Facile solvothermal syntheses of isostructural lanthanide(III) formates: Photocatalytic, photoluminescent chemosensing properties, and proficient precursors for metal oxide nanoparticles

This study aims to characterize the microencapsulated type of human Adipose Tissue Mesenchymal Stem Cells (hAT-MSCs) and nanoparticles containing a conditioned medium of hAT-MSCs (CM-hATMSCs). The hAT-MSCs microencapsulation was measured the cells viability on 1st, 7th, and 14th days, by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethylphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, stability test was performed using magnetic stirrer at a speed of 300, 700, 1100, and 1200 rpm (revolutions per minutes) and applied on hAT-MSCs microencapsulation. The particle size of CM-hATMSCs nanoparticles were measured by particle size analyzer (PSA) and the growth factor levels namely Epidermal Growth Factor (EGF), Interleukin-6 (IL-6), Vascular Endothelial Growth Factor (VEGF), Insulin-Like Growth Factor-1 (IGF-1) were investigated using ELISA method. In the 14th day showed decreasing of cell viability percentage from 90 - 100% to 50% in both of 1.5×105 and 3×105 cells hAT-MSCs microencapsulation. CM-hATMSCs microencapsulation are quite stable and has a fairly good mechanical resistance when tested for stability using rotation at a speed of 1,200 rpm only damages 10 % of it. The CM-hATMSCs nanoparticles collected from hAT-MSCs growth medium in PSA test have an average particle size of 141 nm. Based on measurement of growth factor level, hAT-MSCs microencapsulation (3×105 cells) and CM-hATMSCs nanoparticles showed secretion of growth factors of EGF, IL-6, VEGF, and IGF-1 compared to positive controls. In summary, hAT-MSCs microencapsulation formulation using alginate showed good product based on the viability, stability, structure, and growth factor secretion ability. The characterization results showed that microencapsulated hAT-MSCs and CM-hATMSCs nanoparticles were successfully synthesized with the appropriate criteria. HIGHLIGHTS Stem cell therapy using Human ATMSCs (hAT-MSCs) has the potential to accelerate wound healing on the skin both directly and indirectly hMSC transplantation is affected by environment and inflammation which can trigger unwanted cell differentiation, so the solution is to use the nanoparticle method (CM-hATMSC) as a drug mobilization system hAT-MSCs and CM-hATMSCs display high secretion of growth factors namely EGF, IL-6, VEGF, and IGF-1 hAT-MSCs and CM-hATMSCs characterization was successfully synthesized according to the criteria GRAPHICAL ABSTRACT

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“…A total of 2 mL of CM-hATMSCs nanoparticle were analyzed using the Particle Size Analyzer (PSA) Beckman coulter tool [25].…”

Section: Particle Size Analyzer Of Cm-hatmscs Nanoparticlementioning

confidence: 99%

Characterization of Microencapsulation Human Adipose Tissue Mesenchymal Stem Cells (hAT-MSCs) and Nanoparticle of Conditioned Medium of AT-MSCs

Faried

Adam²,

Widowati³

et al. 2023

Trends Sci

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Ultrasonic assisted synthesis of Zn(II) 2D coordination polymer and 4-nitroaniline photoluminescence sensing manifestation through DFT studies

Riasat

Sharif

Khurshid

et al. 2022

Journal of Coordination Chemistry

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Facile solvothermal syntheses of isostructural lanthanide(III) formates: Photocatalytic, photoluminescent chemosensing properties, and proficient precursors for metal oxide nanoparticles

Cited by 2 publications

References 102 publications

Characterization of Microencapsulation Human Adipose Tissue Mesenchymal Stem Cells (hAT-MSCs) and Nanoparticle of Conditioned Medium of AT-MSCs

Characterization of Microencapsulation Human Adipose Tissue Mesenchymal Stem Cells (hAT-MSCs) and Nanoparticle of Conditioned Medium of AT-MSCs

Ultrasonic assisted synthesis of Zn(II) 2D coordination polymer and 4-nitroaniline photoluminescence sensing manifestation through DFT studies

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