Facile synthesis of silver nanoparticles with medicinal grass and its biological assessment

Balu, Satheeshkumar; Andra, Swetha; Kannan, Saranya; S, Manisha Vidyavathy; Muthalagu, Murugesan

doi:10.1016/j.matlet.2019.126900

Cited by 24 publications

(7 citation statements)

References 16 publications

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“…In contrast, after 48 h of incubation, the cell proliferation rate in lower concentrations increased above the control. This might be attributable to AgNPs achieving the optimal conditions after extending the incubation time, which increased the rate of cell growth [31]. Optical microscopic images (Figure 7) also revealed similar results, demonstrating that cell proliferation is visible after 48 h as compared to 24 h. Soares et al (2016) studied the size-dependent cytotoxicity of AgNPs using the sizes of 10 and 50 nm.…”

Section: Cell Viabilitymentioning

confidence: 73%

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Size-Dependent Antibacterial, Antidiabetic, and Toxicity of Silver Nanoparticles Synthesized Using Solvent Extraction of Rosa indica L. Petals

Balu

Andra²,

Damiri

et al. 2022

Pharmaceuticals

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In this study, silver nanoparticles (AgNPs) are synthesized through a green approach by employing Rosa indica L. petal (RE) extracts as reducing and stabilizing agents, which are extracted using three different solvents: ethanol (Et), acetone (Ac), and water (Aq). The phase formation of the AgNPs is confirmed using X-ray diffraction (XRD). Morphological analysis is performed using a field-emission scanning electron microscope (FESEM), which reveals that the AgNPs are spherical in shape. The size is estimated using ImageJ software, which is found to be ~12, 18, and 770 nm for RE-Ac-Ag, RE-Et-Ag, and RE-Aq-Ag, respectively. The phytochemicals of Rosa indica L. petals involved in the formation of the AgNPs are studied using Fourier transform infrared spectroscopy (FTIR). Finally, these materials are studied for their antibacterial, antidiabetic, antioxidant, and hemolytic activity, as well as cell toxicity properties. The materials, RE-Ac-Ag and RE-Et-Ag, are found to be more effective than RE-Aq-Ag in inhibiting E. coli (Gram-negative bacteria) and S. aureus (Gram-positive bacteria). Hemolytic studies reveal that all of the samples show concentration-dependent activity up to 50 µg/mL. RE-Ac-Ag and RE-Et-Ag exhibit nonhemolytic behavior, whereas RE-Aq-Ag remains nonhemolytic until 100 µg/mL. The antidiabetic ability of the AgNPs is evaluated using α-amylase inhibition assay (DNSA assay) and α-glucosidase inhibition assay. The results are found to be effective, with IC50 values of α-amylase and α-glycosidase being 50, 50, and 75 µg/mL for RE-Et-Ag, RE-Ac-Ag, and RE-Aq-Ag, respectively. DPPH assay shows that the AgNPs inhibited the antioxidants well, with IC50 values of 40 µg/mL for RE-Et-Ag and RE-Ac-Ag and 60 µg/mL for RE-Aq-Ag. The toxicity study reveals that the AgNPs show size- and concentration-dependent behavior. Overall, it is realized from the findings that RE-Ac-Ag, RE-Et-Ag, and RE-Aq-Ag show size-dependent antibacterial, antidiabetic, and toxicity properties.

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Section: Cell Viabilitymentioning

confidence: 73%

“…All the samples and controls were incubated for 3 h at 37 • C. The tubes were then centrifuged for 5 min at 2000 rpm. Finally, OD values were recorded at 545 nm [31]. To ensure reproducibility, all experiments were repeated three times.…”

Section: Hemolysis Assaymentioning

confidence: 99%

Size-Dependent Antibacterial, Antidiabetic, and Toxicity of Silver Nanoparticles Synthesized Using Solvent Extraction of Rosa indica L. Petals

Balu

Andra²,

Damiri

et al. 2022

Pharmaceuticals

Self Cite

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“…Green synthesis of silver nanoparticles (AgNPs) is an ecofriendly, cost-effective and promising approach for the discovery of novel therapeutics. Biologically synthesised AgNPs have been derived from various plants and microorganisms (Anandaradje et al, 2020;Balu et al, 2020). AgNPs have been synthesised from extracts from the leaves of plants such as Mimosa albida (Pilaquinga et al, 2020), the bark of Picea abies (Tanase et al, 2020), and the leaf of Pistacia atlantica (Hamelian et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera

2022

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Green synthesis of silver nanoparticles (AgNPs) is an ecofriendly, cost-effective and promising approach for discovery of novel therapeutics. The aim of the current work was to biogenic synthesize, characterize AgNPs using seed extracts of three economically important varieties of date palm (Iklas, Irziz and Shishi), and assess their anti-pathogenic bacterial activities. AgNPs were synthesised then characterised using electron microscopy and Fourier transform infrared analyses. The bactericidal activities of AgNPs against five different bacterial pathogens, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pneumoniae, were determined in vitro. In particular, changes in membrane integrity of virulent bacterial strains in response to AgNPs were investigated. Results of lactate dehydrogenase, alkaline phosphatase activity assays, and measurement of membrane potential revealed that the cytotoxic effects of the AgNPs were mainly centred on the plasma membrane of bacterial cells, leading to loss of its integrity and eventually cell death. In conclusion, green synthesis of AgNPs is an efficient, cost-effective and promising strategy to combat virulent antibiotic-resistant strains.

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“…The resistance to toxic heavy metals displayed by microorganisms, such as the bacteria Pseudomonas aeruginosa and the fungus Aspergillus niger , both used in chemical detoxification, inspired the development of greener routes to synthesise nanomaterials [ 3 , 4 ]. Nowadays, the use of hazardous chemicals and toxic reducing and stabilising agents in nanoparticle synthesis are gradually being replaced with more sustainable, safe and cost-effective synthesis routes [ 3 , 5 ]. As a result, these agents are being substituted by biological agents such as viruses, bacteria, fungi, yeasts, algae and plants or bio-extracted compounds such as proteins/peptides, carbohydrates and vitamins [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…AgNP research has grown rapidly over the years due to the optical, antimicrobial and anticancer properties of AgNPs, which make them extremely useful in the biomedicine and biotechnology field [ 4 , 5 , 6 , 7 , 23 , 24 ]. For example, the inhibitory growth effects of AgNPs have been reported against bacteria such as Pseudomonas aeruginosa , Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) [ 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH

Alves

Murray

2022

JoF

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Fungi’s ability to convert organic materials into bioactive products offers environmentally friendly solutions for diverse industries. In the nanotechnology field, fungi metabolites have been explored for green nanoparticle synthesis. Silver nanoparticle (AgNP) research has grown rapidly over recent years mainly due to the enhanced optical, antimicrobial and anticancer properties of AgNPs, which make them extremely useful in the biomedicine and biotechnology field. However, the biological synthesis mechanism is still not fully established. Therefore, this study aimed to evaluate the combined effect of time, temperature and pH variation in AgNP synthesis using three different fungi phyla (Ascomycota, Basidiomycota and Zygomycota) represented by six different fungi species: Cladophialophora bantiana (C. bantiana), Penicillium antarcticum (P. antarcticum), Trametes versicolor (T. versicolor), Trichoderma martiale (T. martiale), Umbelopsis isabellina (U. isabellina) and Bjerkandera adusta (B. adusta). Ultraviolet–visible (UV-Vis) spectrophotometry and transmission electron microscopy (TEM) results demonstrated the synthesis of AgNPs of different sizes (3 to 17 nm) and dispersity percentages (25 to 95%, within the same size range) using fungi extracts by changing physicochemical reaction parameters. It was observed that higher temperatures (90 °C) associated with basic pH (9 and 12) favoured the synthesis of monodisperse small AgNPs. Previous studies demonstrated enhanced antibacterial and anticancer properties correlated with smaller nanoparticle sizes. Therefore, the biologically synthesised AgNPs shown in this study have potential as sustainable substitutes for chemically made antibacterial and anticancer products. It was also shown that not all fungi species (B. adusta) secrete metabolites capable of reducing silver nitrate (AgNO3) precursors into AgNPs, demonstrating the importance of fungal screening studies.

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Facile synthesis of silver nanoparticles with medicinal grass and its biological assessment

Cited by 24 publications

References 16 publications

Size-Dependent Antibacterial, Antidiabetic, and Toxicity of Silver Nanoparticles Synthesized Using Solvent Extraction of Rosa indica L. Petals

Size-Dependent Antibacterial, Antidiabetic, and Toxicity of Silver Nanoparticles Synthesized Using Solvent Extraction of Rosa indica L. Petals

In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera

Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH

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