Facile Coupling of Droplet Magnetofluidic-Enabled Automated Sample Preparation for Digital Nucleic Acid Amplification Testing and Analysis

Abstract

Detailed description of the automated magnetofluidic sample purification and extraction routine; evaluation of the Amplitaq Gold polymerase with homemade buffers of various pH values; calculation of the extraction efficiency; digital high-resolution melt analysis in the Nanoarray; and single-plex digital melt curves for S. Aureus and E. coli (PDF)

Facile Filtration-Based Workflow for Facilitating Sensitive Detection of Pathogenic Bacteria from Blood

“…Third, we combined our blood processing workflow with benchtop PCR as an initial demonstration. We envision appending other detection methods such as rapid, magnetofluidic-enabled PCR [33][34][35] to achieve fast turnaround, or digital PCR-HRM [36] to achieve polymicrobial detection. Finally, plans for antimicrobial susceptibility testing (AST) represent a crucial step toward comprehensive clinical utility.…”

Micro- and nanosystems for the detection of hemorrhagic fever viruses

“…112 Recently, E. Gaddes further integrated the steps of nucleic acid extraction and purification with digital PCR by automating the movement of magnetic beads between droplets. 113 With this method, bulk-assay detection can be greatly simplified and may be used for POC HFV detection in the future. In addition, the lack of quantitative readouts is an issue for many POC isothermal amplification methods.…”

Bridging the gap between development of point-of-care nucleic acid testing and patient care for sexually transmitted infections

“…132 Gaddes and colleagues reported on the development of a palm-sized droplet magnetofluidic-based nucleic acid extraction and purification instrument. 133 Xu and colleagues implemented this idea within a capillary with pre-filled lysis buffer and magnetic beads, and used a moving magnet to transport DNA released from lysed CT and NG and detection by LAMP. 134 Chen and colleagues reviewed advances in using magnetic particles actuated by magnetic fields for achieving integrated nucleic acid purification, amplification, and detection without manual operation or sophisticated microfluidic pumps and valves.…”