Facile preparation of cancer cell membrane vehicle loaded with indocyanine green for effective photothermal therapy of cancer

One‐pot preparation of small lipid‐indocyanine green nanoparticles to induced intracellular oxidative/thermal stress damage for effective colorectal cancer therapy

“…The ROS generation of different samples was evaluated according to previous report [31]. The stability and haemolysis assay were conducted as previously reported [30].…”

“…The size of the nanoparticle was measured by size analyser (Omni 90Plus PALS, Brookhaven Instruments, USA) and transmission electron microscope (TEM, JEM‐1400 Plus, JEOL, Japan), respectively. The photothermal translation efficacy of free ICG, L‐ICG NPs in comparison with PBS was conducted according to previous report [30]. The ROS generation of different samples was evaluated according to previous report [31].…”

One‐pot synthesis chlorin e6 nano‐precipitation for colorectal cancer treatment Ce6 NPs for colorectal cancer treatment

“…The morphology of Ce6 NPs was observed by using a transmission electron microscope (TEM, JEM‐1200, JEOL, Japan). The ROS generation of free Ce6 and Ce6 NPs was determined using 3‐diphenylisobenzofuran (DPBF) as the probe, according to a previous report [ 37 ] DPBF was added into the solution‐free Ce6 and Ce6 NPs (final concentration: 10 μg/ml) and then irradiated by using a 680‐nm laser (0.2 W/cm 2 , 5 min); the changes in absorption at 413 nm was recorded every 30 s. The stability and haemolysis assays were conducted using previous reported protocol [ 38 ]. The size of Ce6 NPs in PBS (pH 7.4) and 10% mouse plasma was monitored for 48 h. 2% red blood cells from a rabbit were incubated with Ce6 NPs at different concentrations in the dark for 1 h. In the end, the cells were removed by centrifugation (1500 rpm, 10 min) and the absorption at 413 nm was assessed in comparison to cells treated with pure water.…”