Fabrication of Nanohydroxyapatite/Poly(caprolactone) Composite Microfibers Using Electrospinning Technique for Tissue Engineering Applications

Abstract

Tissue engineering fibrous scaffolds serve as three-dimensional (3D) environmental framework by mimicking the extracellular matrix (ECM) for cells to grow. Biodegradable polycaprolactone (PCL) microfibers were fabricated to mimic the ECM as a scaffold with 7.5% (w/v) and 12.5% (w/v) concentrations. Lower PCL concentration of 7.5% (w/v) resulted in microfibers with bead defects. The average diameter of fibers increased at higher voltage and the distance of tip to collector. Further investigation was performed by the incorporation of nanosized hydroxyapatite (nHA) into microfibers. The incorporation of 10% (w/w) nHA with 7.5% (w/v) PCL solution produced submicron sized beadless fibers. The microfibrous scaffolds were evaluated using various techniques. Biodegradable PCL and nHA/PCL could be promising for tissue engineering scaffold application.

The effect of collector type on the physical, chemical, and biological properties of polycaprolactone/gelatin/nano‐hydroxyapatite electrospun scaffold

“…Various biodegradable polymers such as polylactic acid (PLA), poly‐glycolic acid (PGA), polylactic‐co‐glycolic acid (PLGA) and polycaprolactone (PCL) are selected for tissue engineering, among which, PCL is more appropriate due to its cost‐effective price, ability to be dissolved in most organic solvents and compatibility with typical used drugs in orthopedic, drug release and bone tissue engineering applications . The lack of bioactivity on these bioabsorbable polymers is a problem due to lack cellular recognition sites . Casa et al , in their study investigated PCL/HA scaffolds composites.…”

Incorporation of nanohydroxyapatite and vitamin D3 into electrospun PCL/Gelatin scaffolds: The influence on the physical and chemical properties and cell behavior for bone tissue engineering

“…PCL electrospinning, alone or in combination with other natural or synthetic polymers, is used for the production of nanofiber scaffolds for bone, cartilage, nerve, blood vessels, skin, etc. Although PCL has good mechanical strength, lack of surface cell recognition sites and weak hydrophilicity have restricted its use for the cellular adhesion, proliferation, and differentiation . Therefore, this polymer is used in combination with natural polymers such as gelatin.…”

“…Although PCL has good mechanical strength, lack of surface cell recognition sites and weak hydrophilicity have restricted its use for the cellular adhesion, proliferation, and differentiation. 4 Therefore, this polymer is used in combination with natural polymers such as gelatin.…”

Electrospun Polycaprolactone (PCL) and PCL/ nano-hydroxyapatite (PCL/nHA)-based nanofibers for bone tissue engineering application

“…Electrospinning is able to yield large amount of nanofibers with high specific surface areas and high porosity from a huge variety of bio-polymers [5]. These nanofibers are able to mimic the natural extracellular matrix (ECM) and thus enhance cellular behavior [6].…”