Facile synthesis of magnetic metal organic frameworks for highly efficient proteolytic digestion used in mass spectrometry-based proteomics

Zhai, Rui; Yuan, Yifeng; Jiao, Fenglong; Hao, Feiran; Fang, Xiang; Zhang, Yangjun; Qian, Xiaohong

doi:10.1016/j.aca.2017.08.048

Cited by 35 publications

(16 citation statements)

References 47 publications

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“…This is typically simplified by the process of protein digestion in-solution using proteolytic enzymes such as trypsin. Trypsin (Try) is typically immobilized on a solid support including MNPs to make it more friendly for the mass spectrometric analysis [155] , [156] . A method of combining trypsin-immobilized MNPs and microwave-assisted protein digestion is reported to study the proteins of human lens tissue, which were identified by liquid chromatography and mass spectrometry [157] .…”

Section: Biomedical Applications Of Surface Coated Mnpsmentioning

confidence: 99%

Surface-coated magnetic nanostructured materials for robust bio-catalysis and biomedical applications-A review

Bilal

Iqbal

Adil

et al. 2022

Journal of Advanced Research

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Section: Biomedical Applications Of Surface Coated Mnpsmentioning

confidence: 99%

Surface-coated magnetic nanostructured materials for robust bio-catalysis and biomedical applications-A review

Bilal

Iqbal

Adil

et al. 2022

Journal of Advanced Research

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“…For example, metal-organic frameworks (MOFs) are very similar to CS in terms of crystallinity and the presence of nanopores for absorbing molecules. [25][26][27] Huang, Shih, and co-workers firstly reported 28 the use of MOFs for SALDI, and cage-type MIL-100(Fe) MOFs were used as the matrix in SALDI to detect nonpolar polycyclic aromatic hydrocarbons (PAHs) as shown in Fig. 7.…”

Section: Application As "Surface" In Msmentioning

confidence: 99%

Combined Analysis Based on a Crystalline Sponge Method

Ohara

Yamaguchi

2020

ANAL. SCI.

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The crystalline sponge (CS) method was developed as an X-ray crystallographic molecular structure analysis method that can be performed without the need for crystallization of the analyte. CS has strong molecular recognition properties and a highly flexible framework. The amount of analyte can be reduced to sub-milligram level.These features of the crystalline nano-space allow for the determination of the absolute structure of a trace analyte. In this review, we focus on the discovery of the CS method and its applications to biosynthetic products in combination with NMR spectroscopy.We also describe some examples of the CS method that is used mainly in combination with mass spectrometry (MS). Both approaches demonstrate the potential of microanalysis to determine the molecular structure of an unknown sample. Finally, we mention the use of a crystalline "nano-surface" rather than a crystalline nano-space in MS, which can detect small metabolites as well as post-translation biomolecules.

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“…With the proposition of bottom‐up strategy in early days, a mass of reports presented the immobilization of enzymes on nanomaterials that can accelerate the digestion rate and decrease autodigestion 5a,194. Recent researches mainly focused on the incorporation of enzymes into MOFs, only a few reported the immobilization of enzyme on silica nanomaterials . Additionally, nanomaterials can also serve as matrix for peptides detection by MS .…”

Section: Other Applications In Proteomicsmentioning

confidence: 99%

Nanomaterials in Proteomics

Sun

Shen

et al. 2019

Adv Funct Materials

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For a deep understanding of the human proteome, the first crucial step is always the effective separation of targeted proteins/peptides from complex samples. In mass spectrometry‐based proteomics, nanomaterials have emerged as a highly effective means to separate targeted proteins/peptides and increase their relative abundance, which is beneficial to conduct mass spectrometric analysis. In particular, nanomaterials with different specific functionalities have received great attention in ordinary proteomics, phosphoproteomics, and glycoproteomics, for which the easily recognizable characteristics of these proteomes take the primary responsibility, such as the hydrophobicity, phosphate groups, cis–diol structure, and hydrophilicity. This review mainly focuses on the recent design and preparation of nanomaterials with specific recognition ability, as well as their application in the aforementioned three types of proteomics.

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Facile synthesis of magnetic metal organic frameworks for highly efficient proteolytic digestion used in mass spectrometry-based proteomics

Cited by 35 publications

References 47 publications

Surface-coated magnetic nanostructured materials for robust bio-catalysis and biomedical applications-A review

Surface-coated magnetic nanostructured materials for robust bio-catalysis and biomedical applications-A review

Combined Analysis Based on a Crystalline Sponge Method

Nanomaterials in Proteomics

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