Fabrication of poly(glycerol sebacate) fibrous membranes by coaxial electrospinning: Influence of shell and core solutions

You, Zhi Rong; Hu, Ming; Tuan-Mu, Ho Yi; Hu, Jin

doi:10.1016/j.jmbbm.2016.06.022

Cited by 31 publications

(25 citation statements)

References 30 publications

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“…The in vitro cytocompatibility of the PGS membrane before and after the removal of PVA was assessed quantitatively by the Alamar Blue and the lactate dehydrogenase (LDH) assays using HUASMCs cultured in direct contact with the membrane. 15 Briefly, for the Alamar Blue assay, HUASMCs were seeded in a 48-well plate at a density of 2.5 3 10 4 per well. Upon cell adhesion, a piece of membrane (2.5 mg) was added in the well.…”

Section: Cytocompatibilitymentioning

confidence: 99%

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Fabrication of a mechanically anisotropic poly(glycerol sebacate) membrane for tissue engineering

et al. 2017

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Poly(glycerol sebacate) (PGS) has been used successfully as a scaffolding material for soft tissue engineering. PGS scaffolds, however, are usually mechanically isotropic, which may restrict their use in tissue repairs as many soft tissues in the body have anisotropic mechanical behaviors. Although various methods have been used to fabricate anisotropic scaffolds, it remains challenging to make anisotropic scaffolds from thermoset PGS. Here a new, simple method to fabricate an anisotropic PGS membrane which can then be used to construct thicker three-dimensional anisotropic scaffolds was developed. First, an aligned sacrificial poly(vinyl alcohol) fibrous membrane was prepared by electrospinning. The fibrous membrane was then partially immersed in PGS prepolymer solution, resulting in a composite membrane upon drying. After curing, the sacrificial fibers within the membrane were removed by water, supposedly leaving aligned cylindrical pores in the membrane. Both SEM and AFM illustrated aligned grooves on the surface of the resultant PGS membrane, indicating the successful removal of sacrificial fibers. The PGS membrane was validated to be mechanically anisotropic using uniaxial tensile testing along and perpendicular to the predominant pore direction. The in vitro cytocompatibility of the PGS membrane was confirmed. As a demonstration of its potential application in vascular tissue engineering, a tubular scaffold was constructed by wrapping a stack of two axisymmetric pieces of the anisotropic PGS membranes on a mandrel. The compliance of the scaffold was found to depend on the pitch angle of its double helical structure, imitating the anisotropic mechanical behavior of the arterial media. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 760-770, 2018.

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Section: Cytocompatibilitymentioning

confidence: 99%

“…Because pPGS melts during curing, it remains challenging to make anisotropic PGS scaffolds using the aforementioned methods. Recently, PGS fibrous membranes were fabricated by coaxial electrospinning [11][12][13][14][15] and blend electrospinning. 16 PGS fibrous membranes with varying degrees of fiber alignment were also realized.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a mechanically anisotropic poly(glycerol sebacate) membrane for tissue engineering

et al. 2017

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“…Yi and LaVan reported a coaxial electrospinning process, to prepare PGSeb fibers using PLLA as a protective shell which was removed after the PGSeb prepolymer core was cured (Figure 6g) [132]. Alternatively, PLA-poly(ethylene oxide) (PEO) [139] and PVA have been used [140,141]. PGSeb fibrous sheets were also obtained by electrospinning a PVA/PGSeb prepolymer solution in 1,1,1,3,3,3-hexafluoroisopropanol [142].…”

Section: Processingmentioning

confidence: 99%

Polyglycerol Hyperbranched Polyesters: Synthesis, Properties and Pharmaceutical and Biomedical Applications

Zamboulis

Nakiou

Christodoulou

et al. 2019

IJMS

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In a century when environmental pollution is a major issue, polymers issued from bio-based monomers have gained important interest, as they are expected to be environment-friendly, and biocompatible, with non-toxic degradation products. In parallel, hyperbranched polymers have emerged as an easily accessible alternative to dendrimers with numerous potential applications. Glycerol (Gly) is a natural, low-cost, trifunctional monomer, with a production expected to grow significantly, and thus an excellent candidate for the synthesis of hyperbranched polyesters for pharmaceutical and biomedical applications. In the present article, we review the synthesis, properties, and applications of glycerol polyesters of aliphatic dicarboxylic acids (from succinic to sebacic acids) as well as the copolymers of glycerol or hyperbranched polyglycerol with poly(lactic acid) and poly(ε-caprolactone). Emphasis was given to summarize the synthetic procedures (monomer molar ratio, used catalysts, temperatures, etc.,) and their effect on the molecular weight, solubility, and thermal and mechanical properties of the prepared hyperbranched polymers. Their applications in pharmaceutical technology as drug carries and in biomedical applications focusing on regenerative medicine are highlighted.

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“…It has been identified to be well biocompatible, flexible and degradable, which are widely used as kinds of patch of organs and wounds [25]. However, PGS has low specific surface area and low spinnablility, which cannot support the formation of fibrous morphology, and further restrict cells attachment and migration for tissues or wounds regeneration [26]. Thus, using tissue scaffolds with micro-nanofibers can provide more space to go deep into scaffolds for cells attachment and migration [27].…”

Section: Introductionmentioning

confidence: 99%

“…Coaxial electrospinning is a novel method of the fabrication of micro-/nanofibers, and it is facile and high effective to produce fibrous membranes with a core-shell structure, which can integrate more than two different materials with synergistic effect [26,34]. The prepared core-shell fibers were normally applied to tissue scaffolds [35,36], drugs controlled release [9,18,37], and antibacterial application [38].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Core–Shell Fibrous 2D Scaffold with Biocompatible Poly(Glycerol Sebacate) and Poly-l-Lactic Acid for Wound Healing

Yang

et al. 2020

Adv. Fiber Mater.

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Biomimetic scaffolds made by synthetic materials are usually used to replace the natural tissues aimed at speeding up the skin regeneration. In this study, a flexible and cytocompatible poly(glycerol sebacate)@poly-l-lactic acid (PGS@PLLA) fibrous scaffold with a core-shell structure was fabricated by coaxial electrospinning, where the shell PLLA was used to be a skeleton with pores on the fibrous surface. The fibrous morphology with pores on the surface of the prepared fibers was observed by SEM. The core-shell microstructure of PGS@PLLA fibers was confirmed by TEM and Laser Scanning Confocal Microscopy (LSCM). In addition, the prepared fibers exhibited a strong ability to repair tissues of the skin wound, where the stability of cell security and proliferation, and the lower inflammatory response were all superior to those of pure PLLA scaffold. It's worth noting that the percentage of skin tissue was regenerated by 95% within 14 days, which suggests the potential application for electrospun-based synthetic fibrous scaffolds on wound healing.

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Fabrication of poly(glycerol sebacate) fibrous membranes by coaxial electrospinning: Influence of shell and core solutions

Cited by 31 publications

References 30 publications

Fabrication of a mechanically anisotropic poly(glycerol sebacate) membrane for tissue engineering

Fabrication of a mechanically anisotropic poly(glycerol sebacate) membrane for tissue engineering

Polyglycerol Hyperbranched Polyesters: Synthesis, Properties and Pharmaceutical and Biomedical Applications

Electrospun Core–Shell Fibrous 2D Scaffold with Biocompatible Poly(Glycerol Sebacate) and Poly-l-Lactic Acid for Wound Healing

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