Facile controlling internal structure of β-carotene-loaded protein nanoparticles by Flash Nanoprecipitation

Bhutto, Rizwan Ahmed; Fu, Zhinan; Wang, Mingwei; Yu, Jianxing; Zhao, Fang; Khanal, Santosh; Halepoto, Adeel; Wang, Junyou; Stuart, Martien A. Cohen; Guo, Xuhong

doi:10.1016/j.matlet.2021.130523

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…The mixing time is a function of the flow rate. The mixing time (τ mix ) was not short enough to provide a homogeneous micromixing environment at a lower flow rate, resulting in the larger size and broad size distribution of NPs. ,,,, In a word, a higher flow rate enhances the mixing of the polyanion and polycation and shortens the mixing time, increasing the rate of energy dissipation, thus reducing the size of the particles and narrowing the size distribution. RFNP can prevent growth and aggregation faster, thus forming relatively stable NCs. , These results are consistent with those previously reported.…”

Section: Resultsmentioning

confidence: 99%

Control and Preparation of Quaternized Chitosan and Carboxymethyl Chitosan Nanoscale Polyelectrolyte Complexes Based on Reactive Flash Nanoprecipitation

Bhutto

Hira

et al. 2021

ACS Omega

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Nanoscale polyelectrolyte complex materials have been extensively investigated for their promising application in protocell, drug carriers, imaging, and catalysis. However, the conventional preparation approach involving positive and negative polyelectrolytes leads to large size, wide size distribution, instability, and aggregation due to the nonhomogeneous mixing process. Herein, we employ reactive flash nanoprecipitation (RFNP) to control the mixing and preparation of the nanoscale polyelectrolyte complex. With RFNP, homogeneous mixing complexation between oppositely charged chitosan derivatives could be achieved, resulting in stable nanoscale complexes (NCs) with controllable size and narrow size distribution. The smallest size of NCs is found at specific pH due to the maximum attraction of positive and negative molecules of chitosan. The size can be modulated by altering the volumetric flow rates of inlet streams, concentration, and charge molar ratio of two oppositely charged chitosan derivatives. The charge molar ratio is also tuned to create NCs with positive and negative shells. There is no significant variation in the size of NCs produced at different intervals of time. This method allows continuous and tunable NC production and could have the potential for fast, practical translation.

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Section: Resultsmentioning

confidence: 99%

Control and Preparation of Quaternized Chitosan and Carboxymethyl Chitosan Nanoscale Polyelectrolyte Complexes Based on Reactive Flash Nanoprecipitation

Bhutto

Hira

et al. 2021

ACS Omega

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“…The nanoprecipitation method prepares nanoparticles by controlled mixing of solute solution and non-solvent with rapid reaction and is suitable for the preparation of a wide range of nanoparticles. Ahmed ( 75 ) used FNP to encapsulate β-carotene in NPs via a food-grade protein (sodium caseinate). FNP showed significant advantages in controlling NP size and increasing β-carotene loading and stability.…”

Section: Construction Of Nano Slow-release Systemmentioning

confidence: 99%

Construction of nano slow-release systems for antibacterial active substances and its applications: A comprehensive review

et al. 2023

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At present, nano-carrier materials with antibacterial activity are of great significance. Due to the widespread resistance of many pathogenic microorganisms, it has seriously threatened human health. The natural antimicrobial substances extracted from fruits and vegetables can significantly improve their stability combined with nano-carrier materials. The resistance of pathogenic microorganisms will be substantially reduced, greatly enhancing the effect of active antimicrobial substances. Nanotechnology has excellent research prospects in the food industry, antibacterial preservation, food additives, food packaging, and other fields. This paper introduces nano-carrier materials and preparation techniques for loading and encapsulating active antibacterial substances in detail by constructing a nano-release system for active antibacterial substances. The antibacterial effect can be achieved by protecting them from adverse external conditions and destroying the membrane of pathogenic microorganisms. The mechanism of the slow release of the bacteriostatic active substance is also described. The mechanism of carrier loading and release is mainly through non-covalent forces between the bacteriostatic active substance and the carrier material, such as hydrogen bonding, π-π stacking, van der Waals forces, electrostatic interactions, etc., as well as the loading and adsorption of the bacteriostatic active substance by the chemical assembly. Finally, its wide application in food and medicine is introduced. It is hoped to provide a theoretical basis and technical support for the efficient utilization and product development of bacteriostatic active substances.

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Potato protein as an emerging high-quality: Source, extraction, purification, properties (functional, nutritional, physicochemical, and processing), applications, and challenges using potato protein

Bhutto,

Khanal

et al. 2024

Food Hydrocolloids

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Facile controlling internal structure of β-carotene-loaded protein nanoparticles by Flash Nanoprecipitation

Cited by 5 publications

References 7 publications

Control and Preparation of Quaternized Chitosan and Carboxymethyl Chitosan Nanoscale Polyelectrolyte Complexes Based on Reactive Flash Nanoprecipitation

Control and Preparation of Quaternized Chitosan and Carboxymethyl Chitosan Nanoscale Polyelectrolyte Complexes Based on Reactive Flash Nanoprecipitation

Construction of nano slow-release systems for antibacterial active substances and its applications: A comprehensive review

Potato protein as an emerging high-quality: Source, extraction, purification, properties (functional, nutritional, physicochemical, and processing), applications, and challenges using potato protein

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