Fabrication of material-independent morphology gradients for high-throughput applications

Künzler, Tobias P.; Drobek, Tanja; Sprecher, Christoph M.; Schüler, Martin; Spencer, Nicholas D.

doi:10.1016/j.apsusc.2006.04.014

Cited by 47 publications

(30 citation statements)

References 20 publications

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“…Notable examples include the use of gradients for studying wettability effects, protein adsorption and cell response. One previous study demonstrated that rat calvarial osteoblasts proliferated much faster on the surface with higher roughness and the projection area of osteoblasts increase considerably as the roughness decreased on the aluminum surface with gradation in roughness 2, 3. A separate study demonstrated that on a surface with silica particle‐density gradients, the same type of cells displayed a dramatic decrease in proliferation at locations with higher particle coverage and spreading and formation of a strong actin network was hindered at locations with maximum particle density while cells spread well and developed a well‐organized actin network in the absence of particles 4, 5…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Nanofiber Scaffolds With Gradations in Fiber Organization and Their Potential Applications

Xie

Michael

et al. 2012

Macromolecular Bioscience

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A new and simple method for fabrication of nanofiber scaffolds with gradations in fiber organization is reported. The nanofiber organization, achieved by deposition of random fibers on the uniaxially-aligned nanofiber mat in a gradient manner, directed morphological changes of applied adipose-derived stem cells. These morphological changes and resultant biochemical changes can help mimic the structural orientation of complex biomechanical structures like the collagen fiber structure at the tendon-to-bone insertion site. In addition, chemical gradients can be established through nanoencapsulation in this novel scaffold allowing for enhanced biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Nanofiber Scaffolds With Gradations in Fiber Organization and Their Potential Applications

Xie

Michael

et al. 2012

Macromolecular Bioscience

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“…Lithography is a very powerful approach for the production of surfaces with selected morphologies and nanostructures while keeping constant their chemical properties . As a standard process in the semiconductor industry for producing integrated circuits, it has a high level of spatial resolution and accuracy and can generate very high‐density topographical cues on single chips for cell surface interactions . The versatility of lithographic techniques lies in the possibility of integrating them with lift‐off to obtain patterned thin films or exploit them to create libraries of PVD deposited materials in a microarray format .…”

Section: Current Methods To Study Cell–surface Interactions: Nanostrumentioning

confidence: 99%

Micro‐nanopatterning as tool to study the role of physicochemical properties on cell–surface interactions

Singh

Patil

Thombre

et al. 2013

J Biomedical Materials Res

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The current nano-biotechnologies interfacing synthetic materials and cell biology requires a better understanding of cell-surface interactions on the micro-to-nanometer scale. Cell-substrate interactions are mediated by the presence of proteins adsorbed from biological fluids to the substrate. The effect of nanotopography and surface chemistry on protein adsorption as well as the mediation effect on subsequent cellular communication with substratum is not well documented. This review discusses the role of physicochemical properties of cell-surface interactions and state-of-the-art methods currently available for micro-nanoscale surface fabrication and patterning. We also briefly discuss the current surface patterning techniques that allow the combination of a fine and independent control on nanotopography and chemistry to understand the effect of surface nanoscale substrate morphology on cell-surface interactions which has never been realized in previous reports. In addition, we discuss the influence of various chemical patterning and modulation of the nano-topography of surfaces on cell functionality and phenotype.

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“…For analysis, a plane fit was applied to the whole data set and then individual profiles were fitted with a polynomial of degree four. 26 The roughness parameters were calculated for each profile according to DIN EN ISO 4288-98.…”

Section: Confocal White Light Microscopymentioning

confidence: 99%

“…13,[19][20][21][22][23][24][25][26] in this study, we describe for the very first time the production and use of samples with two different topographies (dual-type) on the same specimen ( Fig. 2).…”

Section: Introductionmentioning

confidence: 99%

Fabrication of TiO₂‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays

Schüler

Künzler

Wild

et al. 2008

J Biomedical Materials Res

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The goal of this study was to reproducibly generate samples with complex surface topographies and chemistries identical to a "master surface" and to test their response in cell culture using rat calvarial cells. Negative replicas of dual-type topography were fabricated using dental impression material with half of the surface exhibiting smooth and rough topography, respectively. Positive epoxy resin replicas were cast from the same negative replica eight times consecutively and coated with a 60-nm thin film of titanium dioxide using a vapor deposition technique. Atomic force microscopy, scanning electron microscopy, confocal white light microscopy, and X-ray photoelectron spectroscopy indicated that TiO(2)-coated epoxy replicas had surface topographical features and surface compositions nearly indistinguishable from the original titanium master surfaces. The described technique showed high reproducibility over at least eight generations of replication using the same negative replica. Rat calvarial osteoblasts proliferated just as well on dual topography surfaces as on single topography surfaces. The advantage of the dual-type substrates is that they facilitate comparison within a single culture dish, thus eliminating dish-to-dish variation as well as saving material, time and costs compared to the usual method of evaluating surfaces in separate dishes.

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Fabrication of material-independent morphology gradients for high-throughput applications

Cited by 47 publications

References 20 publications

Fabrication of Nanofiber Scaffolds With Gradations in Fiber Organization and Their Potential Applications

Fabrication of Nanofiber Scaffolds With Gradations in Fiber Organization and Their Potential Applications

Micro‐nanopatterning as tool to study the role of physicochemical properties on cell–surface interactions

Fabrication of TiO₂‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays

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Fabrication of material-independent morphology gradients for high-throughput applications

Cited by 47 publications

References 20 publications

Fabrication of Nanofiber Scaffolds With Gradations in Fiber Organization and Their Potential Applications

Fabrication of Nanofiber Scaffolds With Gradations in Fiber Organization and Their Potential Applications

Micro‐nanopatterning as tool to study the role of physicochemical properties on cell–surface interactions

Fabrication of TiO2‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays

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Product

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About

Fabrication of TiO₂‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays