Fabrication of magnetic dual-hydrophilic metal organic framework for highly efficient glycopeptide enrichment

Abstract

Highly selective glycopeptide enrichment is important before mass spectrometry analysis because of the ultra-low abundance of glycopeptides in the peptide mixtures. Herein, a UiO-66-NH 2 -based magnetic composite was prepared and used for the hydrophilic enrichment of glycopeptides. The composite was modified with phytic acid (PA) molecules by partially replacing 2aminoterephthalic acid ligands in UiO-66-NH 2 , with electrostatic interactions also promoting this modification process. Based on the hydrophilicity of both the PA molecules and the UiO-66-NH 2 skeleton, the resulting material, denoted as MUiO-66-NH 2 /PA, showed excellent dual hydrophilicity towards glycopeptide enrichment. Compared with pure UiO-66-NH 2 , the specific surface area and hydrophilicity of the prepared material were increased, and MUiO-66-NH 2 /PA exhibited good magnetic responsiveness to facilitate a convenient enrichment procedure. HRP and IgG were used as standard proteins to evaluate the glycopeptide enrichment properties, with 21 and 34 glycopeptides enriched from their tryptic digests. Furthermore, MUiO-66-NH 2 /PA showed outstanding sensitivity (1 fmol/μL) and selectivity (HRP/BSA = 1:1000), and achieved remarkable glycopeptide enrichment performance for practical human serum samples. Notably, MUiO-66-NH 2 /PA showed perfect reusability and stability, achieving enrichment performance after five cycles similar to that of the first use. This material can be used for glycopeptide enrichment to obtain further glycosylation information, providing the possibility for cancer treatment.

A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides

“…There are still 6 matched signals even in the case of 0.1 fmol mL À1 . The LOD was better than that of previously reported hydrophilic composites such as the tripeptide-functionalized magnetic MOF material (0.5 fmol mL À1 ), 18 the MG@Zn-MOF biocomposite (0.8 fmol mL À1 ), 26 and the magnetic dual-hydrophilic MOF material (1.0 fmol mL À1 ) 27 .…”

“…As shown in Table S3 (ESI†), the capture performance of the probe is in the top-rank and significantly superior to previously reported composites. 14,17,18,26–32…”

“…S8 (ESI †), it can be calculated that the loading capacity of the probe for glycopeptides is 200 mg g À1 , which is much higher than that previously reported in literature. 17,18,[27][28][29] Besides, the recovery of GO@Fe 3 O 4 @ MOF-303 for identifying glycopeptides from the IgG digests was evaluated using the isotope dimethyl labeling technique. The recovery yield (D/H) was calculated using the peak intensity ratio of heavy-tagged/light-tagged deglycosylated peptides.…”

“…As shown in Table S3 (ESI †), the capture performance of the probe is in the top-rank and significantly superior to previously reported composites. 14,17,18,[26][27][28][29][30][31][32] N-Glycoproteomic profiling of clinical samples Hepatocellular carcinoma (HCC) is one of the most malignant cancers with high morbidity and mortality. The high death rate of HCC is primarily related to the fact that most HCC cannot be diagnosed promptly and accurately.…”

A novel hydrophilic hydrogel with a 3D network structure for the highly efficient enrichment of N-glycopeptides

“…Currently, numerous glycopeptide enrichment strategies have been developed, including hydrophilic interaction liquid chromatography (HILIC), [14][15][16] boronic acid affinity method, [17][18][19] lectin affinity chromatography 20,21 and hydrazide chemistry. 22,23 Among them, HILIC with the features of good reproducibility, easy operation and great MS compatibility has been widely used for glycopeptide enrichment.…”