Facilitated Molecular Typing of Shigella Isolates Using ERIC-PCR

Kosek, Margaret; Yori, Pablo Peñataro; Gilman, Robert H.; Vela, Henry; Olórtegui, Maribel Paredes; Chávez, César Banda; Calderón, Maritza; Bao, Juan Perez; Hall, Eric R.; Maves, Ryan C; Burga, Rosa; Sánchez, Graciela Meza

doi:10.4269/ajtmh.2012.11-0671

Cited by 25 publications

(22 citation statements)

References 30 publications

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“…ERIC-PCR-based genotyping method is reproducible and as discriminative as pulsed field gel electrophoresis. It is fast, cheap, and requires no speJournal of Poultry Science,55 (1) cialized equipment or reagents (Bishi et al, 2008;Kosek et al, 2012). In this study, two Salmonella-positive overshoe samples collected from one person on farm A yielded an identical gel pattern, further demonstrating the reliability and accuracy of the ERIC-PCR.…”

Section: Eric-pcr Fingerprinting and Clustering Analysismentioning

confidence: 54%

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

Liu

et al. 2018

J. Poult. Sci.

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Salmonella is the most common cause of foodborne illnesses worldwide. Poultry eggs are a major contamination source of Salmonella. The prevalence of Salmonella has been effectively reduced since a series of measures were taken to reduce contamination in egg-laying houses. In the present study, 1,512 environmental samples obtained from layer farms of different production scales were screened in a voluntary Salmonella survey study. Contaminations were detected using a PCR method. Genetic relationships among Salmonella samples were specified using molecular typing by enterobacterial repetitive intergenic consensus (ERIC)-PCR. The survey results showed that two layer farms, located in the Shandong and Hebei provinces, were contaminated with Salmonella. Thirty-one samples from these two farms, including feed, drinking nipples, egg collection belt, air inlets and outlets, air, overshoes, and eggshells, were identified as Salmonella-positive. It was observed that certain samples within the henhouses as well as in the egg collecting areas showed relatively high genetic similarities. The survey conclusively revealed minor Salmonella contamination in northern China. Moreover, various areas within the layer farms were identified as part of the propagation chain of Salmonella. Furthermore, evidence of cross-contamination of Salmonella was found in the laying houses and egg collection areas, even between these two regions. Therefore, it is necessary to establish routine Salmonella detection and subsequent environmental control measures in order to decrease the prevalence of Salmonella.

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Section: Eric-pcr Fingerprinting and Clustering Analysismentioning

confidence: 54%

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

Liu

et al. 2018

J. Poult. Sci.

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“…However, PFGE is both time consuming and requires staff skilled in the method to be reproducible. The ERIC method, generally considered to be both reliable and sensitive, is a much simpler procedure than PFGE (Kosek et al, 2012;Fendri et al, 2013;Dorneles et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

ERIC-PCR genotyping of field isolates ofSalmonella entericasubsp.entericaserovar Gallinarum biovars Gallinarum and Pullorum

et al. 2015

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Salmonella Gallinarum (SG) and Salmonella Pullorum (SP) have been classified as biovars belonging to Salmonella enterica subsp. enterica serovar Gallinarum. Genetic diversity among isolates of the same biovar can be detected by DNA fingerprinting techniques which are useful in epidemiological investigations. In this study, we applied the PCR amplification of Enterobacterial Repetitive Intergenic Consensus sequences (ERIC-PCR) to analyse 45 strains of SG and SP, most of which were isolated from diseased poultry of different Brazilian regions over a period of 27 years until 2014. The ERIC-genotypes obtained were used to describe the epidemiological relationship amongst the strains. Our findings showed that there were six ERIC-patterns for SG strains at 80% similarity. In addition, some of the SG isolates recovered from different regions and years clustered with 100% similarity, suggesting that transfer of genotypes between these regions has taken place. The commercial rough vaccine strain 9R showed a unique profile. Meanwhile, more genetic diversity was observed among SP strains where ten ERICpatterns were also formed at 80% similarity.

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“…For Enterobacteriaceae, this ERIC-PCR assay is similar to PFGE in its ability to discriminate between related isolates and to group like isolates as similar [21], [22], [25]. 37°C curli-expressers were distributed across a range of ERIC types and include multiple pairs with less than the 85% similarity cutoff often used to distinguish strains (Figure 1B).…”

Section: Resultsmentioning

confidence: 99%

The Bacterial Amyloid Curli Is Associated with Urinary Source Bloodstream Infection

et al. 2014

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Urinary tract infections are the most common cause of E. coli bloodstream infections (BSI) but the mechanism of bloodstream invasion is poorly understood. Some clinical isolates have been observed to shield themselves with extracellular amyloid fibers called curli at physiologic temperature. We hypothesize that curli fiber assembly at 37°C promotes bacteremic progression by urinary E. coli strains. Curli expression by cultured E. coli isolates from bacteriuric patients in the presence and absence of bacteremia were compared using Western blotting following amyloid fiber disruption with hexafluoroisopropanol. At 37°C, urinary isolates from bacteremic patients were more likely to express curli than those from non-bacteremic patients [16/22 (73%) vs. 7/21 (33%); p = 0.01]. No significant difference in curli expression was observed at 30°C [86% (19/22) vs. 76% (16/21); p = 0.5]. Isolates were clonally diverse between patients, indicating that this phenotype is distributed across multiple lineages. Most same-patient urine and blood isolates were highly related, consistent with direct invasion of urinary bacteria into the bloodstream. 37°C curli expression was associated with bacteremic progression of urinary E. coli isolates in this population. These findings suggest new future diagnostic and virulence-targeting therapeutic approaches.

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Facilitated Molecular Typing of Shigella Isolates Using ERIC-PCR

Cited by 25 publications

References 30 publications

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

ERIC-PCR genotyping of field isolates ofSalmonella entericasubsp.entericaserovar Gallinarum biovars Gallinarum and Pullorum

The Bacterial Amyloid Curli Is Associated with Urinary Source Bloodstream Infection

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