Facile approach to the synthesis of molecularly imprinted ratiometric fluorescence nanosensor for the visual detection of folic acid

Abstract

A core-shell molecular imprinting fluorescence nanosensor was developed for the ratiometric fluorescence and visual detection of folic acid (FA). The nanosensor was prepared by anchoring imprinting shell on the silica nanoparticles, and embedding the CdTe quantum dots in imprinted shell to provide FA-dependent fluorescence signals. Under the optimum conditions, a favorable linearity relationship between the fluorescence intensities ratio (I 449 /I 619 ) and the FA concentration over 0.23-113 μM was offered with a detection limit (LOD) of 48 nM. The visual detection for FA was realized by evaluating profuse fluorescence color change from red to pink to purple to final blue. The proposed sensor possessed excellent sensing performances of rapid response, high precision, super sensitivity and selective recognition. Furthermore, endogenous FA was detected in real samples ranging from 37.4 to 265.8 μg/100 g; satisfactory spiked recoveries were obtained within 94.8-104.2%, which conformed to the measurement results by HPLC-UV.

Molecularly Imprinted Polymer-Quantum Dot Materials in Optical Sensors: An Overview of Their Synthesis and Applications

“…Thus, the proposed test strips could be applied to facile and fast daily monitoring of dopamine in serum samples without expensive instruments. Signal variation of the ratiometric fluorescent probe can be observed by the naked eye which makes the sensor suitable for semi-quantitative determinations in medical fields such as point-of-care testing and daily monitoring [27,33,53,54]. This was nicely demonstrated by Wang et al in the molecular imprinting based hybrid ratiometric fluorescence sensor for visual determination of bovine hemoglobin (BHb) [56].…”

“…Due to the already mentioned intrinsic self-calibration, environmental disturbances can be avoided providing accuracy and higher sensitivity to trace analytes. Accordingly, in the last few years an increasing number of molecularly imprinted ratiometric fluorescence nanosensors have been proposed for the determination of various target analytes, such as malachite green in river and lake waters [34], folic acid in serum and food [53,54], sinapic acid in seeds [33], and dopamine [55] among others.…”

Determination of folic acid by capillary zone electrophoresis with indirect chemiluminescence detection

“…In recent years, several methods employed for FA determination have been reported, including molecular imprinting sensor, 4,5 high-performance liquid chromatography (HPLC), 6–9 capillary electrophoresis, 10,11 flow-injection analysis (FIA), 12,13 enzyme-linked immunosorbent assay (ELISA), 14 chemiluminescence (CL) 15 and spectrophotometry. 16–19 Compared with conventional HPLC and ELISA methods, capillary electrophoresis (CE) is characterized by higher resolution and fewer samples and is a powerful tool in clinical diagnosis, as well as environmental and food analyses.…”

Ratiometric Fluorescent Probe Based on Diazotization-Coupling Reaction for Determination of Clenbuterol

“…The emergence of ratiometric fluorescence analysis technology has brought a new hope to solve the above drawbacks. It can efficiently minimize interference and improve sensitivity based on the built-in calibration through the ratio of two fluorescence signals at different wavelengths. , Also, the successive color changes under a UV lamp can be used for semiquantitative visualization analysis, which holds promise for the development of portable fluorescence equipment. , However, as far as we know, there is no report on ratiometric fluorescent probes used for CLB detection.…”

“…24,25 Also, the successive color changes under a UV lamp can be used for semiquantitative visualization analysis, which holds promise for the development of portable fluorescence equipment. 23,26 However, as far as we know, there is no report on ratiometric fluorescent probes used for CLB detection.…”