Fabrication of triblock ABA type peptide dendrimer based on glutamic acid dimethyl ester and PEG as a potential nano drug delivery agent

Abstract

Introduction: Peptide dendrimers build up from amino acids and they simulate to artificial proteins with globular architecture. These characteristics furnish peptide dendrimers with best biodegradability and biocompatibility in drug delivery systems. Methods: A barbell-like dendrimer from glutamic acid dimethyl ester-poly (ethylene glycol)-glutamic acid dimethyl ester as ABA-type triblock copolymer (PG-PEG-PG) was prepared with liquid-phase peptide synthesis via a divergent approach. PEG 600 diacid (PEG-A) and glutamic acid dimethyl ester were used as the core and the monomeric building blocks, respectively. Linear-dendritic copolymer was prepared in the presence of DCC in pyridine. Transmission electron microscope (TEM) was used for measuring the size of first generation (G1-COOH) and second generation (G2-COOH) of dendrimer compounds. Thermal behavior of the synthesized dendrimers was investigated using DSC. Results: The desired generations G1-COOH, G2-COOH and G3-COOH were prepared by divergent method using PEG diacid 600 as a core compound. The size range of the resulted particles was found to be 20-100 nm for various generations. The isolated dendrimer was examined as the drug-delivery agent and the controlled release was carried out for drug molecule in pH 7.4. Conclusion: Based on the obtained results, the synthesized biocompatible dendrimers could potentially be utilized as a drug carrier agent.

Dumbbell-like polyionic complexes of dendronized poly(ethylene glycol): synthesis and self-assembly studies

“…[28][29][30][31] PEG conjugates with dendrons based on natural amino and hydroxy acids deserve a special mention. [32][33][34][35][36] Due to their composition, such linear-dendritic copolymers demonstrate high biocompatibility and low toxicity, possess a large number of functional ionogenic groups on the periphery, and may be used for effective binding of various biologically active substances. 37 To date, tri-and diblock copolymers of PEG with lysine dendrons are the most studied ones.…”

Application or function of citric acid in drug delivery platforms

“…Designing efficient drug delivery systems (DDSs) represents one of the frontier concerns of science regarding human health care 15–19 . The upcoming bioactive molecules as therapeutic agents are related to the design of a suitable system for their delivery into the body.…”

“…[7][8][9][10][11][12][13][14] Designing efficient drug delivery systems (DDSs) represents one of the frontier concerns of science regarding human health care. [15][16][17][18][19] The upcoming bioactive molecules as therapeutic agents are related to the design of a suitable system for their delivery into the body. Proteins, peptides, oligonucleotides, and small bioactive molecules are unstable compounds that are required to be protected from degradation in the biological environment.…”

Co‐delivery of methotrexate and doxorubicin via nanocarriers of star‐like poly(DMAEMA‐block‐HEMA‐block‐AAc) terpolymers

“…27,28 Biodegradable hyperbranched polymers based on aliphatic polyester, poly(D,L-lactide) and polyaniline and five-arm and functionalized star-like polyaniline and polycaprolactone polymers with a D-glucose core have also been utilized as effective biomaterials. 29,30 Among these, dendritic nanocarriers are most favourable nanostructures because of the branching features [31][32][33][34] which are useful in multi-drug delivery systems. 35 Due to the easy deformation of linear polymers through the pores of the renal filtration membrane, their elimination via renal excretion is done easily.…”