Fabrication of Plga/Hap and Plga/Phb/Hap Fibrous Nanocomposite Materials for Osseous Tissue Regeneration

Abstract

The study presents the manufacturing of nanofibrous structures as osteoconductive, osteoinductive materials for osseous tissue regeneration. The fibrous structures were obtained by electrospinning of poly(l-lactide-coglicolide) (PLGA) with addition of hydroxyapatite (HAp) and of a blend of PLGA with polyhydroxybutyrate with HAp added. The polymers used in the experiment were synthesised by an innovative method with a zirconium catalyst. First, the optimal electrospinning process parameters were selected. For the characterisation of the obtained osseous tissue reconstruction materials, the physical, macroscopic, functional, mechanical and thermal properties as well as crystallinity index were studied. The study of the radiation sterilisation influence on average molar mass, thermal and mechanical properties was made in order to analyse the degradation effect.

Biological Properties of Low-Toxicity PLGA and PLGA/PHB Fibrous Nanocomposite Implants for Osseous Tissue Regeneration. Part I: Evaluation of Potential Biotoxicity

“…In the case of forming fibres from PLGA solutions, the obtained fibres had an average size of 0.47 µm, whereas in the case of forming fibres from PLGA/PHB solutions, the obtained fibres had an average size of 0.18 µm. The selection of the optimal forming conditions of the discussed nanofibers was conducted according to the data presented in paper [ 39 ], properties of non-woven fabric layers constituting the implant, are shown in Table 3 .…”

Application of the melt-blown technique in the production of shape-memory nonwoven fabrics from a blend of poly(L-lactide) and atactic poly[(R,S)-3-hydroxy butyrate]

“…The tested biocompatibility revealed excellent cell proliferation of the materials with addition of amorphous polyhydroxybutyrate (PHB). 28 According to other publications concerning PLLA-a-PHB blend, [29][30][31] it is considered to be a thermoplastic material with fiber-forming properties. The addition of a-PHB to the PLA changes its structural and thermal properties.…”

Production of PHB Scaffolds Reinforced with HAp Through Electrospinning

“…This member of the apatite family exhibits properties that render it suitable for various applications, including serving as a synthetic bone substitute, creating extracellular supports/ matrixes for tissue engineering involving cytokines, bone, and cartilage cells, and acting as a promoter for improving cell adhesion and dissemination on the surfaces of membranes designed for tissue engineering applications [30][31][32][33] Numerous studies have concentrated on producing PHB/ HAp scaffolds for applications in bone tissue engineering, with the aim of achieving bone regeneration at sites affected by damage or injury 29,34 . Other research has investigated the electrospinning of PHB and HAp in combination with polymers like polylactic acid (PLLA) 35 and poly(lactic acidco-glycolic acid) (PLGA) 36,37 .…”