Facile engineered polymeric microdevice via co-coupling of phenylboronic acid and Protein A for oriented antibody immobilization enables substantial signal enhancement for an enhanced fluorescence immunoassay

Chang, Junbiao; Gao, Nailong; Dai, Peng; Zhu, Ziming; You, Hui; Han, Wei; Li, Lu

doi:10.1016/j.snb.2021.130444

Cited by 16 publications

(6 citation statements)

References 36 publications

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“…Because of its simple operation, fast response and high stability in practical detection, fluorescence immunosensor analysis is one of the most promising immunoassays at present. 34 Organic dyes are often used as fluorescent probes for fluorescence immunosensors, such as calcitonin 35 and fluorescein isothiocyanate (FITC) due to their low cost and easy to obtain. 36 However, these organic fluorescent dyes have some defects, such as hydrophobicity, easy photobleaching degradation, short fluorescence lifetime, low sensitivity and so on.…”

Section: Immunosensormentioning

confidence: 99%

Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

et al. 2022

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Section: Immunosensormentioning

confidence: 99%

Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

et al. 2022

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“…This results in compact coiled structures of end-grafted PEG, which will transition to an equilibrium random coil as the ionic strength is reduced and thereby fill the gaps between the covalently attached molecules . Nevertheless, there are proteins with a high affinity for PEG, which will always limit its suitability for passivating surfaces in single-molecule experiments. − Other researchers have tried alternative strategies to passivate surfaces by the formation of a high-density brush using different functionalized polymers. − …”

Section: Introductionmentioning

confidence: 99%

“… 34 − 36 Other researchers have tried alternative strategies to passivate surfaces by the formation of a high-density brush using different functionalized polymers. 37 − 39 …”

Section: Introductionmentioning

confidence: 99%

Surface Passivation with a Perfluoroalkane Brush Improves the Precision of Single-Molecule Measurements

Bueno-Alejo

Vega

Chaplin

et al. 2022

ACS Appl. Mater. Interfaces

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Single-molecule imaging is invaluable for investigating the heterogeneous behavior and interactions of biological molecules. However, an impediment to precise sampling of single molecules is the irreversible adsorption of components onto the surfaces of cover glasses. This causes continuous changes in the concentrations of different molecules dissolved or suspended in the aqueous phase from the moment a sample is dispensed, which will shift, over time, the position of chemical equilibria between monomeric and multimeric components. Interferometric scattering microscopy (iSCAT) is a technique in the single-molecule toolkit that has the capability to detect unlabeled proteins and protein complexes both as they adsorb onto and desorb from a glass surface. Here, we examine the reversible and irreversible interactions between a number of different proteins and glass via analysis of the adsorption and desorption of protein at the single-molecule level. Furthermore, we present a method for surface passivation that virtually eliminates irreversible adsorption while still ensuring the residence time of molecules on surfaces is sufficient for detection of adsorption by iSCAT. By grafting high-density perfluoroalkane brushes on cover-glass surfaces, we observe approximately equal numbers of adsorption and desorption events for proteins at the measurement surface (±1%). The fluorous–aqueous interface also prevents the kinetic trapping of protein complexes and assists in establishing a thermodynamic equilibrium between monomeric and multimeric components. This surface passivation approach is valuable for in vitro single-molecule experiments using iSCAT microscopy because it allows for continuous monitoring of adsorption and desorption of protein without either a decline in detection events or a change in sample composition due to the irreversible binding of protein to surfaces.

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“…Therefore, it is very necessary to establish a rapid and sensitive method for the detection of AFP. In recent years, there have been many methods for detecting AFP—for example, electrochemiluminescence [ 2 , 3 ], fluorescence immunoassay [ 4 , 5 ], chemiluminescence immunoassay [ 6 ] and enzyme-linked immunoassay [ 7 ]. Electrochemical immunosensors have been widely used in the detection of AFP in recent years due to their high sensitivity, good selectivity and simple instrumentation, which have advantages in the detection of AFP [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Alpha-Fetoprotein Based on Black Phosphorus Nanosheets Modification with Iron Ions

Chen

Lin

et al. 2022

Micromachines

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Black phosphorus nanosheets (BPNSs) were synthesized with liquid exfoliation combined with the ultrasonic method and loaded with Fe3+ by simply mixing. The morphology, structure and electrochemical properties of the synthesized Fe3+/BPNSs were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry (CV), etc. The load of Fe3+ can improve the electrochemical performance of BPNSs and enhance the sensitivity of the detection. Additionally, Fe3+/BPNSs display good biocompatibility. In this study, immunosensors based on Fe3+/BPNSs were constructed to detect alpha-fetoprotein (AFP). The detection is due to the specific binding between the AFP antigen and antibody on the surface of the immunosensors, which can reduce the current response of Fe3+/BPNSs. The immunosensors have a good linear relationship in the range of 0.005 ng·mL−1 to 50 ng·mL−1, and the detection limit is 1.2 pg·mL−1. The results show that surface modification with metal ions is a simple and effective way to improve the electrochemical properties of BPNSs, which will broaden the prospects for the future application of BPNSs in the electrochemical field.

show abstract

Facile engineered polymeric microdevice via co-coupling of phenylboronic acid and Protein A for oriented antibody immobilization enables substantial signal enhancement for an enhanced fluorescence immunoassay

Cited by 16 publications

References 36 publications

Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

Surface Passivation with a Perfluoroalkane Brush Improves the Precision of Single-Molecule Measurements

Electrochemical Detection of Alpha-Fetoprotein Based on Black Phosphorus Nanosheets Modification with Iron Ions

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