Facile Cell Sheet Harvest and Translocation Mediated by a Thermally Expandable Hydrogel with Controlled Cell Adhesion

Lee, Yu Bin; Shin, Young Min; Kim, Eun Mi; Lim, Jaeman; Lee, Joong-Yup; Shin, Heungsoo

doi:10.1002/adhm.201600210

Cited by 13 publications

(7 citation statements)

References 33 publications

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“…Another popular physical strategy is thermally mediated sol-gel transition. [22][23][24][25] For instance, gelatin solutions are emulsified at 40 °C, and the droplets are subsequently cooled down to 5 °C to induce gelation. 26 The advantage of this method lies in its biocompatibility because it doesn't require any toxic reagents.…”

Section: Kam W Leongmentioning

confidence: 99%

Cell-laden microfluidic microgels for tissue regeneration

et al. 2016

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Regenerating the diseased tissue is one of the foremost concerns for the millions of patients who suffer from tissue damage each year. Local delivery of cell-laden hydrogels offers an attractive approach for tissue repair. However, due to the typical macroscopic size of these cell constructs, the encapsulated cells often suffer from poor nutrient exchange. These issues can be mitigated by incorporating cells into microscopic hydrogels, or microgels, whose large surface-to-volume ratio promotes efficient mass transport and enhanced cell-matrix interactions. Using microfluidic technology, monodisperse cell-laden microgels with tunable sizes can be generated in a high-throughput manner, making them useful building blocks that can be assembled into tissue constructs with spatially controlled physicochemical properties. In this review, we examine microfluidics-generated cell-laden microgels for tissue regeneration applications. We provide a brief overview of the common biomaterials, gelation mechanisms, and microfluidic device designs that are used to generate these microgels, and summarize the most recent works on how they are applied to tissue regeneration. Finally, we discuss future applications of microfluidic cell-laden microgels as well as existing challenges that should be resolved to stimulate their clinical application.

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Section: Kam W Leongmentioning

confidence: 99%

Cell-laden microfluidic microgels for tissue regeneration

et al. 2016

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“…Recently, a method using thermosensitive Tetronic ® -based hydrogels has been reported, which can detach various cell sheets at the same time as the size expansion caused by dropping the temperature below 37 °C. This method took less than 10 min at 4 °C or more than 15 min at 25 °C [33,34].…”

Section: Cell Sheet Harvesting Methodsmentioning

confidence: 99%

Recent Advances in ROS-Responsive Cell Sheet Techniques for Tissue Engineering

Koo

Lee

Park

2019

IJMS

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Cell sheet engineering has evolved rapidly in recent years as a new approach for cell-based therapy. Cell sheet harvest technology is important for producing viable, transplantable cell sheets and applying them to tissue engineering. To date, most cell sheet studies use thermo-responsive systems to detach cell sheets. However, other approaches have been reported. This review provides the progress in cell sheet detachment techniques, particularly reactive oxygen species (ROS)-responsive strategies. Therefore, we present a comprehensive introduction to ROS, their application in regenerative medicine, and considerations on how to use ROS in cell detachment. The review also discusses current limitations and challenges for clarifying the mechanism of the ROS-responsive cell sheet detachment.

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“…More recently, some groups have leveraged thermosensitive Tetronic ® -based hydrogels to detach multiple cell sheets in response to size expansion induced by temperature decreases below 37°C, requiring over 15 min at 25°C but under 10 min at 4°C. 37,38 It has also been investigated to manipulate cell sheets in a two-dimensional (2D) format using temperature-sensitive culture plates, with supporting membranes used for cell sheet harvesting often consisting of porous poly (ethylene terephthalate), 39 and hydrophilically-modified poly (vinylidene difluoride). 40 To facilitate 3D tissue development, plunger-based devices have been designed to aid in the process of cell sheet manipulation.…”

Section: Temperature-responsive Systemsmentioning

confidence: 99%

Section: Preparation Of Cell Sheetsmentioning

confidence: 99%

Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration

You¹,

Lu²,

Li³

et al. 2022

IJN

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Bone defects that are congenital or the result of infection, malignancy, or trauma represent a challenge to the global healthcare system. To address this issue, multiple research groups have been developing novel cell sheet technology (CST)-based approaches to promote bone regeneration. These methods hold promise for use in regenerative medicine because they preserve cellcell contacts, cell-extracellular matrix interactions, and the protein makeup of cell membranes. This review introduces the concept and preparation system of the cell sheet (CS), explores the application of CST in bone regeneration, highlights the current states of the bone regeneration via CST, and offers perspectives on the challenges and future research direction of translating current knowledge from the lab to the clinic.

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Facile Cell Sheet Harvest and Translocation Mediated by a Thermally Expandable Hydrogel with Controlled Cell Adhesion

Cited by 13 publications

References 33 publications

Cell-laden microfluidic microgels for tissue regeneration

Cell-laden microfluidic microgels for tissue regeneration

Recent Advances in ROS-Responsive Cell Sheet Techniques for Tissue Engineering

Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration

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