Fabrication of Magnetic Core–Shell CoFe2O4/Al2O3 Nanoparticles as Immobilized Metal Chelate Affinity Support for Protein Adsorption

Abstract

This study introduced the fabrication of core–shell magnetic CoFe2O4/Al2O3 nanocomposites as the immobilized affinity support for the detection of interleukin-6, as a model protein, in clinical immunoassay.

Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

“…53,219–222 Using cyanogen bromide supports, the enzyme is immobilized via several kinds of bond, and some of them are reversible, enabling enzyme release. 281–286 Enzyme immobilization via interaction of mainly His groups belonging to the enzyme (but also Cys, Tyr and others) with immobilized metal chelates may also have problems, 287–291 since the interaction may be broken at certain values of pH or if the medium contains particular compounds ( e.g. , His free amino acids or contained in peptides if using immobilized proteases to hydrolyze proteins).…”

Enhanced photocatalytic performance of CdFe2O4/Al2O3 nanocomposite for dye degradation

“…Aluminium oxide (Al 2 O 3 ) is an excellent ceramic material which is widely used as catalysts due to its high mechanical strength, hardness, good corrosion resistance at high temperatures, and outstanding dielectric properties (Tekiye et al 2016;Zhang et al 2012;Tang et al 2006;Rai et al 2017;Patra et al 2013). Iron oxide supported on alumina have been studied in photo-assisted fenton degradation of different textile dyes (Tekiye et al 2016).…”

ZnFe2O4 Containing Nanoparticles: Synthesis and Magnetic Properties

“…In recent times ferrite materials are used to avoid and remove radio frequency disturbances in audio systems [4], as polarized ferroelectric ceramic in acoustic elements of underwater sound transducers [15] and microwave absorbing materials [16], including ferrite containing radar absorbing colours for disguising military aircraft [17]. Various fabrication methods to prepare spinel ferrites nanocrystals have been reported, for example, the standard high temperature solid-state reaction technique [18], chemical co-precipitation method [19], sol-gel method [20], combustion reaction synthesis [21], hydrolysis [22], hydrothermal synthesis [23], salt melt technique [6], pyrolysis, different microwave synthesis methods [24], including microwave refluxing [25], microwave plasma [26] and the microwave-hydrothermal method [27], high energy ball milling technique [28], microemulsion method [29], sonochemical reaction [30], vapour deposition [31], precursor techniques [32], and plasma synthesis [33]. In our previous investigations [34], [35] we have studied synthesis of Ni and Co ferrite nanoparticles by the sol-gel selfpropagating combustion method and their magnetic properties.…”