Facile Preparation of Hydrophilic Dual Functional Magnetic Metal‐Organic Frameworks as a Platform for Proteomics Research

Abstract

Highly specific enrichment of glycopeptides or phosphopeptides from complex biological samples is crucial and necessary before mass spectrometry analysis. In this work, a hydrophilic dual‐functional magnetic metal‐organic framework was prepared via a facile strategy for both glycopeptides and phosphopeptides enrichment. Self‐assemble polymerization of dopamine on magnetic graphene was performed in basic solution at room temperature, which offered good hydrophilicity and compatibility, Zr4+ was easily adhered to the polydopamine, and MOF reaction with amino ligand was easily to perform (magG@PDA@UiO‐66‐NH2). The large surface area and good hydrophilicity make it an ideal material for proteomics research. It was applied for glycopeptide enrichment based on hydrophilic interaction, while immobilized metal ion affinity chromatography for phosphopeptides enrichment, both exhibiting excellent specificity (1:1000 for phosphopeptides, 1:200 for glycopeptides) and sensitivity (1 fmol for phosphopeptides, 10 fmol for glycopeptides). It also shows good performance in human serum and defatted milk analysis.

Bifunctional Magnetic Supramolecular-Organic Framework: A Nanoprobe for Simultaneous Enrichment of Glycosylated and Phosphorylated Peptides

“…The simultaneous recognition of glycosylated and phosphorylated proteins plays a significant role in several cellular activities but receives much less research interests. Only a few materials have been applied in this field, such as chitosan nanomaterial, magnetic titania, , titanium nanoprobe, magnetic graphene, polyhedral oligomeric silsesquioxane, metal–organic frameworks (MOFs), and their derivatives. − Among these materials, MOFs with combined properties of HILIC and IMAC/MOAC have attracted much attention due to their unique properties including intrinsic porosity, high surface area, and abundant binding sites. However, it is rather difficult to develop reliable and reproducible methods to achieve robust MOFs with tailored structures.…”

Advances in hydrophilic metal–organic frameworks for N-linked glycopeptide enrichment

“…Owing to the high stability of Zr-MOF and the strong coordination between Frontiers in Chemistry frontiersin.org phosphate groups and zirconium ions, no obvious signal decline was observed for glycopeptide detection in the eighth reuse cycle. Liu et al synthesized magG@PDA@UiO-66-NH 2 by replacing the Fe 3 O 4 in the above strategy into magnetic graphene nanosheets (Liu B et al, 2019). With the similar metal ion affinity and hydrophilic interaction, the composite exhibited excellent specificity for phosphopeptides and glycopeptides, as well as fine applicability for phosphopeptide enrichment from human serum and defatted milk digest.…”

Recent trends in sorbents for bioaffinity chromatography

“…MOFs, which are new inorganic-organic hybrid materials, have been widely used in many fields such as catalytic degradation, enzyme immobilization, drug delivery, gas storage, and separation [67]. In recent years, these materials have become attractive for use in proteomics research to enrich both glycopeptides and phosphopeptides with their high surface areas and porosity, good stability, adjustable pore size, and outer-surface modification properties [53,55,56,58,[68][69][70]. Their enrichment efficiency and selectivity properties are limited due to the fact that MOFs have only one metal ion and limited coordination regions.…”

“…In the last decade, there has been an increasing trend in the enrichment of biomarkers with IMAC in various biotechnological and biomedical applications before mass spectrometric analysis for their detection in biological samples [7, 9, 42–44, 47–50, 14,15,51–60]. IMAC has become an important technique in the purification of histidine‐tagged proteins and phosphoproteins in recent years.…”