Facile fabrication of tissue-engineered constructs using nanopatterned cell sheets and magnetic levitation

Abstract

We report a simple and versatile method for in vitro fabrication of scaffold-free tissue-engineered constructs with predetermined cellular alignment, by combining magnetic cell levitation with thermoresponsive nanofabricated substratum (TNFS) based cell sheet engineering technique. The TNFS based nanotopography provides contact guidance cues for regulation of cellular alignment and enables cell sheet transfer, while magnetic nanoparticles facilitate the magnetic levitation of the cell sheet. The temperature-mediated change in surface wettability of the thermoresponsive poly(N-isopropylacrylamide), PNIPAM, substratum enables the spontaneous detachment of cell monolayers, which can then be easily manipulated through use of a ring or disk shaped magnet. Our developed platform could be readily applicable to production of tissue-engineered constructs containing complex physiological structures for the study of tissue structure-function relationships, drug screening, and regenerative medicine.

Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues

“…To fabricate biologically functional cardiac tissues, myocardial cells were transformed into various patterns designed by the use of two-dimensional (2D) topography, resulting in three-dimensional (3D) cardiac tissue. [13][14][15][16][17][18][19][20][21][22][23] Although such 2D patterning facilitated the formation of cardiac tissue, the anisotropic alignment of CMs, which is quintessential for cardiac tissue, was hampered by a lack of 3D structural information. Therefore, a 3D matrix was utilized to overcome the limitation of the 2D scaffolds.…”

Adipose‑derived stem cell sheets combined with β‑tricalcium phosphate/collagen‑I fiber scaffold improve cell osteogenesis

“…A number of approaches to harvesting intact cell sheets have been previously reported, including temperature-responsive culture ( 29 ), magnetic force ( 30 ), electron beam irradiation ( 31 ) and vitamin C application ( 32 ). However, these mentioned approaches require complex procedures and novel materials, which may affect cell proliferation or differentiation.…”

“…The dispersed ADSCs were cultured under the same laboratory conditions and seeded on the β-TCP/COL-I scaffolds for comparison with the sheet protocol and further characterization of cell scaffold interactions. The β-TCP/COL-I scaffold combined with scattered ADSCs was fixed with 2.5% glutaraldehyde (Shanghai Pharmaceuticals) for 2 h, followed by serial dehydration for 15 min in a gradient of ethanol (30,50,70,85, 90 and 100%). Finally, the specimens were air-dried for 60 min and gold-sputtered for 60 sec at 10 A (E-1010; Hitachi, Ltd.).…”

Reversible Design of Dynamic Assemblies at Small Scales

“…[56] Magnetic levitation has been combined with nanopatterned cell sheets for fast and reversible interactions with their base structure to build thermoresponsive constructs for tissue engineering. [57] Furthermore, cell encapsulating microgel assemblies were generated via control of the magnetic field as well as tuning the paramagnetic medium (Figure 3a). [58] 3D scaffolds for bone repair in a clinically oriented application have also [40] Copyright 2019, AAAS.…”