Fabrication of engineered heart tissue grafts from alginate/collagen barium composite microbeads

Bai, Xiuping; Zheng, Huifang; Fang, Rui; Wang, T R; Hou, Xiaolu; Li, Y; Chen, Daniel; Tian, Weiming

doi:10.1088/1748-6041/6/4/045002

Cited by 56 publications

(25 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5 E-G) [94]. Alginate has also been combined with collagen using an electrostatic dispersion method to create cell-containing beads 200-3000 μm in diameter for cardiac regeneration [95]. …”

Section: 0 - Isolation and Reconstitution Of Collagen Into Hydrogelmentioning

confidence: 99%

Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales

2014

View full text Add to dashboard Cite

Collagen type I is a widely used natural biomaterial that has found utility in a variety of biological and medical applications. Its well characterized structure and role as an extracellular matrix protein make it a highly relevant material for controlling cell function and mimicking tissue properties. Collagen type I is abundant in a number of tissues, and can be isolated as a purified protein. This review focuses on hydrogel biomaterials made by reconstituting collagen type I from a solubilized form, with an emphasis on in vitro studies in which collagen structure can be controlled. The hierarchical structure of collagen from the nanoscale to the macroscale is described, with an emphasis on how structure is related to function across scales. Methods of reconstituting collagen into hydrogel materials are presented, including molding of macroscopic constructs, creation of microscale modules, and electrospinning of nanoscale fibers. The modification of collagen biomaterials to achieve desired structures and functions is also addressed, with particular emphasis on mechanical control of collagen structure, creation of collagen composite materials, and crosslinking of collagenous matrices. Biomaterials scientists have made remarkable progress in rationally designing collagen-based biomaterials and in applying them to both the study of biology and for therapeutic benefit. This broad review illustrates recent examples of techniques used to control collagen structure, and to thereby direct its biological and mechanical functions.

show abstract

Section: 0 - Isolation and Reconstitution Of Collagen Into Hydrogelmentioning

confidence: 99%

Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales

2014

View full text Add to dashboard Cite

show abstract

“…38,39 However, in these studies, liquid alginate was used that diffuses through the vessel wall. 38 Although eMSC cannot leave the vessel lumen, they seem to provide structural support to the myocardium.…”

Section: Encapsulated Mscs Preserve LV Function After Amimentioning

confidence: 99%

Intracoronary Infusion of Encapsulated Glucagon-Like Peptide-1–Eluting Mesenchymal Stem Cells Preserves Left Ventricular Function in a Porcine Model of Acute Myocardial Infarction

Jong

Hout

Houtgraaf

et al. 2014

Circ: Cardiovascular Interventions

View full text Add to dashboard Cite

R egenerative stem cell therapy to promote cardiac repair has been a target of interest in the past 10 years to prevent heart failure after an acute myocardial infarction (AMI). 1,2 Various different stem cells have been investigated for their ability to repair the heart.3 Mesenchymal stem cells (MSCs) seem to be a potent candidate to date. The mechanism of action of MSC is primarily based on the release of paracrine factors to the myocardium. 4 However, retention and survival of stem cells in the myocardium after intracoronary infusion remains an issue in cell-based therapy, because only a limited number of surviving stem cells remain in the myocardium, thereby limiting the potential benefit of the therapy. [5][6][7] CellBeads, which consist of alginate-encapsulated MSCs (eMSCs; BTG International Germany GmbH, Alzenau, Germany), were developed to improve survival of cells in the myocardium, thereby elongating the release of cardioprotective proteins into the infarcted myocardium. These eMSCs secrete endogenous paracrine factors that include vascular endothelial growth factor, monocyte chemotactic protein-1, interleukin (IL)-6, IL-8, glial-derived neurotrophic factor, and neurotrophin-3 and are genetically modified to produce glucagon-like peptide-1 (GLP-1) fusion protein, which comprises 2 GLP-1 molecules bound by an intervening peptide, extending its half-life in vivo. [8][9][10][11][12] Among its beneficial effectsBackground-Engraftment and survival of stem cells in the infarcted myocardium remain problematic in cell-based therapy for cardiovascular disease. To overcome these issues, encapsulated mesenchymal stem cells (eMSCs) were developed that were transfected to produce glucagon-like peptide-1, an incretin hormone with known cardioprotective effects, alongside MSC endogenous paracrine factors. This study was designed to investigate the efficacy of different doses of intracoronary infusion of eMSC in a porcine model of acute myocardial infarction (AMI). Methods and Results-One hundred pigs were subjected to a moderate AMI (posterolateral AMI; n=50) or a severe AMI (anterior AMI; n=50), whereupon surviving animals (n=36 moderate, n=33 severe) were randomized to receive either intracoronary infusion of 3 incremental doses of eMSC or Ringers' lactate control. Cardiac function was assessed using invasive hemodynamics, echocardiography, and histological analysis. A trend was observed in the moderate AMI model, whereas in the severe AMI model, left ventricular ejection fraction improved by +9.3% (P=0.004) in the best responding eMSC group, because of a preservation of left ventricular end-systolic volume. Arteriolar density increased 3-fold in the infarct area (8.4±0.9/mm 2 in controls versus 22.2±2.6/mm 2 in eMSC group; P<0.001). Although not statistically significant, capillary density was 30% higher in the border zone (908.1±99.7/mm 2 in control versus 1209.0±64.6/mm 2 in eMSC group; P=ns). Conclusions-eMSCs enable sustained local delivery of cardioprotective proteins to the heart, thereby enhancing angio...

show abstract

“…Therefore, the mechanical properties of the material must be understood in order to describe and control the stress fields which the cells experience [10,11], as well as to control and characterize material performance. Alginate beads can be produced with a broad range of sized (1-1,000 lm) and find applications in drug delivery, imunoprotection of cells for transplantation and cell based therapies and as scafold material for tissue engineering [7,[13][14][15]. For hydrogel based composite materials, where for example polymer network is reinforced by a presence of mineral component, their mechanical properties will not only depend on the choice of materials and mineral content, but also on organization of components on different length scales [16].…”

Section: Introductionmentioning

confidence: 99%

Viscoelastic properties of mineralized alginate hydrogel beads

Olderøy

Xie

Andreassen

et al. 2012

J Mater Sci: Mater Med

View full text Add to dashboard Cite

Alginate hydrogels have applications in biomedicine, ranging from delivery of cells and growth factors to wound management aids. However, they are mechanically soft and have shown little potential for the use in bone tissue engineering. Here, the viscoelastic properties of alginate hydrogel beads mineralized with calcium phosphate, both by a counter-diffusion (CD) and an enzymatic approach, are characterized by a micro-manipulation technique and mathematical modeling. Fabricated hydrogel materials have low mineral content (below 3 % of the total hydrogel mass, which corresponds to mineral content of up to 60 % of the dry mass) and low dry mass content (<5 %). For all samples compression and hold (relaxation after compression) data was collected and analyzed. The apparent Young's modulus of the mineralized beads was estimated by the Hertz model (compression data) and was shown to increase up to threefold upon mineralization. The enzymatically mineralized beads showed higher apparent Young's modulus compared to the ones mineralized by CD, even though the mineral content of the former was lower. Full compression-relaxation force-time profiles were analyzed using viscoelastic model. From this analysis, infinite and instantaneous Young's moduli were determined. Similarly, enzymatic mineralized beads, showed higher instantaneous and infinite Young's modulus, even if the degree of mineralization is lower then that achieved for CD method. This leads to the conclusion that both the degree of mineralization and the spatial distribution of mineral are important for the mechanical performance of the composite beads, which is in analogy to highly structured mineralized tissues found in many organisms.

show abstract

Fabrication of engineered heart tissue grafts from alginate/collagen barium composite microbeads

Cited by 56 publications

References 47 publications

Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales

Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales

Intracoronary Infusion of Encapsulated Glucagon-Like Peptide-1–Eluting Mesenchymal Stem Cells Preserves Left Ventricular Function in a Porcine Model of Acute Myocardial Infarction

Viscoelastic properties of mineralized alginate hydrogel beads

Contact Info

Product

Resources

About