A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides

Wang, Xinmei; Li, Jie; Yan, Xia; Gao, Mingxia; Zhang, Xiangmin; Huang, Lihao

doi:10.1039/d1tb02827h

Cited by 18 publications

(7 citation statements)

References 43 publications

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“…5d). In comparison with the reported GO@Fe 3 O 4 @MOF-303 23 and magMOF@Au-maltose, 26 Fe 3 O 4 @COF@MOF-PS has better specific adsorption capacity.…”

Section: Resultsmentioning

confidence: 65%

In situ grown magnetic COF@MOF with a phosphoserine anchor for in-depth N-glycopeptide analysis in serum

Guo,

Hua,

Wang

et al. 2023

Analyst

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“…5d). In comparison with the reported GO@Fe 3 O 4 @MOF-303 23 and magMOF@Au-maltose, 26 Fe 3 O 4 @COF@MOF-PS has better specific adsorption capacity.…”

Section: Resultsmentioning

confidence: 65%

In situ grown magnetic COF@MOF with a phosphoserine anchor for in-depth N-glycopeptide analysis in serum

Guo,

Hua,

Wang

et al. 2023

Analyst

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“…12−14 Moreover, we have recently reported that serum glycoprotein profiles in HCC patients were significantly different from normal persons. 15 In this consideration, the large-scale characterization of serum N-glycans attaching to glycoproteins will have great potential for developing new diagnostic methods for early diagnosis during HCC development.…”

Section: ■ Introductionmentioning

confidence: 99%

“…N-Glycans are common glycan forms covalently attached to proteins, which can regulate various processes of cellular biology through modifications of glycoproteins. − It has been identified that the aberrant alterations of N-glycans such as multiple branching, sialylation, and fucosylation could be associated with the progression of various diseases. − Moreover, we have recently reported that serum glycoprotein profiles in HCC patients were significantly different from normal persons . In this consideration, the large-scale characterization of serum N-glycans attaching to glycoproteins will have great potential for developing new diagnostic methods for early diagnosis during HCC development.…”

Section: Introductionmentioning

confidence: 99%

Novel Three-Dimensional Hierarchical Porous Carbon Probe for the Discovery of N-Glycan Biomarkers and Early Hepatocellular Carcinoma Detection

Liu

Wang

et al. 2023

Anal. Chem.

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Due to the highly heterogeneous nature of hepatocellular carcinoma (HCC), the accurate diagnosis of HCC during the early phase of development is still a challenging task. Therefore, the further development of novel diagnostic methods by discovering new biomarkers is required to improve the rate of HCC diagnosis in the early phase. In this work, an oxygen-modified three-dimensional interconnected porous carbon probe is designed and fabricated to profile the differences of N-glycans in human serum from health controls (H) and patients with hepatic dysfunction (HD) and HCC for the discovery of new biomarkers with HCC development. Excitingly, we discovered that the expression levels of 12 serum N-glycans were gradually increased from H to patients with HD and eventually to patients with HCC. Moreover, two machine learning models established based on these 12 serum N-glycans reached a satisfactory accuracy for predicting HCC development where the receiver operating characteristic curve arrived above 0.95 for distinguishing healthy controls and patients with liver diseases (HD or HCC) and the ROC curve arrived at 0.85 for distinguishing HD and HCC. Our work not only developed a new method for the large-scale characterization of serum N-glycans but also provided valuable guidance for accurate and highly sensitive diagnosis of early liver cancer development in a non-invasive manner.

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“…Metal–organic frameworks (MOFs) are a type of porous material constructed by the self-assembly of metal ions/clusters and organic ligands. Since the pioneering report of Yaghi and co-workers in 1999, MOFs have stimulated a significant research output in various fields, ranging from gas storage/separation, heterogeneous catalysis, and drug delivery to chemical sensing and beyond ( Li et al, 1999 ; Li et al, 2018 ; Liu Q et al, 2019 ; Hu et al, 2022 ; Wang J et al, 2022 ). To date, more than 20,000 MOFs have been reported ( Gharagheizi et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…Then, the periodic constitution and flexible synthesis of MOFs also make it convenient for subsequent modification on other substrates, to confer additional characteristics and broaden their practical application ( Liu B et al, 2019 ; Hu et al, 2022 ). Besides, most MOFs feature a uniform structure, ultrahigh specific surface area, and sustainable pores, allowing them to provide more accessible binding sites and transfer channels for a specific target, which increases the host–guest interaction for enrichment ( Hu et al, 2022 ; Wang J et al, 2022 ). Owing to these advantages, hydrophilic MOFs have emerged as one of the most popular alternatives in HILIC.…”

Section: Introductionmentioning

confidence: 99%

Advances in hydrophilic metal–organic frameworks for N-linked glycopeptide enrichment

Wei

Wang

et al. 2022

Front. Chem.

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The comprehensive profiling of glycoproteins is of great significance for the timely clinical diagnosis and therapy. However, inherent obstacles hamper their direct analysis from biological samples, and specific enrichment prior to analysis is indispensable. Among the various approaches for glycopeptide enrichment, hydrophilic interaction liquid chromatography (HILIC) has attracted special focus, especially for the development of novel hydrophilic materials, which is the key of HILIC. Metal–organic frameworks (MOFs) are a type of porous materials constructed from the self-assembly of metal and organic linkers. Advantages such as high surface area, flexible pore size, and easy modification render hydrophilic MOFs as ideal candidates for HILIC, which has inspired many studies over the past years. In this review, advances in hydrophilic MOFs for N-linked glycopeptide enrichment are summarized. According to the synthesis strategies, those materials are categorized into three classes, namely pristine MOFs, MOFs with chemical modifications, and MOFs-derived composite. In each categorization, the preparation and the function of different moieties are covered, as well as the enrichment performances of sensitivity, selectivity, and practical application. Finally, a summary and future perspective on the applications of hydrophilic MOFs for N-linked glycopeptide enrichment are briefly discussed. This review is expected to raise awareness of the properties of hydrophilic MOFs and offer some valuable information to further research in glycoproteomics.

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A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides

Abstract: A novel MOFs-303-functionalized magnetic probe with strong hydrophilicity was synthesized for the highly efficient analysis of N-linked glycopeptides and the discovery of potential biomarkers with hepatocellular carcinoma.

Cited by 18 publications

References 43 publications

In situ grown magnetic COF@MOF with a phosphoserine anchor for in-depth N-glycopeptide analysis in serum

In situ grown magnetic COF@MOF with a phosphoserine anchor for in-depth N-glycopeptide analysis in serum

Novel Three-Dimensional Hierarchical Porous Carbon Probe for the Discovery of N-Glycan Biomarkers and Early Hepatocellular Carcinoma Detection

Advances in hydrophilic metal–organic frameworks for N-linked glycopeptide enrichment

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