactured to load sample volumes of 5 µL. The reliability of electrochemical biosensor measurement was established by enabling the measurement of seven samples from one Au microgap electrode. MF-3WJ was immobilized on the Au microgap electrode. Then, cyclic voltammetry and electrochemical impedance spectroscopy were performed to confirm the electrochemical properties of MF-3WJ. To test the electrochemical biosensor’s ability to detect IFN-γ, the limit of detection (LOD) and selectivity tests were performed by square wave voltammetry. A linear region was observed in the concentration range of 1 pg/mL–10 ng/mL of IFN-γ. The LOD of the fabricated electrochemical biosensor was 0.67 pg/mL. In addition, for the clinical test, the LOD test was carried out for IFN-γ diluted in 10% human serum samples in the concentration range of 1 pg/mL–10 ng/mL, and the LOD was obtained at 0.42 pg/mL.

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Section: Methodsmentioning

confidence: 99%

Fabrication of an Electrochemical Aptasensor Composed of Multifunctional DNA Three-Way Junction on Au Microgap Electrode for Interferon Gamma Detection in Human Serum

et al. 2021

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“…Electrochemical impedance spectroscopy (EIS) is a powerful tool used for probing the electrode surfaces. Impedance indicates the degree to which the electrical flow is interrupted for alternating current, and the electrode surface is analyzed using the characteristics of amplitude change and phase change according to the frequency [ 83 , 84 , 85 , 86 , 87 ]. Figure 2 shows a schematic of the electrochemical measurements.…”

Section: Electrochemical-based Detectionmentioning

confidence: 99%

Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Park

et al. 2023

Nanomaterials

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Zika virus (ZIKV) and dengue virus (DENV) are highly contagious and lethal mosquito-borne viruses. Global warming is steadily increasing the probability of ZIKV and DENV infection, and accurate diagnosis is required to control viral infections worldwide. Recently, research on biosensors for the accurate diagnosis of ZIKV and DENV has been actively conducted. Moreover, biosensor research using DNA nanotechnology is also increasing, and has many advantages compared to the existing diagnostic methods, such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). As a bioreceptor, DNA can easily introduce a functional group at the 5′ or 3′ end, and can also be used as a folded structure, such as a DNA aptamer and DNAzyme. Instead of using ZIKV and DENV antibodies, a bioreceptor that specifically binds to viral proteins or nucleic acids has been fabricated and introduced using DNA nanotechnology. Technologies for detecting ZIKV and DENV can be broadly divided into electrochemical, electrical, and optical. In this review, advances in DNA-nanotechnology-based ZIKV and DENV detection biosensors are discussed.

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“…Because of their unique properties, nanomaterials are applied in various fields [99][100][101]. In particular, with respect to biosensors, various nanoparticles can be used to improve the efficiency and sensitivity of both transducers and receptors [102]. This is because of the high area-to-volume ratio [49], optical properties (such as luminescence, fluorescence, refractive index, light scattering [103]), and high electrical activity [104] of nanoparticles.…”

Section: Nanomaterialsmentioning

confidence: 99%

Recent Advances in Aptasensor for Cytokine Detection: A Review

Kim

Noh

Park

et al. 2021

Sensors

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Cytokines are proteins secreted by immune cells. They promote cell signal transduction and are involved in cell replication, death, and recovery. Cytokines are immune modulators, but their excessive secretion causes uncontrolled inflammation that attacks normal cells. Considering the properties of cytokines, monitoring the secretion of cytokines in vivo is of great value for medical and biological research. In this review, we offer a report on recent studies for cytokine detection, especially studies on aptasensors using aptamers. Aptamers are single strand nucleic acids that form a stable three-dimensional structure and have been receiving attention due to various characteristics such as simple production methods, low molecular weight, and ease of modification while performing a physiological role similar to antibodies.

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Fabrication of electrochemical biosensor composed of multi-functional DNA/rhodium nanoplate heterolayer for thyroxine detection in clinical sample

Cited by 31 publications

References 31 publications

Fabrication of an Electrochemical Aptasensor Composed of Multifunctional DNA Three-Way Junction on Au Microgap Electrode for Interferon Gamma Detection in Human Serum

Fabrication of an Electrochemical Aptasensor Composed of Multifunctional DNA Three-Way Junction on Au Microgap Electrode for Interferon Gamma Detection in Human Serum

Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Recent Advances in Aptasensor for Cytokine Detection: A Review

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