Fabrication of versatile dynamic hyaluronic acid-based hydrogels

Shi, Wen; Hass, Blake; Kuss, Mitchell; Zhang, Haipeng; Ryu, Sangjin; Zhang, Dongze; Li, Tieshi; Li, Yu Long; Duan, Bin

doi:10.1016/j.carbpol.2019.115803

Cited by 110 publications

(102 citation statements)

References 78 publications

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“…To evaluate the effects of BMSCs on PC12 cell differentiation and proliferation, PC12 cells were cultured on the inner surface of four nerve conduits ( Table 1 ). Before bioprinting of nerve conduits, different cell trackers were used to fluorescently label BMSCs and PC12 cells for simultaneously visualization during cells co-culture [ 35 ] ( Fig. S2 ).…”

Section: Resultsmentioning

confidence: 99%

Additive-lathe 3D bioprinting of bilayered nerve conduits incorporated with supportive cells

Liu

Zhang

et al. 2021

Bioactive Materials

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Nerve conduits have been identified as one of the most promising treatments for peripheral nerve injuries, yet it remains unsolved how to develop ideal nerve conduits with both appropriate biological and mechanical properties. Existing nerve conduits must make trade-offs between mechanical strength and biocompatibility. Here, we propose a multi-nozzle additive-lathe 3D bioprinting technology to fabricate a bilayered nerve conduit. The materials for printing consisted of gelatin methacrylate (GelMA)-based inner layer, which was cellularized with bone marrow mesenchymal stem cells (BMSCs) and GelMA/poly(ethylene glycol) diacrylate (PEGDA)-based outer layer. The high viability and extensive morphological spreading of BMSCs encapsulated in the inner layer was achieved by adjusting the degree of methacryloyl substitution and the concentration of GelMA. Strong mechanical performance of the outer layer was obtained by the addition of PEGDA. The performance of the bilayered nerve conduits was assessed using in vitro culture of PC12 cells. The cell density of PC12 cells attached to cellularized bilayered nerve conduits was more than 4 times of that on acellular bilayered nerve conduits. The proliferation rate of PC12 cells attached to cellularized bilayered nerve conduits was over 9 times higher than that on acellular bilayered nerve conduits. These results demonstrate the additive-lathe 3D bioprinting of BMSCs embedded bilayered nerve conduits holds great potential in facilitating peripheral nerve repair.

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Section: Resultsmentioning

confidence: 99%

Additive-lathe 3D bioprinting of bilayered nerve conduits incorporated with supportive cells

Liu

Zhang

et al. 2021

Bioactive Materials

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“…The NPC cells showed good viability (85 ± 2% of living cells) after extrusion and maintained the excellent viability of 81 ± 2% of living cells after 3 days of culture. The results indicated that multifunctional injectable and ROS responsive self-healing HA-PBA-PVA dynamic hydrogels were expected to be candidates for 3D culture and 3D bioprinting [64].…”

Section: Synthetic Polymersmentioning

confidence: 99%

Bio-Inspired Hydrogels via 3D Bioprinting

Wang¹,

Deng²,

Shavandi³

2021

Biomimetics

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Many soft tissues of the human body such as cartilages, muscles, and ligaments are mainly composed of biological hydrogels possessing excellent mechanical properties and delicate structures. Nowadays, bio-inspired hydrogels have been intensively explored due to their promising potential applications in tissue engineering. However, the traditional manufacturing technology is challenging to produce the bio-inspired hydrogels, and the typical biological composite topologies of bio-inspired hydrogels are accessible completed using 3D bioprinting at micrometer resolution. In this chapter, the 3D bioprinting techniques used for the fabrication of bio-inspired hydrogels were summarized, and the materials used were outlined. This chapter also focuses on the applications of bio-inspired hydrogels fabricated using available 3D bioprinting technologies. The development of 3D bioprinting techniques in the future would bring us closer to the fabrication capabilities of living organisms, which would be widely used in biomedical applications.

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“…According to the source, hydrogel derives basically from nature and synthesized composition. Alginates, 45 collagen, 46 hyaluronic acid (HA), 47,48 gelatin, 49,50 chitosan, 51 cellulose, 50,52 and silk fibroin (SF) 53,52 are representative natural hydrogel materials, which are widely considered biocompatible for histogenesis, drug encapsulation, and tissue filling. Nevertheless, poor mechanical properties are the shortcomings hindering the wide application of those natural‐derived hydrogel 55 .…”

Section: The Classification Preparation Of Hydrogel and Its Advantamentioning

confidence: 99%

Advances and trends of hydrogel therapy platform in localized tumor treatment: A review

Tan

Huang

et al. 2020

J Biomedical Materials Res

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Due to limitations of treatment and the stubbornness of infiltrative tumor cells, the outcome of conventional antitumor treatment is often compromised by a variety of factors, including severe side effects, unexpected recurrence, and massive tissue loss during the treatment. Hydrogel‐based therapy is becoming a promising option of cancer treatment, because of its controllability, biocompatibility, high drug loading, prolonged drug release, and specific stimuli‐sensitivity. Hydrogel‐based therapy has good malleability and can reach some areas that cannot be easily touched by surgeons. Furthermore, hydrogel can be used not only as a carrier for tumor treatment agents, but also as a scaffold for tissue repair. In this review, we presented the latest researches in hydrogel applications of localized tumor therapy and highlighted the recent progress of hydrogel‐based therapy in preventing postoperative tumor recurrence and improving tissue repair, thus proposing a new trend of hydrogel‐based technology in localized tumor therapy. And this review aims to provide a novel reference and inspire thoughts for a more accurate and individualized cancer treatment.

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Fabrication of versatile dynamic hyaluronic acid-based hydrogels

Cited by 110 publications

References 78 publications

Additive-lathe 3D bioprinting of bilayered nerve conduits incorporated with supportive cells

Additive-lathe 3D bioprinting of bilayered nerve conduits incorporated with supportive cells

Bio-Inspired Hydrogels via 3D Bioprinting

Advances and trends of hydrogel therapy platform in localized tumor treatment: A review

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