Fabrication of mineralized electrospun PLGA and PLGA/gelatin nanofibers and their potential in bone tissue engineering

Meng, Zhaoxu; Li, H.F.; Sun, Zhaohao; Zheng, Wei; Zheng, Yufeng

doi:10.1016/j.msec.2012.10.021

Cited by 84 publications

(49 citation statements)

References 29 publications

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“…102 Several methods have been used for porous scaffold fabrication, including molding, 57 foaming, 21 leaching, 64 template-casting, 45 machining, 27 layer-by-layer assembly (LBL), 71 lithographic techniques, 67 and additive manufacturing. 107 Metals, such as titanium, 18 tantalum, 118 and magnesium, 109 ceramics such as calcium phosphate, 113 and polymers ranging from polyesters, 79 polyurethanes, 15 and polycarbonates, 65 to more specialized chemistries, such as polyanhydrides, 99 polyphosphazenes, 86 and polypropylene fumarates, 23 have provided a basic array of materials that can be used for scaffold fabrication. By themselves, however, rigid scaffold frameworks cannot promote the full biofunctionality that a vascularized BTE construct requires.…”

Section: Tissue Engineering Approaches For Vascularized Bone Repairmentioning

confidence: 99%

Vascularization in Bone Tissue Engineering Constructs

et al. 2015

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Vascularization of large bone grafts is one of the main challenges of bone tissue engineering (BTE), and has held back the clinical translation of engineered bone constructs for two decades so far. The ultimate goal of vascularized BTE constructs is to provide a bone environment rich in functional vascular networks to achieve efficient osseointegration and accelerate restoration of function after implantation. To attain both structural and vascular integration of the grafts, a large number of biomaterials, cells, and biological cues have been evaluated. This review will present biological considerations for bone function restoration, contemporary approaches for clinical salvage of large bone defects and their limitations, state-of-the-art research on the development of vascularized bone constructs, and perspectives on evaluating and implementing novel BTE grafts in clinical practice. Success will depend on achieving full graft integration at multiple hierarchical levels, both between the individual graft components as well as between the implanted constructs and their surrounding host tissues. The paradigm of vascularized tissue constructs could not only revolutionize the progress of bone tissue engineering, but could also be readily applied to other fields in regenerative medicine for the development of new innovative vascularized tissue designs.

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Section: Tissue Engineering Approaches For Vascularized Bone Repairmentioning

confidence: 99%

Vascularization in Bone Tissue Engineering Constructs

et al. 2015

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“…In the first method, scaffolds often have low mechanical properties, but in the second one, higher mechanical properties would be gained. Co‐spinning of the suspensions of HA and polymer becomes impossible by increasing the hydroxyapatite content, because the electrospinning process is always blocked . Poly( l ‐lactic) acid is one of the synthetic polymers with high mechanical properties and good processibility, biodegradability and biocompatibility which is mostly used in tissue engineering .…”

Section: Introductionmentioning

confidence: 99%

Surface mineralized hybrid nanofibrous scaffolds based on poly(l‐lactide) and alginate enhances osteogenic differentiation of stem cells

Ataie

Shabani

Seyedjafari

2018

J Biomedical Materials Res

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Surface mineralized nanofibrous scaffolds hold great potential for bone tissue engineering applications. In this study, a new hybrid nanofibrous scaffold composed of alginate/poly(l‐lactide) nanofibers was fabricated using electrospinning method and then crosslinking process was employed. Hydroxyapatite crystal formation took place using in situ precipitation by immersion of the scaffolds in simulated body fluid solution for 10 days at 37°C. The morphologies of the scaffolds were observed using scanning electron microscope. Hydroxyapatite crystal formation on the surface of nanofibers was confirmed using scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, Fourier transform infrared spectroscopy and X‐ray diffraction analysis. The biocompatibility of the fabricated scaffolds was evaluated using mesenchymal stem cell culture and MTT assay. According to alkaline phosphatase activity and calcium content assays, it was concluded that the osteogenic differentiation of stem cells was considerable on scaffolds containing hydroxyapatite crystals in comparison with other specimens. The results showed biocompatibility of the scaffolds and their support from stem cell adhesion, growth and osteogenic differentiation, so the hydroxyapatite‐coated poly(l‐lactide)/alginate hybrid nanofibrous scaffolds hold suitable characteristics for bone tissue engineering applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 586–596, 2019.

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“…These composite materials can adapt well to the internal environment of humans [5]. Gel is rich in amino and carboxyl hydrophilic groups [6] and is beneficial for nutrients and oxygen infiltration. Chitosan (CS) possesses good biocompatibility, antimicrobial properties [7], and has the potential for various chemical modifications and combinations to obtain specific properties [8].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Gelatin-Based Electrospun Composite Fibers for Anti-Bacterial Properties and Protein Adsorption

Gao

Wang

et al. 2016

Marine Drugs

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A major goal of biomimetics is the development of chemical compositions and structures that simulate the extracellular matrix. In this study, gelatin-based electrospun composite fibrous membranes were prepared by electrospinning to generate bone scaffold materials. The gelatin-based multicomponent composite fibers were fabricated using co-electrospinning, and the composite fibers of chitosan (CS), gelatin (Gel), hydroxyapatite (HA), and graphene oxide (GO) were successfully fabricated for multi-function characteristics of biomimetic scaffolds. The effect of component concentration on composite fiber morphology, antibacterial properties, and protein adsorption were investigated. Composite fibers exhibited effective antibacterial activity against Staphylococcus aureus and Escherichia coli. The study observed that the composite fibers have higher adsorption capacities of bovine serum albumin (BSA) at pH 5.32–6.00 than at pH 3.90–4.50 or 7.35. The protein adsorption on the surface of the composite fiber increased as the initial BSA concentration increased. The surface of the composite reached adsorption equilibrium at 20 min. These results have specific applications for the development of bone scaffold materials, and broad implications in the field of tissue engineering.

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Fabrication of mineralized electrospun PLGA and PLGA/gelatin nanofibers and their potential in bone tissue engineering

Cited by 84 publications

References 29 publications

Vascularization in Bone Tissue Engineering Constructs

Vascularization in Bone Tissue Engineering Constructs

Surface mineralized hybrid nanofibrous scaffolds based on poly(l‐lactide) and alginate enhances osteogenic differentiation of stem cells

Fabrication of Gelatin-Based Electrospun Composite Fibers for Anti-Bacterial Properties and Protein Adsorption

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