Facile preparation of fluorescent water-soluble non-conjugated polymer dots and fabricating an acetylcholinesterase biosensor

Abstract

Illustration of the MnO2–NCPDs sensor system for the detection of AChE and the screening of their inhibitors.

Ultrasensitivity Detecting AChE through “Covalent Assembly” and Signal Amplification Strategic Approaches and Applied to Screen Its Inhibitor

“…There are a number of methods for detecting AChE, such as colorimetric methods, − electrochemical assay, high-efficiency chromatography, and fluorescence methods. − Among them, fluorescence detection has attracted attention and great prospects due to its simplicity, convenience, and high sensitivity. The most common fluorescence detection strategy for AChE now was coupling of AChE with thiocholine (TCh), where AChE was used to catalyze acetylthiocholine (ATCh) to generate TCh and then induce the reproductive behavior from organic small-molecule fluorescent probes , or advanced nanomaterials (such as metal nanomaterials, − carbon dots, , polymers, − aggregation-induced luminescent materials, − etc.). In spite of this, the coupling strategy still has problems such as low sensitivity and poor portability and thus directly affects the sensitive, quantitative detection of early diagnosis of related diseases.…”