Facile preparation of magnetic graphene double‐sided mesoporous composites for the selective enrichment and analysis of endogenous peptides

Abstract

In this work, magnetic graphene double-sided mesoporous nanocomposites (mag-graphene@mSiO₂) were synthesized by coating a layer of mesoporous silica materials on each side of magnetic grapheme. The surfactant (CTAB) mediated sol-gel coating was performed using tetraethyl orthosilicate as the silica source. The as-made magnetic graphene double-sided mesoporous silica composites were treated with high-temperature calcination to remove the hydroxyl on the surface. The novel double-sided materials possess high surface area (167.8 cm²/g) and large pore volume (0.2 cm³/g). The highly open pore structure presents uniform pore size (3.2 nm) and structural stability. The hydrophobic interior pore walls could ensure an efficient adsorption of target molecules through hydrophobic-hydrophobic interaction. At the same time, the magnetic Fe₃O₄ particles on both sides of the materials could simplify the process of enrichment, which plays an important role in the treatment of complex biological samples. The magnetic graphene double-sided nanocomposites were successfully applied to size-selective and specific enrichment of peptides in standard peptide mixtures, protein digest solutions, and human urine samples. Finally, the novel material was applied to selective enrichment of endogenous peptides in mouse brain tissue. The enriched endogenous peptides were then analyzed by LC-MS/MS, and 409 endogenous peptides were detected and identified. The results demonstrate that the as-made mag-graphene@mSiO₂ have powerful potential for peptidome research.

Functionalized mesoporous silica-coated magnetic graphene oxide by polyglycerol-g-polycaprolactone with pH-responsive behavior: Designed for targeted and controlled doxorubicin delivery

“…Also, it has theoretically high surface area of 2600 m 2 /g, biocompatibility, unique structure, and relatively low cost [1][2][3][4]. It is a good candidate to efficiently deliver water-insoluble cancer drugs [5,6], proteins [7], gene medicines [8,9] and bioimaging agents [10] into cells.…”

Magnetic graphene oxide mesoporous silica hybrid nanoparticles with dendritic pH sensitive moieties coated by PEGylated alginate-co-poly (acrylic acid) for targeted and controlled drug delivery purposes

“…Then magnetized GO (mag.GO) is transformed into magnetic double-sided mesoporous graphene oxide (mag.MGO) ( Fig. 1) [17,18].…”

Graphene and carbon nanodots in mesoporous materials: an interactive platform for functional applications

“…Besides energy storage and catalysis, the sandwich structures have shown other promising applications, such as in nanoproteomics [15,16]. The well defined porosity of mesoporous ordered materials is in fact a good tool for extraction of endogenous peptides and exclusion of larger molecules.…”