Facile chemoenzymic preparation of enantiomerically pure 2-methylglycerol derivatives as versatile trifunctional C4-synthons

Wirz, Beat; Barner, R.; Huebscher, J.

doi:10.1021/jo00067a034

Cited by 34 publications

(26 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dihydroxylation of 1 was performed by treatment with H 2 O 2 in H 2 O in the presence of a catalytic amount of H 2 WO 4 [8] (Scheme 2). 2-Methylglycerol (2) was used as the key intermediate for the synthesis of triesters 3 a±e.…”

Section: Resultsmentioning

confidence: 99%

Sterically Hindered Triacylglycerol Analogues as Potent Inhibitors of Human Digestive Lipases

Constantinou-Kokotou

Magrioti

Verger

2004

Chemistry A European J

View full text Add to dashboard Cite

A novel class of inhibitors of human digestive lipases have been developed. Various sterically hindered triacylglycerols based on 2-methyl- and 2-butylglycerol, and/or 2-methyl fatty acids were synthesized. The triacylglycerol analogues were tested for their ability to form stable monomolecular films at the air/water interface by recording their surface-pressure/molecular-area compression isotherms. The inhibition of human pancreatic and gastric lipases by the sterically hindered triacylglycerol analogues was studied by using the monolayer technique with mixed films of 1,2-dicaprin, which contained variable proportions of each inhibitor. Triolein analogues that contain a butyl group at the 2-position of the glycerol backbone or methyl groups both at the 2-position of glycerol, and the alpha-position of each oleic acid residue were potent inhibitors; this caused a 50% decrease in HPL activity at 0.003 molar fraction.

show abstract

Section: Resultsmentioning

confidence: 99%

Sterically Hindered Triacylglycerol Analogues as Potent Inhibitors of Human Digestive Lipases

Constantinou-Kokotou

Magrioti

Verger

2004

Chemistry A European J

View full text Add to dashboard Cite

show abstract

“…This polymer has been successfully used for the immobilisation of lipase by several authors and the protocol of preparation was found to allow a better stability than the ones used with other supports [3,23,24]. Good results were also obtained in a number of previous studies for the immobilisation of other hydrolases such ␣-and ␤-galactosidase [25,26], pepsin, trypsin [18], penicillin G acylase [27].…”

Section: Introductionmentioning

confidence: 92%

Immobilisation of horseradish peroxidase on Eupergit®C for the enzymatic elimination of phenol

Pramparo

Stüber

Font

et al. 2010

Journal of Hazardous Materials

View full text Add to dashboard Cite

“…The presence of epoxy groups in Eupergit® provides a binding site for enzymes (32,33,34). Since the sugar residues in glycoenzymes are often not required for their activities, a novel more efficient strategy, which includes binding to polymers via activated carbohydrate moieties, seems to be very promising (33,34).…”

Section: Introductionmentioning

confidence: 99%

Real Time Diagnostic Point of Care by Amperometric Immuno-Biosensor Kit by Flow Technology

Braustein

2014

ECS Trans.

View full text Add to dashboard Cite

There is a growing need for virus-detecting sensors with improved sensitivity and dynamic range, for applications including disease diagnosis, pharmaceutical research, agriculture and homeland security. We report a novel electrochemical biosensing method for improving the sensitivity for detection of the bacteriophage virus MS2, using nanoporous oxirane-derivatized beads. These beads are a commercial polymethyl-metacrylate (PMMA) polymer that has extremely high surface area to volume ratio, making it an ideal platform for surface based sensors. We have developed and evaluated a method for covalent bioconjugation of antibodies and biological support to polymeric beads. The resulting Solid State Kits (SSK) were used to selectively capture enzyme-labeled MS2 viruses from different solutions, enabling detection of a viral concentration of as low as 10 plaque-forming units per milliliter (pfu ml -1 ) by measuring the current (A) from the exposed SSK beads to the enzymatic reaction electrons movement not clear. The kit is connected to a "home made" designed micro-flow system, that exhibits sensitivity and dynamic range similar to the ELISA immuno-liquid array-based assay while outperforming protein micro-array methods.Immuno-Amperometric techniques, using nano-Bio-Polymers Solid Phase Disposable Kit, were used to measure and thus to validate the accuracy of novel technology for virus concentration determination. These work demonstrate the utility of immunoelectrochemical techniques for use in environmental-health quality assurance measurements of viruses.

show abstract

Facile chemoenzymic preparation of enantiomerically pure 2-methylglycerol derivatives as versatile trifunctional C4-synthons

Cited by 34 publications

References 0 publications

Sterically Hindered Triacylglycerol Analogues as Potent Inhibitors of Human Digestive Lipases

Sterically Hindered Triacylglycerol Analogues as Potent Inhibitors of Human Digestive Lipases

Immobilisation of horseradish peroxidase on Eupergit®C for the enzymatic elimination of phenol

Real Time Diagnostic Point of Care by Amperometric Immuno-Biosensor Kit by Flow Technology

Contact Info

Product

Resources

About