Fabrication of electropsun PLGA and small intestine submucosa-blended nanofibrous membranes and their biocompatibility for wound healing

Kim, Ki-Hoon; Lee, Jong Yoon; Kim, Hyun; Shin, Jaiho; Shin, Yongwon; Yoo, Young-Tae; Kim, Hwi-Yool

doi:10.1007/s12221-017-6936-y

Cited by 6 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, Kim et al . used SIS and PLGA to prepare hybrid nanofiber membranes by electrospinning to improve mechanical properties and compatibility [ 103 ].…”

Section: Surface Modification and Practical Application Of Sismentioning

confidence: 99%

Surface modification of small intestine submucosa in tissue engineering

Pan

Qin

et al. 2020

Regenerative Biomaterials

View full text Add to dashboard Cite

With the development of tissue engineering, the required biomaterials need to have the ability to promote cell adhesion and proliferation in vitro and in vivo. Especially, surface modification of the scaffold material has a great influence on biocompatibility and functionality of materials. The small intestine submucosa (SIS) is an extracellular matrix isolated from the submucosal layer of porcine jejunum, which has good tissue mechanical properties and regenerative activity, and is suitable for cell adhesion, proliferation and differentiation. In recent years, SIS is widely used in different areas of tissue reconstruction, such as blood vessels, bone, cartilage, bladder and ureter, etc. This paper discusses the main methods for surface modification of SIS to improve and optimize the performance of SIS bioscaffolds, including functional group bonding, protein adsorption, mineral coating, topography and formatting modification and drug combination. In addition, the reasonable combination of these methods also offers great improvement on SIS surface modification. This article makes a shallow review of the surface modification of SIS and its application in tissue engineering.

show abstract

“…In addition, Kim et al . used SIS and PLGA to prepare hybrid nanofiber membranes by electrospinning to improve mechanical properties and compatibility [ 103 ].…”

Section: Surface Modification and Practical Application Of Sismentioning

confidence: 99%

Surface modification of small intestine submucosa in tissue engineering

Pan

Qin

et al. 2020

Regenerative Biomaterials

View full text Add to dashboard Cite

show abstract

“…When the orientation of the nanofibers was the same, the addition of SIS significantly decreased the diameters ( p < 0.05). This is assumed to result from the increased electrical conductivity due to the incorporation of SIS into PLGA [ 25 , 26 ]. However, under the same NFM composition, the diameter of the aligned nanofibers decreased significantly compared to that of the random nanofibers ( p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

“…Random electrospun NFMs were obtained from aluminum foil fixed on a flat metal collector [ 26 ]. A rotating metal collector was used to fabricate uniaxially aligned nanofibers.…”

Section: Methodsmentioning

confidence: 99%

“…Several studies have used SIS as a blending material for hybridization with PCL [ 24 , 25 ]. In our previous study, we developed a novel scaffold of electrospun PLGA and SIS blended nanofibrous membranes and identified their potential for wound healing in an in vitro study [ 26 ]. However, these novel scaffolds were in non-woven web forms, and the mechanical strength of random nanofibers was generally poor [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Potential of Aligned Electrospun PLGA/SIS Blended Nanofibrous Membrane for Tendon Tissue Engineering

Kim

Hee

et al. 2023

Polymers

Self Cite

View full text Add to dashboard Cite

Tendons are responsible for transmitting mechanical forces from muscles to bones for body locomotion and joint stability. However, tendons are frequently damaged with high mechanical forces. Various methods have been utilized for repairing damaged tendons, including sutures, soft tissue anchors, and biological grafts. However, tendons experience a higher rate of retear post-surgery due to their low cellularity and vascularity. Surgically sutured tendons are vulnerable to reinjury due to their inferior functionality when compared with native tendons. Surgical treatment using biological grafts also has complications such as joint stiffness, re-rupture, and donor-site morbidity. Therefore, current research is focused on developing novel materials that can facilitate the regeneration of tendons with histological and mechanical characteristics similar to those of intact tendons. With respect to the complications in association with the surgical treatment of tendon injuries, electrospinning may be an alternative for tendon tissue engineering. Electrospinning is an effective method for fabrication of polymeric fibers with diameters ranging from nanometers to micrometers. Thus, this method produces nanofibrous membranes with an extremely high surface area-to-volume ratio, which is similar to the extracellular matrix structure, making them suitable candidates for application in tissue engineering. Moreover, it is possible to fabricate nanofibers with specific orientations that are similar to those of the native tendon tissue using an adequate collector. To increase the hydrophilicity of the electrospun nanofibers, natural polymers in addition to synthetic polymers are used concurrently. Therefore, in this study, aligned nanofibers composed of poly-d,l-lactide-co-glycolide (PLGA) and small intestine submucosa (SIS) were fabricated using electrospinning with rotating mandrel. The diameter of aligned PLGA/SIS nanofibers was 568.44 ± 135.594 nm, which closely resembles that of native collagen fibrils. Compared to the results of the control group, the mechanical strength exhibited by the aligned nanofibers was anisotropic in terms of break strain, ultimate tensile strength, and elastic modulus. Elongated cellular behavior was observed in the aligned PLGA/SIS nanofibers using confocal laser scanning microscopy, indicating that the aligned nanofibers were highly effective with regard to tendon tissue engineering. In conclusion, considering its mechanical properties and cellular behavior, aligned PLGA/SIS is a promising candidate for tendon tissue engineering.

show abstract

“…Wang et al successfully prepared a proangiogenic dSIS gel, which successfully promoted neovascularization as compared to the collagen type I gel in vivo [12]. Kim et al fabricated the blended nanofibrous membrane of poly(lactic-co-glycolic acid) and SIS for improved mechanical properties and biocompatibility using the single-stream electrospinning method [13]. Owing to its high biocompatibility, wound healing ability, and tissue regeneration potential, dSIS is approved by the US Food and Drug Administration for a number of clinical applications and there is a commercially available SIS-derived product, Oasis®, for wound healing application [14].…”

Section: Introductionmentioning

confidence: 99%

Cryogenic free-form extrusion bioprinting of decellularized small intestinal submucosa for potential applications in skin tissue engineering

Shi

Ullah

et al. 2019

Biofabrication

View full text Add to dashboard Cite

A novel strategy of cryogenic 3D bioprinting assisted by free-from extrusion printing has been developed and applied to printing of a decellularized small intestinal submucosa (dSIS) slurry. The rheological properties, including kinetic viscosity, storage modulus (G′), and loss modulus (G″), were appropriate for free-from extrusion printing of dSIS slurry. Three different groups of scaffolds, including P500, P600, and P700, with filament distances of 500, 600, and 700 μm, respectively were fabricated at a 5 mm s−1 working velocity of the platform (Vxy) and 25 kPa air pressure of the dispensing system (P) at −20 °C. The fabricated scaffolds were crosslinked via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) which resulted in a polyporous microstructure. The variations in the filament diameter and pore size were evaluated in the initial frozen state after printing, the lyophilized state, and after immersion in a PBS solution. The Young’s modulus of the P500, P600, and P700 scaffolds was measured in wet and dry states for EDC-crosslinked scaffolds. The cell experiment results showed improved cell adhesion, viability, and proliferation both on the surface and within the scaffold, indicating the biocompatibility and suitability of the scaffold for 3D cell models. Further, gene and protein expression of normal skin fibroblasts on dSIS scaffolds demonstrated their ability to promote the production of some extracellular matrix proteins (i.e. collagen I, collagen III, and fibronectin) in vitro. Overall, this study presents a new potential strategy, by combining cryogenic 3D bioprinting with decellularized extracellular matrix materials, to manufacture ideal scaffolds for skin tissue engineering applications.

show abstract

Fabrication of electropsun PLGA and small intestine submucosa-blended nanofibrous membranes and their biocompatibility for wound healing

Cited by 6 publications

References 35 publications

Surface modification of small intestine submucosa in tissue engineering

Surface modification of small intestine submucosa in tissue engineering

Potential of Aligned Electrospun PLGA/SIS Blended Nanofibrous Membrane for Tendon Tissue Engineering

Cryogenic free-form extrusion bioprinting of decellularized small intestinal submucosa for potential applications in skin tissue engineering

Contact Info

Product

Resources

About