The cellulose from bamboo has excellent toughness, hygroscopicity, and high crystallinity. Bamboo shoot dietary fiber can modulate the gut microbiota to prevent high-fat diet-induced obesity and can be applied for food fortification. Bamboo shoot contains a low content of lignin and is extracted easily for nanocellulose, which is used to prepare all kinds of composite materials. In this chapter, lignification process of bamboo shoot shells will first be discussed to reveal the principle of lignification. Then, the preparation methods and applications of cellulose, dietary fiber, and nanocellulose from bamboo shoots that were successively generalized to further improve the exploration and application of bamboo shoots or bamboo shoot wastes such as bamboo shoot shells.