Facilitated transport of CO<sub>2</sub> across a membrane bearing carbonic anhydrase

Broun, G.; Sélégny, Éric; Minh, Canh Tran; Thomas, Daniel

doi:10.1016/0014-5793(70)80166-1

Cited by 66 publications

(21 citation statements)

References 5 publications

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“…A role for CA in pH regulation has been suggested in other systems (36). A mitochondrial CA could also be involved in the diffusion and transport of inorganic carbon species within the mitochondrial matrix as well as their efflux, in analogy to what has been shown in other systems (3,37).…”

Section: Resultsmentioning

confidence: 74%

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Discovery of an algal mitochondrial carbonic anhydrase: molecular cloning and characterization of a low-CO2-induced polypeptide in Chlamydomonas reinhardtii.

Eriksson

Karlsson

Ramazanov

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

159

131

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Section: Resultsmentioning

confidence: 74%

“…It is involved in many different processes, such as ion exchange, acid-base balance, carboxylation/decarboxylation reactions, and inorganic carbon diffusion between the cell and its environment as well as within the cell (1)(2)(3)(4). CAs are found in animal and plant cells as well as in archea and eubacteria and are divided into three different families (5).…”

mentioning

confidence: 99%

Discovery of an algal mitochondrial carbonic anhydrase: molecular cloning and characterization of a low-CO2-induced polypeptide in Chlamydomonas reinhardtii.

Eriksson

Karlsson

Ramazanov

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

159

131

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“…A facilitated transport of carbon dioxide was shown to occur across a membrane bearing cocross-linked carbonic anhydrase [15]. An active transport of glucose was shown experimentally in a structured bienzymic model formed by sequential enzymes bound into proteineous artificial supports [16].…”

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confidence: 97%

Various vectorial behaviours of a spatially structured substrate cycle

Thiébart‐Fassy

Hervagault

1994

European Journal of Biochemistry

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The dynamic properties of the glucose/glucose-6-phosphate cycle are studied under conditions where the phosphatase and kinase interconverting enzymes are spatially distributed. A semi-artificial membrane made of compacted plant cell walls bearing active phosphatase in its natural state, separates two compartments, one of these compartments containing soluble hexokinase. Depending only upon the two enzyme activity levels and the initial distribution of the substrates, numerous asymmetrical and vectorial behaviours can be observed, such as facilitated glucose 6-phosphate diffusion, active transport of either glucose or glucose 6-phosphate and sequential (alternative) transport between glucose and glucose 6-phosphate. A diffusion-partition reaction coupling can account for these oriented mass transfers. The possibility of such a coupling in this model system is clearly dictated by the global analog of the Curie principle. These results may provide new insight on (a) the still obscure role played by the cell-wall phosphatase activities, particularly their involvement in the transport of exogenous phosphomonoesters, and (b) the actual in vivo operation of substrate and protein cycles in view of the heterogeneity and anisotropy of the cellular milieu.Cyclic processes are ubiquitous in metabolism. These processes are involved in many activities such as oxidation and synthesis, generation of energy and reducing equivalents. These cycles occur when two opposing metabolic pathways operate in which the reaction(s) in the forward and reverse directions are catalyzed by different enzymes. If the simultaneous operation of these two interconverting enzymes leads to the hydrolysis of an energy-rich compound (e.g. ATP, GTP) with no net flux of metabolites, such cycles are designated as futile. This latter situation is most frequently encountered in the phosphorylatioddephosphorylation cycles involving both low-molecular-mass metabolites (sugars, amino acids, nucleotides) and proteins, including enzymes (with covalent modifications).The role played by cycles (futile or not) in the regulation of metabolism is still a matter of controversy. However, from experimental data and theoretical considerations, it is likely that substrate (protein) cycling is involved in thermogenesis, the orientation of fluxes, the control of concentrations, and the amplification of sensitivity, particularly when organized in cascades [l-71. When dealing with cycles subjected to a destabilizing factor (product activation, substrate inhibition), more sophisticated dynamic behaviours may be observed, such as multistationarity (alternative steady states, reversible or irreversible), sustained oscillations or chaotic motions [8 -121.However, most of these cycles are implicitly supposed to operate under homogeneous and isotropic conditions. Many situations are known where either the enzyme or metabolite partners are spatially (heterogeneously) distributed between cellular compartments. Interconverting catalysts can also be located in or on membrane structures....

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“…A possible candidate in this context is the genetic introduction of carbonic anhydrase [EC 4. 2.1.1., CA] activity into mesophyll cytoplasm, since CA facilitates inorganic carbon diffusion through bilayer lipid membranes [1] and CA activity scarcely exists in contrast to its chloroplastic abundance in C3 plants [2] . Previously, Okabe observed that the introduction of liposome encapsulated bovine CA (Type II ) into tomato cotyledonous protoplasts by fusion technology (ca.…”

mentioning

confidence: 99%

“…We chose a mouse CA cDNA rather than one of plant origin for this study (1) protoplasts followed by the centrifugal fractionation of the chloroplasts. The chloroplastic and extrachloroplastic fractions were analyzed by SDS-PAGE and Western analysis.…”

mentioning

confidence: 99%

Photosynthetic carbon assimilation of transgenic tobacco plant expressing cytoplasmic carbonic anhydrase cDNA from mouse.

Jinushi

Okabe²,

Ishii

1998

Plant Biotechnology

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Facilitated transport of CO₂ across a membrane bearing carbonic anhydrase

Cited by 66 publications

References 5 publications

Discovery of an algal mitochondrial carbonic anhydrase: molecular cloning and characterization of a low-CO2-induced polypeptide in Chlamydomonas reinhardtii.

Discovery of an algal mitochondrial carbonic anhydrase: molecular cloning and characterization of a low-CO2-induced polypeptide in Chlamydomonas reinhardtii.

Various vectorial behaviours of a spatially structured substrate cycle

Photosynthetic carbon assimilation of transgenic tobacco plant expressing cytoplasmic carbonic anhydrase cDNA from mouse.

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Facilitated transport of CO2 across a membrane bearing carbonic anhydrase

Cited by 66 publications

References 5 publications

Discovery of an algal mitochondrial carbonic anhydrase: molecular cloning and characterization of a low-CO2-induced polypeptide in Chlamydomonas reinhardtii.

Discovery of an algal mitochondrial carbonic anhydrase: molecular cloning and characterization of a low-CO2-induced polypeptide in Chlamydomonas reinhardtii.

Various vectorial behaviours of a spatially structured substrate cycle

Photosynthetic carbon assimilation of transgenic tobacco plant expressing cytoplasmic carbonic anhydrase cDNA from mouse.

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Facilitated transport of CO₂ across a membrane bearing carbonic anhydrase