Fabrication of high specificity hollow mesoporous silica nanoparticles assisted by Eudragit for targeted drug delivery

She, Xiaodong; Chen, Lijue; Velleman, Leonora; Li, Chengpeng; Zhu, Haijin; He, Canzhong; Wang, Tao; Shigdar, Sarah; Duan, Wei; Kong, Lingxue

doi:10.1016/j.jcis.2014.12.053

Cited by 62 publications

(40 citation statements)

References 73 publications

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“…Ultimately, the cells were stained with 1 µg/mL Hoechst 33342 for 20 min and 1 µg/mL rhodamine phalloidin for 30 min. Meanwhile, FITC-LCP at different concentrations (20,25, and 50 µg/mL) was cultured for 1 h according to the aforementioned methods. The samples were observed by CLSM (Leica).…”

Section: Flow Cytometry Assaysmentioning

confidence: 99%

Application of a lipid-coated hollow calcium phosphate nanoparticle in synergistic co-delivery of doxorubicin and paclitaxel for the treatment of human lung cancer A549 cells

Hao

et al. 2017

IJN

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In this study, we developed a lipid-coated hollow calcium phosphate (LCP) nanoparticle for the combined application of two chemotherapeutic drugs to human lung cancer A549 cells. Hydrophilic doxorubicin (DOX) was incorporated into the hollow structure of hollow calcium phosphate (HCP), and a lipid bilayer containing hydrophobic paclitaxel (PTX) was subsequently coated on the surface of HCP. The study on combinational effects demonstrated that the combination of DOX and PTX at a mass ratio of 12:1 showed a synergistic effect against A549 cells. The particle size, zeta potential, and encapsulation efficiency were measured to obtain optimal values: particle size was 335.0 3.2 nm, zeta potential −41.1 mV, and encapsulation efficiency 80.40%±2.24%. An in vitro release study indicated that LCP produced a sustained drug release. A549 cells had a better uptake of LCP with good biocompatibility. Furthermore, in vitro cytotoxicity experiment, apoptosis analysis, in vivo anti-tumor efficacy and protein expression analysis of Bax, Bcl-2, and Caspase-3 demonstrated that the co-delivery system based on LCP had significant synergistic anti-tumor activity. All conclusions suggested that LCP is a promising platform for co-delivery of multiple anti-tumor drugs.

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Section: Flow Cytometry Assaysmentioning

confidence: 99%

Application of a lipid-coated hollow calcium phosphate nanoparticle in synergistic co-delivery of doxorubicin and paclitaxel for the treatment of human lung cancer A549 cells

Hao

et al. 2017

IJN

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“…47 Briefly, an acetone solution of Eudragit S-100 (4 mg/mL) was added dropwise to deionised water (10 mL) under stirring and the solution was left to proceed for 5 h under agitation at room temperature to obtain Eudragit particles. 47 Briefly, an acetone solution of Eudragit S-100 (4 mg/mL) was added dropwise to deionised water (10 mL) under stirring and the solution was left to proceed for 5 h under agitation at room temperature to obtain Eudragit particles.…”

Section: Preparation Of Hmsns For Drug Loading and Egfr Targetingmentioning

confidence: 99%

“…To overcome ADR in colorectal cancer, more robust nanocarriers with Paper Nanoscale 2 | J. 47 Epidermal Growth Factor (EGF) was grafted on the surface of HMSNs to target the EGFR which are overexpressed on colorectal cancer cells. Since the Mesoporous Silica Nanoparticles (MSNs) was first introduced as a drug nanocarrier in 2000, 20 studies on MSNs as a drug delivery system have received great attention.…”

Section: Introductionmentioning

confidence: 99%

Overcoming acquired drug resistance in colorectal cancer cells by targeted delivery of 5-FU with EGF grafted hollow mesoporous silica nanoparticles

et al. 2015

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Acquired drug resistance (ADR) can be developed in colorectal cancer cells after 5-fluorouracil (5-FU) treatment and diminish the effectiveness of chemotherapy. In this work, acquired 5-FU resistance in the colorectal cancer cell line SW480 was obtained with the up-regulation of dihydropyrimidine dehydrogenase (DPYD) gene expression which can convert 5-FU to its inactive metabolite. To overcome ADR in colorectal cancer, hollow mesoporous silica nanoparticles (HMSNs) grafted with epidermal growth factor (EGF) were used as nanocarriers to deliver 5-FU to colorectal cancer cells with acquired drug resistance. The effect and mechanism of 5-FU loaded EGF grafted HMSNs (EGF-HMSNs-5-FU) in overcoming acquired drug resistance in SW480/ADR cells were studied. The EGF-HMSNs were demonstrated to be specifically internalized in EGFR overexpressed SW480/ADR cells via a receptor-mediated endocytosis and can escape from endo-lysosomes. The EGF-HMSNs-5-FU exhibited much higher cytotoxicity on SW480/ADR cells than HMSNs-5-FU and free 5-FU while the plain HMSNs did not show significant cytotoxicity. The mechanism of EGF-HMSNs-5-FU in overcoming drug resistance in SW480/ADR cells could be attributed to the specific internalization of EGF-HMSNs-5-FU in EGFR overexpressed cells which can lead to high intracellular drug accumulation and cause cell death through S phase arrest.

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“…In particular, mesoporous silica, discovered by Mobil researchers [8], is seen as a potentially application in sensor because of its high surface, tunable pore size and volume, and well-defined surface properties for modification [9]. The studies on novel nanostructure particles, especially inorganic oxide (such as TiO 2 , SiO 2 and Fe 3 O 4 ) hollow microspheres [10], have been one of the most vibrant fields in materials science due to the thicker wall, large surface area and high chemical stability [11], which are excellent for sensor, catalysis, drug delivery, pH-responsive delivery carrier, bio-medical applications [12][13][14][15][16][17][18][19][20]. HMSMs and MSMs with mesoporous structures have attracted much attention due to their potential applications in sensor, confined-space catalysis, drug storage and release, etc.…”

Section: Introductionmentioning

confidence: 99%

Preparation, characterization and oxygen sensing properties of luminescent carbon dots assembled mesoporous silica microspheres

Wang

Zhang

Zhou

et al. 2016

Journal of Colloid and Interface Science

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Fabrication of high specificity hollow mesoporous silica nanoparticles assisted by Eudragit for targeted drug delivery

Cited by 62 publications

References 73 publications

Application of a lipid-coated hollow calcium phosphate nanoparticle in synergistic co-delivery of doxorubicin and paclitaxel for the treatment of human lung cancer A549 cells

Application of a lipid-coated hollow calcium phosphate nanoparticle in synergistic co-delivery of doxorubicin and paclitaxel for the treatment of human lung cancer A549 cells

Overcoming acquired drug resistance in colorectal cancer cells by targeted delivery of 5-FU with EGF grafted hollow mesoporous silica nanoparticles

Preparation, characterization and oxygen sensing properties of luminescent carbon dots assembled mesoporous silica microspheres

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