Facile preparation of boronic acid‐functionalized magnetic nanoparticles with a high capacity and their use in the enrichment of cis‐diol‐containing compounds from plasma

Abstract

The use of bronate affinity adsorbents is a new separation method that appeared recently with great potential for specific extraction of cis-diol-containing compounds. In this work,a new strategy for the facile construction of boronic acid-functionalized Fe3 O4 magnetic nanoparticles (Fe3 O4 @FPBA MNPs) with a high capacity was described. The extraction capacity of the Fe3 O4 @FPBA MNPs was determined to be 66.0 ± 2.7 µmol/g for catechol and 80.6 ± 2.0 µmol/g for dopamine, being higher than that for the reported methods. The Fe3 O4 @FPBA MNPs were used to extract four cis-diol drugs: caffeic acid isopropyl ester, caffic acid bornyl ester, isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate and 3-(3, 4-dihydroxyphenyl)-2-hydroxylpropionic acid - from the spiked rabbit plasma, and the recoveries of four drugs were between 87.29 and104.37% with relative standard deviations ranging from 1.34 to 8.81%. Under the most favorable conditions, the solid-phase extraction combined with HPLC-UV for the analysis of four drugs in plasma could eliminate interferences from endogenous components of the biological fluids and exhibited sufficient precision and accuracy. These results showed that the prepared Fe3 O4 @FPBA MNPs were qualified for efficiently enriching and determining the trace cis-diol substances from biological samples.

Synthesis and application of boronic acid‐functionalized magnetic adsorbent for sensitive analysis of salbutamol residues in pig tissues

“…Nowadays, magnetic solid‐phase extraction (MSPE) is growing rapidly in popularity, because it has the characteristics of the large chemically modifiable surface area, significant purification effect, good biocompatibility, low consumption of solvents and no need for special laboratory equipment. The modified boronic acid magnetic materials combine the superiorities of boronic acid and magnetic materials, and have been utilized in catecholamine drugs (Jiang et al ., ) and plant‐based drugs (Du et al ., ). These drugs all belong to the 1,2‐ cis ‐diol class of drugs and can form five‐membered covalent cyclic esters with boronic acid groups.…”

One-pot preparation of boronic acid and PEG bi-functionalized silica particles for separation and purification of catecholamine from rat serum

“…adsorption than other boronic acid-functionalized adsorbents reported in the literature. 18 We can conclude from the adsorption kinetics and isotherm results that the PEGylation on the surface of boronic acid-functionalized materials did not show adverse effects on the adsorption rate and capacity of the materials, while the biocompatibility of materials was improved at the same time.…”

“…15 Recently, boronic acid-functionalized materials as the sorbents for the extraction of catecholamines have been successfully used in some research studies. [16][17][18] Boronic acid can form boronate ester with a cis-diol group under alkaline conditions. This complex formation is reversible.…”

Facile preparation of a high‐capacity boronate‐affinity adsorbent based on low‐cost commercial supports for selective enrichment of cis‐diol‐containing biomolecules

“…Figure (inset plot) shows the Langmuir fitting curve; the maximum adsorption capacity of PGMA@FPBA adsorbent was calculated to be 448 μmol/g (Table S2). Compared with other boronate‐affinity adsorbents (Table ), the PGMA@FPBA was prepared with only one step and exhibited a higher binding capacity than the boronate‐affinity materials, except for one boronate‐affinity material . Moreover, the adsorption equilibrium was achieved within 1 min, indicating faster adsorption kinetics than other reported boronate‐affinity materials.…”