Fabrication of self-assembling peptide nanofiber hydrogels for myocardial repair

Yuan, Xingliang; He, Bin; Lv, Zi; Luo, Suxin

doi:10.1039/c4ra08582e

Cited by 22 publications

(12 citation statements)

References 142 publications

(337 reference statements)

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“…This study confirmed that the introduction of conductive materials in nanofibrous scaffold increase their potential to mimic natural ECM especially in terms of their electrical conduction [42]. Further step ahead, small molecules like peptides [133] and functionalized gold nanoparticles were integrated in nanofibrous scaffold of polymethylglutarimide (PMGI), these gold nanoparticles were functionalized with adhesive peptides to enhance the cell attachment. Additionally, these scaffolds were also found to promote the differentiation of hiPSCs towards cardiomyocytes [134].…”

Section: Nanofiberssupporting

confidence: 63%

Current research trends and challenges in tissue engineering for mending broken hearts

et al. 2019

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Cardiovascular disease (CVD) is among the leading causes of mortality worldwide. The shortage of donor hearts to treat end-stage heart failure patients is a critical problem. An average of 3,500 heart transplant surgeries are performed globally, half of these transplants are performed in the US alone. Stem cell therapy is growing rapidly as an alternative strategy to repair or replace the damaged heart tissue after a myocardial infarction (MI). Nevertheless, the relatively poor survival of the stem cells in the ischemic heart is a major challenge to the therapeutic efficacy of stem-cell transplantation. Recent advancements in tissue engineering offer novel biomaterials and innovative technologies to improve upon the survival of stem cells as well as to repair the damaged heart tissue following a myocardial infarction (MI). However, there are several limitations in tissue engineering technologies to develop a fully functional, beating cardiac tissue. Therefore, the main goal of this review article is to address the current advancements and barriers in cardiac tissue engineering to augment the survival and retention of stem cells in the ischemic heart.

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Section: Nanofiberssupporting

confidence: 63%

Current research trends and challenges in tissue engineering for mending broken hearts

et al. 2019

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“…Many previous studies have demonstrated that ionic self-complementary peptide-formed nanofiber scaffolds hold some promise in repairing damaged tissues or organs, including bone, 3 , 11 cartilage, 12 , 13 nerve, 14 , 15 heart, 16 , 17 as well as in wound healing. 18 , 19 The principle of molecular self-assembly guides the ionic self-complementary peptides to self-assemble into three-dimensional (3D) entangled nanofiber hydrogel scaffolds, which are analogous to those found in natural extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

Functionalized D-form self-assembling peptide hydrogels for bone regeneration

He¹,

Ou²,

Zhou³

et al. 2016

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Bone defects are very common in orthopedics, and there is great need to develop suitable bone grafts for transplantation in vivo. However, current bone grafts still encounter some limitations, including limited availability, immune rejection, poor osteoinduction and osteoconduction, poor biocompatibility and degradation properties, etc. Self-assembling peptide nanofiber scaffolds have emerged as an important substrate for cell culture and bone regeneration. We report on the structural features (eg, Congo red staining, circular dichroism spectroscopy, transmission electron microscopy, and rheometry assays) and osteogenic ability of d-RADA16-RGD peptide hydrogels (with or without basic fibroblast growth factor) due to the better stability of peptide bonds formed by these peptides compared with those formed by l-form peptides, and use them to fill the femoral condyle defect of Sprague Dawley rat model. The bone morphology change, two-dimensional reconstructions using microcomputed tomography, quantification of the microcomputed tomography analyses as well as histological analyses have demonstrated that RGD-modified d-form peptide scaffolds are able to enhance extensive bone regeneration.

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“…RADA is a class of SAPs that can form supramolecular hydrogels upon mixing with the positively charged multivalent ions. The RADA-based hydrogels are biocompatible and biodegradable, mimic the nanofibrous structure of ECM, induce cell recruitment, and allow for sequence modification to render other bioactive functions [50,51]. These hydrogels have been used for the engineering of various tissues with low immunogenicity [52][53][54].…”

Section: Discussionmentioning

confidence: 99%

A Cell-Free SDKP-Conjugated Self-Assembling Peptide Hydrogel Sufficient for Improvement of Myocardial Infarction

et al. 2020

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Biomaterials in conjunction with stem cell therapy have recently attracted attention as a new therapeutic approach for myocardial infarction (MI), with the aim to solve the delivery challenges that exist with transplanted cells. Self-assembling peptide (SAP) hydrogels comprise a promising class of synthetic biomaterials with cardiac-compatible properties such as mild gelation, injectability, rehealing ability, and potential for sequence modification. Herein, we developed an SAP hydrogel composed of a self-assembling gel-forming core sequence (RADA) modified with SDKP motif with pro-angiogenic and anti-fibrotic activity to be used as a cardioprotective scaffold. The RADA-SDKP hydrogel was intramyocardially injected into the infarct border zone of a rat model of MI induced by left anterior descending artery (LAD) ligation as a cell-free or a cell-delivering scaffold for bone marrow mesenchymal stem cells (BM-MSCs). The left ventricular ejection fraction (LVEF) was markedly improved after transplantation of either free hydrogel or cell-laden hydrogel. This cardiac functional repair coincided very well with substantially lower fibrotic tissue formation, expanded microvasculature, and lower inflammatory response in the infarct area. Interestingly, BM-MSCs alone or in combination with hydrogel could not surpass the cardiac repair effects of the SDKP-modified SAP hydrogel. Taken together, we suggest that the RADA-SDKP hydrogel can be a promising cell-free construct that has the capability for functional restoration in the instances of acute myocardial infarction (AMI) that might minimize the safety concerns of cardiac cell therapy and facilitate clinical extrapolation.

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Fabrication of self-assembling peptide nanofiber hydrogels for myocardial repair

Cited by 22 publications

References 142 publications

Current research trends and challenges in tissue engineering for mending broken hearts

Current research trends and challenges in tissue engineering for mending broken hearts

Functionalized D-form self-assembling peptide hydrogels for bone regeneration

A Cell-Free SDKP-Conjugated Self-Assembling Peptide Hydrogel Sufficient for Improvement of Myocardial Infarction

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