Facilitating viral vector movement enhances heterologous protein production in an established plant system

Wang, Xu; Prokhnevsky, Alexei I.; Skarjinskaia, Marina; Razzak, Md. Abdur; Streatfield, Stephen J.; Lee, Jung Youn

doi:10.1111/pbi.13977

Cited by 3 publications

(3 citation statements)

References 40 publications

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“…However, truncating the cytosolic tail results in a loss of protein accumulation in Nicotiana benthamiana and transgenic Arabidopsis ( A. thaliana ) plants ( Wang et al 2020 ). In a recent study, we identified a few amino acid residues in the cytosolic tail that are important for PDLP5 function, but not for localization ( Wang et al 2023 ). To investigate the role of the cytosolic tail in localization, we generated a variant of PDLP5 lacking the tail sequence, fused to enhanced green fluorescent protein (EGFP) (deletion cytosolic tail [ΔCT] in Fig.…”

Section: Resultsmentioning

confidence: 99%

Targeting of plasmodesmal proteins requires unconventional signals

Luna

Wang

et al. 2023

The Plant Cell

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Effective cellular signaling relies on precise spatial localization and dynamic interactions among proteins in specific subcellular compartments or niches, such as cell-to-cell contact sites and junctions. In plants, endogenous and pathogenic proteins gained the ability to target plasmodesmata, membrane-lined cytoplasmic connections, through evolution to regulate or exploit cellular signaling across cell wall boundaries. For example, the receptor-like membrane protein PLASMODESMATA-LOCATED PROTEIN 5 (PDLP5), a potent regulator of plasmodesmal permeability, generates feed-forward or feed-back signals important for plant immunity and root development. However, the molecular features that determine the plasmodesmal association of PDLP5 or other proteins remain largely unknown, and no protein motifs have been identified as plasmodesmal targeting signals. Here, we developed an approach combining custom-built machine-learning algorithms and targeted mutagenesis to examine PDLP5 in Arabidopsis thaliana and Nicotiana benthamiana. We report that PDLP5 and its closely related proteins carry unconventional targeting signals consisting of short stretches of amino acids. PDLP5 contains two divergent, tandemly arranged signals, either of which is sufficient for localization and biological function in regulating viral movement through plasmodesmata. Notably, plasmodesmal targeting signals exhibit little sequence conservation but are located similarly proximal to the membrane. These features appear to be a common theme in plasmodesmal targeting.

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Section: Resultsmentioning

confidence: 99%

Targeting of plasmodesmal proteins requires unconventional signals

Luna

Wang

et al. 2023

The Plant Cell

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“…The coding sequence was cloned into pCAMBIA-1302. The Arabidopsis overexpression lines were conducted and generated by A. tumefaciens GV3101 mediated transformation [ 92 ]. Three biological replicates were used, and the experiments were performed three times.…”

Section: Methodsmentioning

confidence: 99%

“…p2300-35s-H2B-mCherry was used as a nuclear marker. The plasmid was transferred into N. benthamiana leaves using A. tumefaciens GV3101-mediated transient infiltration [ 92 ]. Subcellular localization was observed using a laser scanning confocal microscope (Zeiss LSM900) with the wavelengths of 488 (excitation)/500 to 530 nm (emission) for GFP and 561 (excitation)/590 to 640 nm (emission) for mCherry.…”

Section: Methodsmentioning

confidence: 99%

Genomic insight into the origin, domestication, dispersal, diversification and human selection of Tartary buckwheat

He,

Zhang,

Shi

et al. 2024

Genome Biol

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Background Tartary buckwheat, Fagopyrum tataricum, is a pseudocereal crop with worldwide distribution and high nutritional value. However, the origin and domestication history of this crop remain to be elucidated. Results Here, by analyzing the population genomics of 567 accessions collected worldwide and reviewing historical documents, we find that Tartary buckwheat originated in the Himalayan region and then spread southwest possibly along with the migration of the Yi people, a minority in Southwestern China that has a long history of planting Tartary buckwheat. Along with the expansion of the Mongol Empire, Tartary buckwheat dispersed to Europe and ultimately to the rest of the world. The different natural growth environments resulted in adaptation, especially significant differences in salt tolerance between northern and southern Chinese Tartary buckwheat populations. By scanning for selective sweeps and using a genome-wide association study, we identify genes responsible for Tartary buckwheat domestication and differentiation, which we then experimentally validate. Comparative genomics and QTL analysis further shed light on the genetic foundation of the easily dehulled trait in a particular variety that was artificially selected by the Wa people, a minority group in Southwestern China known for cultivating Tartary buckwheat specifically for steaming as a staple food to prevent lysine deficiency. Conclusions This study provides both comprehensive insights into the origin and domestication of, and a foundation for molecular breeding for, Tartary buckwheat.

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Multiomics analysis reveals the molecular mechanisms underlying virulence in Rhizoctonia and jasmonic acid–mediated resistance in Tartary buckwheat (Fagopyrum tataricum)

Zhang

et al. 2023

The Plant Cell

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Rhizoctonia solani is a devastating soil-borne pathogen that seriously threatens the cultivation of economically important crops. Multiple strains with a very broad host range have been identified, but only one (AG1-IA, which causes rice sheath blight disease) has been examined in detail. Here, we analyzed AG4-HGI 3 originally isolated from Tartary buckwheat (Fagopyrum tataricum), but with a host range comparable to AG1-IA. Genome comparison reveals abundant pathogenicity genes in this strain. We used multi-omics approaches to improve the efficiency of screening for disease resistance genes. Transcriptomes of the plant-fungi interaction identified differentially expressed genes associated with virulence in Rhizoctonia and resistance in Tartary buckwheat. Integration with jasmonate-mediated transcriptome and metabolome changes revealed a negative regulator of jasmonate signaling, cytochrome P450 (FtCYP94C1), as increasing disease resistance probably via accumulation of resistance-related flavonoids. The integration of resistance data for 320 Tartary buckwheat accessions identified a gene homologous to aspartic proteinase (FtASP), with peak expression following R. solani inoculation. FtASP exhibits no proteinase activity but functions as an antibacterial peptide that slows fungal growth. This work reveals a potential mechanism behind pathogen virulence and host resistance, which should accelerate the molecular breeding of resistant varieties in economically essential crops.

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Facilitating viral vector movement enhances heterologous protein production in an established plant system

Cited by 3 publications

References 40 publications

Targeting of plasmodesmal proteins requires unconventional signals

Targeting of plasmodesmal proteins requires unconventional signals

Genomic insight into the origin, domestication, dispersal, diversification and human selection of Tartary buckwheat

Multiomics analysis reveals the molecular mechanisms underlying virulence in Rhizoctonia and jasmonic acid–mediated resistance in Tartary buckwheat (Fagopyrum tataricum)

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