Fabrication of poly(vinyl alcohol) hydrogel beads crosslinked using sodium sulfate for microorganism immobilization

Takei, Takayuki; Ikeda, Kaoru; Ijima, Hiroyuki; Kawakami, Koei

doi:10.1016/j.procbio.2010.10.011

Cited by 105 publications

(74 citation statements)

References 20 publications

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“…However, rising sodium sulfate concentrations deteriorated the ethanol yields. This could be associated with the substantial mortality of the entrapped cells (Takei et al, 2011). The results of this study were consistent with previous reports.…”

Section: Discussionsupporting

confidence: 93%

“…Moreover, immobilization of S. cerevisiae NBRC-0216 in PVAalginate hydrogel was examined. The results indicate that cell viability appears to be diminished with the increase of sodium sulfate concentration owing to osmotic stress and denaturation of several proteins in the yeast cells (Takei et al, 2011).…”

Section: Discussionmentioning

confidence: 90%

“…The beads were incubated for 30 min and then immersed in 0.5 M Na 2 SO 4 for 1.5 h. The beads were hardened by incubation in 0.1 M CaCl 2 for 24 h at 4 °C. Prior to use as biocatalysts for ethanol fermentation, the beads were washed with sterile distilled water to remove excess amounts of calcium ions and unentrapped cells (Quintana and Dalton, 1998;Takei et al, 2011).…”

Section: Entrapment Of K Marxianus Dbkkuy-103 In Pva Hydrogelmentioning

confidence: 99%

“…However, it reduces cell viability, and PVA beads usually agglomerate due to the highly acidic conditions and slow cross-linking process (Hashimoto and Furukawa, 1987;Wu and Wisecarver, 1992). To overcome these problems, the addition of sodium alginate to the PVA beads and the treatment of the beads with sodium sulfate were examined (Takei et al, 2011;Zain et al, 2011). Therefore, optimal conditions for the preparation of the beads are necessary to achieve high stability of hydrogel beads.…”

Section: Introductionmentioning

confidence: 99%

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Efficient entrapment of Kluyveromyces marxianus DBKKUY-103 in polyvinyl alcohol hydrogel for ethanol production from sweet sorghum juice

Nonthasen¹,

Piyatheerawong²,

Thanonkeo³

2015

Turk J Biol

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The aim of this investigation was to optimize immobilization conditions to entrap Kluyveromyces marxianus DBKKUY-103 during ethanol fermentation from sweet sorghum juice using a response surface methodology. The effects and interactions of variables involved in cell entrapment using polyvinyl alcohol and sodium alginate were studied through a fractional factorial design. The results suggested that the primary variables involved in cell entrapment that significantly affected ethanol fermentation were sodium sulfate concentration, polyvinyl alcohol, and sodium alginate contents (P < 0.01). Subsequently, these variables were optimized for the preparation of cell entrapment based on response surface methodology and using central composite design. The results indicate that cell entrapment can achieve an ethanol concentration of 81.56 g L -1 under the following optimal conditions: bead gel diameter of 4.0 mm, calcium chloride concentration of 0.3 M, polyvinyl alcohol content of 10.09% (w v -1 ), sodium alginate content of 3.39% (w v -1 ), and sodium sulfate concentration of 0.44 M. Furthermore, statistical analysis of the model reveals that it adequately fit the experimental data (P < 0.01, R 2 = 0.9879). Therefore, this model can be used to efficiently prepare entrapped cells for ethanol production.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 90%

Section: Entrapment Of K Marxianus Dbkkuy-103 In Pva Hydrogelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficient entrapment of Kluyveromyces marxianus DBKKUY-103 in polyvinyl alcohol hydrogel for ethanol production from sweet sorghum juice

Nonthasen¹,

Piyatheerawong²,

Thanonkeo³

2015

Turk J Biol

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“…The degradation of PVA contained in dyeing wastewater by pure or mixed microorganisms had also been reported (Kim et al 1997;Choi et al 2004). To improve the efficiency of dyestuff biodegradation, such microbial immobilization technologies using polyvinylidene fluoride (Al-Zuhair and El-Nass 2011), PVA (Takei et al 2011), alginate (Chen et al 2010), and polyethylene glycol (PEG) (Kim et al 2013) have been receiving considerable attention.…”

Section: Introductionmentioning

confidence: 99%

Enhanced bioremoval of refractory compounds from dyeing wastewater using optimized sequential anaerobic/aerobic process

Bae

Han

Kim

et al. 2016

Int. J. Environ. Sci. Technol.

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The upflow anaerobic sludge blanket process followed by the biological aerated filter process was employed to improve the removal of color and recalcitrant compounds from real dyeing wastewater. The highest removal efficiency for color was observed in the anaerobic process, at 8-h hydraulic retention time, seeded with the sludge granule. In the subsequent aerobic process packed with the microbe-immobilized polyethylene glycol media, the removal efficiency for chemical oxygen demand increased significantly to 75 %, regardless of the empty bed contact time. The average influent non-biodegradable soluble chemical oxygen demand was 517 mg/L, and the average concentration in effluent from the anaerobic reactor was 363 mg/L, suggesting the removal of some recalcitrant matters together with the degradable ones. The average nonbiodegradable soluble chemical oxygen demand in effluent from the aerobic reactor was 87, 93, and 118 mg/L, with the removal efficiency of 76, 74, and 67 %, at 24-, 12-, and 8-h empty bed contact time, respectively. The combined anaerobic sludge blanket and aerobic cell-entrapped process was effective to remove the refractory compounds from real dyeing wastewater as well as in reducing organic loading to meet the effluent discharge limits. This integrated process is considered an effective and economical treatment technology for dyeing wastewater.

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Study on preparation and properties of PVA‐SA‐PHB‐AC composite carrier for microorganism immobilization

Ren

Wei

2013

J of Applied Polymer Sci

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Polyvinyl alcohol(PVA) bead crosslinked with boric acid has been widely utilized as a microorganism immobilization carrier. However, it has some disadvantages such as drastic cell viability loss, small adsorption capacity and mass transfer limitation. To improve upon these drawbacks, a new method to prepare PVA composite pieces with the addition of activated carbon (AC) and poly‐3‐hydroxybutyrate(PHB) was explored through a combination of freezing/thawing and the boric acid method and by using Tween‐80 to improve the mass transfer performance of hydrophobic organics. m‐Cresol and pyrene were used as representative compounds with benzene ring structures to model hydrophilic and hydrophobic organics in order to test the performance of PVA pieces. The results showed that, compared with the boric acid method alone, a combination of freezing/thawing and the boric acid method led to a decrease in total organic carbon(TOC) loss from 0.315 g g−1 to 0.033 g g−1 and increased the oxygen uptake rate(OUR) of microorganisms from 0.03 mg L−1·min−1 to 0.22 mg L−1 min−1. The m‐cresol equilibrium adsorption amount of the PVA‐SA(sodium alginate)‐PHB‐AC piece was 2.80 times that of the PVA‐SA piece. The diffusion coefficient of pyrene in the PVA‐SA‐PHB‐AC piece increased from 0.53×10−9 m2 min−1 to 2.30×10−9 m2 min−1 with increasing concentrations of Tween‐80 from 1000 mg L−1 to 5000 mg L−1. The PVA‐SA‐PHB‐AC composite carrier demonstrated great scope for immobilizing microorganisms for practical wastewater bio‐treatment. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 39837.

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Fabrication of poly(vinyl alcohol) hydrogel beads crosslinked using sodium sulfate for microorganism immobilization

Cited by 105 publications

References 20 publications

Efficient entrapment of Kluyveromyces marxianus DBKKUY-103 in polyvinyl alcohol hydrogel for ethanol production from sweet sorghum juice

Efficient entrapment of Kluyveromyces marxianus DBKKUY-103 in polyvinyl alcohol hydrogel for ethanol production from sweet sorghum juice

Enhanced bioremoval of refractory compounds from dyeing wastewater using optimized sequential anaerobic/aerobic process

Study on preparation and properties of PVA‐SA‐PHB‐AC composite carrier for microorganism immobilization

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