Factor VIII: Von Willebrand Factor Patterns in the Plasma of Patients with Pre-Eclampsia

Calvin, Steve; Corrigan, James J.; Weinstein, Louis; Jeter, Monet

doi:10.1055/s-2007-999648

Cited by 17 publications

(6 citation statements)

References 3 publications

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“…The relative proportion of each fraction did not differ between patients with PIH and normal pregnancy controls. These findings are in agreement with a recent report (18).…”

Section: +6 38+5supporting

confidence: 94%

Von Willebrand Factor Multimer Patterns in Pregnancy-Induced Hypertension

et al. 1989

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SummaryMicroangiopathy and disseminated platelet aggregation have been reported in thrombotic thrombocytopenic purpura (TIP) and pregnancy-induced hypertension (PIH). Since unusually large von Willebrand factor (vWF) multimers have been implicated in the evolvement of TTP, we analyzed factor VIII/vWF parameters in patients with PIH. Mean vWF: Ag level was significantly higher in 27 patients with PIH as compared to 20 matched healthy pregnant women (358 ± 160 u/dl vs. 274 ± 125 u/dl, p < 0.05). Moreover, plasma vWF: Ag levels and the ratio of vWF: Ag to factor VIII were found to be linearly correlated to the severity of PIH. In contrast, no significant differences in mean levels of factor VIII and ristocetin cofactor were observed between these groups. Crossed immunoelectrophoresis of vWF revealed a higher incidence of a pre-peak and an increased migration index in the PIH group as compared to the control group (60% vs. 44% and 1.27 ± 0.26 vs. 1.19 ± 0.18, p < 0.01 respectively). Analysis of plasma vWF multimer patterns by 1.4% agarose electrophoresis in 0.1% SDS revealed excessive amounts of large, medium and small size multimers in the PIH patients. Conceivably, the quantitative changes in vWF multimers reflect endothelial injury and may play a role in the microangiopathy observed in PIH.

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“…The relative proportion of each fraction did not differ between patients with PIH and normal pregnancy controls. These findings are in agreement with a recent report (18).…”

Section: +6 38+5supporting

confidence: 94%

Von Willebrand Factor Multimer Patterns in Pregnancy-Induced Hypertension

et al. 1989

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“…None of the control patients had bleeding complications during delivery or thrombocytopenia. These findings are in agreement with data from Calvin et al (3) who studied the antepartum vWF multimeric distribution in pre-eclamptic patients and normal controls.…”

Section: Commentssupporting

confidence: 93%

“…Under certain stressful conditions vWF is released in larger amounts, particularly multimers of very high molecular weight. Alterations of the multimeric distribution of vWF are presumed to be related to the pathogenesis and thrombocytopenia of thrombotic thrombocytopenic purpura (2) and in severe pre-eclampsia (3). Both conditions share some clinical and laboratory findings such as thrombocytopenia.…”

Section: Introductionmentioning

confidence: 99%

The Role of von Willebrand Factor in Pre-Eclampsia

et al. 1991

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SummaryThe von Willebrand factor (vWF) has gained considerable interest in recent years as a marker of endothelial cell activation or insult and by virtue of its interactions with platelets and vessel walls. Altered patterns of vWF multimers were found to occur frequently in patients with thrombotic thrombocytopenic purpura in the acute and chronic stages. This disorder shares some clinical and laboratory findings with pre-eclampsia, including thrombocytopenia. Recent studies have also suggested that abnormalities of endothelial cell metabolism play a central role in the pathophysiology of pre-eclampsia. In order to determine if vWF could be instrumental in the disease process and the thrombocytopenia of pre-eclampsia we analyzed the ante- and postpartum structural and functional distribution of vWF. This data was correlated with hematological parameters such as platelet counts and the clinical severity of the disease. We found no consistent changes of vWF in association with thrombocytopenia or clinical severity. However, functional vWF was lower in postpartum samples of severely affected pre-eclamptics as compared to normal controls. This finding may reflect endothelial cell exhaustion after stimulation or cellular injury. Elevated titers of fibrin split products and thrombocytopenia were evident in severe pre-eclampsia, as seen in DIC, despite factor VIII coagulant levels within the normal range. Our data is consistent with the hypothesis of endothelial cell dysfunction in pre-eclampsia. However, the mechanism of thrombocytopenia in this disorder does not appear to be related to alterations in the structure or biological function of vWF.

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“…(B) Fibrinogen concentrations in women with preeclampsia (PE) (n = 44), in women with non-preeclampsia complications of pregnancy (Non-PE) (n = 44) and in uncomplicated pregnancy (Normal) (n = 44), analyzed by Student's t-test. group (data not shown), but this did not reach a significant value (p = 0.06), similar to the findings of Calvin and coworkers(17).…”

supporting

confidence: 89%

Fibrinogen Concentration and Factor VIII Activity in Women with Preeclampsia

Williams

Griffiths

Carbone

et al. 2007

Hypertension in Pregnancy

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This study has shown a novel increase in both fibrinogen concentration and factor VIII activity in pregnant women with preeclampsia compared with values obtained from women with normal or non-preeclampsia complicated pregnancies, with women with preeclampsia twice as likely to have a raised fibrinogen concentration and increased factor VIII activity. These changes may contribute to the hypercoagulability seen in preeclampsia.

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Factor VIII: Von Willebrand Factor Patterns in the Plasma of Patients with Pre-Eclampsia

Cited by 17 publications

References 3 publications

Von Willebrand Factor Multimer Patterns in Pregnancy-Induced Hypertension

Von Willebrand Factor Multimer Patterns in Pregnancy-Induced Hypertension

The Role of von Willebrand Factor in Pre-Eclampsia

Fibrinogen Concentration and Factor VIII Activity in Women with Preeclampsia

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