Facile method for fabricating uniformly patterned and porous nanofibrous scaffolds for tissue engineering

Lim, Dong‐Jin; Sim, Myeongbu; Heo, Yunhoe; Jun, Ho−Wook; Park, Hansoo

doi:10.1007/s13233-015-3147-5

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…Bone injuries and defects caused by tumor resection, trauma, and congenital deformity remain a major challenge in clinical therapy [1]. Compared to present-day clinical therapies such as autografts and allografts [2], bone tissue engineering provides an alternative approach for bone reconstruction through the substitution of damaged tissues and has developed rapidly [3][4][5][6][7]. Over the past years, nanoscale substrates have attracted increasing attention in bone tissue engineering because they can affect cell performance, such as migration, proliferation, mitosis and lineage-specific differentiation, and extracellular matrix (ECM) deposition [8,9].…”

Section: Introductionmentioning

confidence: 99%

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

Hokmabad

Davaran

Aghazadeh

et al. 2018

Tissue Eng Regen Med

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BACKGROUND: The major challenge of tissue engineering is to develop constructions with suitable properties which would mimic the natural extracellular matrix to induce the proliferation and differentiation of cells. Poly(e-caprolactone)poly(ethylene glycol)-poly(e-caprolactone) (PCL-PEG-PCL, PCEC), chitosan (CS), nano-silica (n-SiO 2) and nano-hydroxyapatite (n-HA) are biomaterials successfully applied for the preparation of 3D structures appropriate for tissue engineering. METHODS: We evaluated the effect of n-HA and n-SiO 2 incorporated PCEC-CS nanofibers on physical properties and osteogenic differentiation of human dental pulp stem cells (hDPSCs). Fourier transform infrared spectroscopy, field emission scanning electron microscope, transmission electron microscope, thermogravimetric analysis, contact angle and mechanical test were applied to evaluate the physicochemical properties of nanofibers. Cell adhesion and proliferation of hDPSCs and their osteoblastic differentiation on nanofibers were assessed using MTT assay, DAPI staining, alizarin red S staining, and QRT-PCR assay. RESULTS: All the samples demonstrated bead-less morphologies with an average diameter in the range of 190-260 nm. The mechanical test studies showed that scaffolds incorporated with n-HA had a higher tensile strength than ones incorporated with n-SiO 2. While the hydrophilicity of n-SiO 2 incorporated PCEC-CS nanofibers was higher than that of samples enriched with n-HA. Cell adhesion and proliferation studies showed that n-HA incorporated nanofibers were slightly superior to n-SiO 2 incorporated ones. Alizarin red S staining and QRT-PCR analysis confirmed the osteogenic differentiation of hDPSCs on PCEC-CS nanofibers incorporated with n-HA and n-SiO 2. CONCLUSION: Compared to other groups, PCEC-CS nanofibers incorporated with 15 wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

Hokmabad

Davaran

Aghazadeh

et al. 2018

Tissue Eng Regen Med

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“…Nanofiber can recapitulate characteristics of native cellular microenvironments for in vitro cell cultures. Various cell types have been seeded onto electrospun fibers, with the results indicating that fibrous structures have a more favorable influence on cell growth than planar 2D surfaces do [ 124 , 125 , 126 , 127 , 128 ]. Although a lot of nanofiber-related studies have been reported, most of them involve control of the properties of either the nanofiber itself (nanometer scale) or of the macroscopic nanofiber matrix (millimeter or centimeter scale) [ 129 , 130 ].…”

Section: Cell Microarrays In a Biomimetic Environmentmentioning

confidence: 99%

Cell Microarray Technologies for High-Throughput Cell-Based Biosensors

Hong

Koom

Koh

2017

Sensors

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Due to the recent demand for high-throughput cellular assays, a lot of efforts have been made on miniaturization of cell-based biosensors by preparing cell microarrays. Various microfabrication technologies have been used to generate cell microarrays, where cells of different phenotypes are immobilized either on a flat substrate (positional array) or on particles (solution or suspension array) to achieve multiplexed and high-throughput cell-based biosensing. After introducing the fabrication methods for preparation of the positional and suspension cell microarrays, this review discusses the applications of the cell microarray including toxicology, drug discovery and detection of toxic agents.

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“…The basic science of electrospinning and several techniques enabling the efficient preparation of nanofibers were reported in the early part of this century. Electrospun nanofibers have been applied to various fields that can leverage their characteristic properties (high surface area, adjustable pore volume, and broad range of material types)567, such as tissue engineering89101112131415, sensors1617, drug delivery181920, wound dressing2122232425, and energy applications262728.…”

mentioning

confidence: 99%

Syringeless Electrospinning toward Versatile Fabrication of Nanofiber Web

Moon

Gil

Lee

2017

Sci Rep

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Although electrospinning is considered a powerful and generic tool for the preparation of nanofiber webs, several issues still need to be overcome for real-world applications. Most of these issues stem from the use of a syringe-based system, where the key factor influencing successful electrospinning is the maintenance of several subtle balances such as those of between the mass and the electrical state. It is extremely difficult to maintain these balances throughout the spinning process until all the polymeric solution in the syringe has been consumed. To overcome these limitations, we have developed a syringeless electrospinning technique as an alternative and efficient means of preparing a nanofiber web. This new technique uses a helically probed rotating cylinder. This technique can not only cover conventional methods, but also provides several advantages over syringe-based and needless electrospinning in terms of productivity (6 times higher) and processibility. For example, we can produce nanofibers with highly crystalline polymers and nanofiber-webs comprising networks of several different polymers, which is sometimes difficult in conventional electrospinning. In addition, this method provides several benefits for colloidal electrospinning as well. This method should help expand the range of applications for electrospun nanofiber webs in the near future.

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Facile method for fabricating uniformly patterned and porous nanofibrous scaffolds for tissue engineering

Cited by 6 publications

References 16 publications

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

Cell Microarray Technologies for High-Throughput Cell-Based Biosensors

Syringeless Electrospinning toward Versatile Fabrication of Nanofiber Web

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