The Bacterial Amyloid Curli Is Associated with Urinary Source Bloodstream Infection

Hung, Chia-Suei; Marschall, Jonas; Burnham, Carey-Ann D.; Byun, Albert S.; Henderson, Jeffrey P.

doi:10.1371/journal.pone.0086009

Cited by 35 publications

(35 citation statements)

References 37 publications

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“…Untreated UPEC infections can lead to dissemination and infections of the bloodstream, also known as bacteremia (136). Interestingly, UPEC strains isolated from bacteremic patients produce more curli at 37°C than UPEC strains isolated from non-bacteremic patients (137). Additionally, over 50% of E. coli isolates from patients with sepsis produce curli at 37°C (138).…”

Section: Enterobacteriaceae Extracellular Matrix In the Host And Diseasementioning

confidence: 99%

The Biology of the Escherichia coli Extracellular Matrix

2015

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Chapter Summary Escherichia coli (E. coli) is one of the world’s best-characterized organisms, as it has been extensively studied for over a century. However, most of this work has focused on E. coli grown under laboratory conditions that do not faithfully simulate its natural environments. Therefore, the historical perspectives on E. coli physiology and life cycle are somewhat skewed toward experimental systems that feature E. coli growing logarithmically in a test tube. Typically a commensal bacterium, E. coli resides in the lower intestines of a slew of animals. Outside of the lower intestine, E. coli can adapt and survive in a very different set of environmental conditions. Biofilm formation allows E. coli to survive, and even thrive, in environments that do not support the growth of planktonic populations. E. coli can form biofilms virtually everywhere; in the bladder during a urinary tract infection, on in-dwelling medical devices, and outside of the host on plants and in the soil. The E. coli extracellular matrix, primarily composed of the protein polymer named curli and the polysaccharide cellulose, promotes adherence to organic and inorganic surfaces, and resistance to desiccation, the host immune system and other antimicrobials. The pathways that govern E. coli biofilm formation, cellulose production, and curli biogenesis will be discussed in this book chapter, which concludes with insights into the future of E. coli biofilm research and potential therapies.

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Section: Enterobacteriaceae Extracellular Matrix In the Host And Diseasementioning

confidence: 99%

The Biology of the Escherichia coli Extracellular Matrix

2015

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“…Curli interact with many host proteins including serum and contact-phase proteins, which might promote bacterial dissemination and entrance into the bloodstream (summarised in Barnhart & Chapman, 2006). Curliated strains produced a more progressive acute infection in a mouse model (Kai-Larsen et al, 2010), and in concordance, curliated UPEC strains are more likely to cause urosepticaemia than non-curliated strains (Hung, Marschall, Burnham, Byun, & Henderson, 2014).…”

Section: Curli Fimbriaementioning

confidence: 99%

Virulence Factors of Uropathogenic E. coli and Their Interaction with the Host

Lüthje

Brauner

2014

Advances in Microbial Physiology

139

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“…Curli fimbriae are primarily composed of CsgA protein monomers and are associated with cystitis, pyelonephritis, and bacteremia (Norinder et al, 2012; Hung et al, 2014; Lim et al, 2014). These fimbriae are widely distributed in clinical strains of UPEC and are finely regulated by a regulatory system of on-to-off and/or off-to-on switching (Snyder et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Dimeric and Trimeric Fusion Proteins Generated with Fimbrial Adhesins of Uropathogenic Escherichia coli

Reyes‐Grajeda

Cruz‐Córdova

Saldaña-Ahuactzi

et al. 2016

Front. Cell. Infect. Microbiol.

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Urinary tract infections (UTIs) are associated with high rates of morbidity and mortality worldwide, and uropathogenic Escherichia coli (UPEC) is the main etiologic agent. Fimbriae assembled on the bacterial surface are essential for adhesion to the urinary tract epithelium. In this study, the FimH, CsgA, and PapG adhesins were fused to generate biomolecules for use as potential target vaccines against UTIs. The fusion protein design was generated using bioinformatics tools, and template fusion gene sequences were synthesized by GenScript in the following order fimH-csgA-papG-fimH-csgA (fcpfc) linked to the nucleotide sequence encoding the [EAAAK]5 peptide. Monomeric (fimH, csgA, and papG), dimeric (fimH-csgA), and trimeric (fimH-csgA-papG) genes were cloned into the pLATE31 expression vector and generated products of 1040, 539, 1139, 1442, and 2444 bp, respectively. Fusion protein expression in BL21 E. coli was induced with 1 mM IPTG, and His-tagged proteins were purified under denaturing conditions and refolded by dialysis using C-buffer. Coomassie blue-stained SDS-PAGE gels and Western blot analysis revealed bands of 29.5, 11.9, 33.9, 44.9, and 82.1 kDa, corresponding to FimH, CsgA, PapG, FC, and FCP proteins, respectively. Mass spectrometry analysis by MALDI-TOF/TOF revealed specific peptides that confirmed the fusion protein structures. Dynamic light scattering analysis revealed the polydispersed state of the fusion proteins. FimH, CsgA, and PapG stimulated the release of 372–398 pg/mL IL-6; interestingly, FC and FCP stimulated the release of 464.79 pg/mL (p ≤ 0.018) and 521.24 pg/mL (p ≤ 0.002) IL-6, respectively. In addition, FC and FCP stimulated the release of 398.52 pg/mL (p ≤ 0.001) and 450.40 pg/mL (p ≤ 0.002) IL-8, respectively. High levels of IgA and IgG antibodies in human sera reacted against the fusion proteins, and under identical conditions, low levels of IgA and IgG antibodies were detected in human urine. Rabbit polyclonal antibodies generated against FimH, CsgA, PapG, FC, and FCP blocked the adhesion of E. coli strain CFT073 to HTB5 bladder cells. In conclusion, the FC and FCP proteins were highly stable, demonstrated antigenic properties, and induced cytokine release (IL-6 and IL-8); furthermore, antibodies generated against these proteins showed protection against bacterial adhesion.

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The Bacterial Amyloid Curli Is Associated with Urinary Source Bloodstream Infection

Cited by 35 publications

References 37 publications

The Biology of the Escherichia coli Extracellular Matrix

The Biology of the Escherichia coli Extracellular Matrix

Virulence Factors of Uropathogenic E. coli and Their Interaction with the Host

Dimeric and Trimeric Fusion Proteins Generated with Fimbrial Adhesins of Uropathogenic Escherichia coli

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