Facile synthesis and antibacterial applications of cuprous oxide/bovine serum albumin hierarchical nanocomposite particles

Abstract

A facile method to prepare cuprous oxide/bovine serum albumin (Cu 2 O/BSA) hierarchical nanocomposite particles (NCPs) through imitating biomineralization is presented. BSA acted as the structure-directing agents, guiding the nucleation, growth and assembly of Cu(OH) 2 at binding sites to construct the hierarchical structure and then Cu 2 O/BSA NCPs were achieved. The synthesized nanocomposite with hierarchical structure exhibited excellent antibacterial performance. The products obtained were characterized with XRD, TEM, FTIR, EDX and XPS to investigate the formation mechanism of Cu 2 O/BSA hierarchical structure. Cu 2 O/BSA NCPs with size of 20-50 nm built up by several 6-7 nm Cu 2 O nanocrystals with BSA showed better antibacterial performance in the comparative experiments, which was attributed to the special structure and good biocompatibility of the Cu 2 O/BSA NCPs. And the possible mechanism was presented to explain the excellent antibacterial properties of Cu 2 O/BSA NCPs.

Role of capping agents in the application of nanoparticles in biomedicine and environmental remediation: recent trends and future prospects

“…BSA also acts as a structure-directing agent as well as controlling the assembly, nucleation, and growth of nanoparticles. Cuprous oxide (Cu 2 O) nanoparticles capped with BSA have been reported to produce a hierarchical structure emulating biomineralization via the facile method [ 36 ].…”

Growth of ZIF-8 Nanoparticles In Situ on Graphene Oxide Nanosheets: A Multifunctional Nanoplatform for Combined Ion-Interference and Photothermal Therapy

“…The intensity of diffraction peaks is relatively weak, meaning the formed ZIF-8 has low crystallinity . After the modification with BSA, BSArGO@ZIF-8 NSs presented only a weak diffraction peak (211) of ZIF-8 at around 2θ = 13° and the characteristic peak of BSA at around 22° (Figure d) . Furthermore, the FT-IR spectrum of rGO@ZIF-8 NSs presents the typical absorption peaks of ZIF-8 (Figure S3).…”

Zinc Phosphate Nanoparticles Produced in Saliva

“…The assumption that the proteins, within the saliva, temporarily stabilize zinc phosphate nanoparticles seems rational as it is well-known that proteins have previously been utilized as colloidal stabilizers in the preparation of inorganic nanoparticles. [18,24,25] Therein, proteins are frequently used in biotemplated nanoparticle synthesis. Most frequently used is BSA, which in our study is present in saliva at a concentration of 0.48 g L -1 .…”

“…More specifically, the samples contained α‐amylase, BSA, as well as mucin (highly glycosylated proteins). BSA, for instance, is known to possess several cation binding sites, which can be formed by aromatic amino acid residues, and is used in the templated synthesis of inorganic nanostructures , , . However, more precise statements regarding the participation of the proteins present in the saliva samples in the nanostructure formation cannot be made at this point due to the high complexity of the reaction media.…”

“…BSA, for instance, is known to possess several cation binding sites, which can be formed by aromatic amino acid residues, and is used in the templated synthesis of inorganic nanostructures. [18,24,25] However, more precise statements regarding the participation of the proteins present in the saliva samples in the nanostructure formation cannot be made at this point due to the high complexity of the reaction media. The structural parameters describing the primary particles aggregating changed over time: mean core/shell radii decreased significantly, from r 0 = 2.2 ± 0.2 nm to 1.7 ± 0.1 nm, during the first seven hours of incubation time between the collection of samples C and D, and remained the same within uncertainties during the following storage over two weeks yielding sample E. This indicates a likewise change of the thickness of the stabilizing shell, i.e.…”