Factorial design as tool in chitosan nanoparticles development by ionic gelation technique

Neves, Andréia Lange de Pinho; Milioli, Camila Cardoso; Müller, Leidiani; Riella, Humberto Gracher; Kuhnen, Nivaldo Cabral; Stulzer, Hellen Karine

doi:10.1016/j.colsurfa.2013.12.058

Cited by 113 publications

(66 citation statements)

References 44 publications

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“…However, more pronounced and continuous size increase was noticed when chitosan to TPP ratio increased from formulation CH5 to CH8 (p < 0.05). Those results are confirmed by other data reported in literature [20]. Obtained zeta potentials were all positive and they ranged from þ21 to þ27 mV (See Fig.…”

Section: Resultssupporting

confidence: 95%

Enhancement of alendronate encapsulation in chitosan nanoparticles

Miladi

Sfar

Fessi

et al. 2015

Journal of Drug Delivery Science and Technology

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Section: Resultssupporting

confidence: 95%

Enhancement of alendronate encapsulation in chitosan nanoparticles

Miladi

Sfar

Fessi

et al. 2015

Journal of Drug Delivery Science and Technology

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“…Ethanol and Tween-80 (1:1 v/v) were used as the organic solvent and detergent, respectively, for MIF because of their low toxic potential and emulsifying activity for dissolution of the hydrophobic components. In the preparation process, it was reported that several factors such as Cs concentration, TPP concentration, Cs/TPP mass ratio could have an influence on particle size, encapsulation efficiency (EE), and drug loading capacity (DL) of the nanoparticles [23,26]. In order to select optimum conditions for preparation of MCNs, the effects of Cs concentration, TPP concentration, and Cs/MIF mass ratio on the EE and DL of nanoparticles were investigated [25].…”

Section: Resultsmentioning

confidence: 99%

“…The results showed that the horizontal distance of MCNs increased from about 150 nm to 200 nm and the vertical distance of MCNs increased from about 20 nm to 30 nm after MIF entrapment. It was reported that the concentration of TPP and the concentration of Cs have effects on the size of chitosan nanoparticles [26,29]. However, considering that the gastrointestinal absorption and bioavailability of nanoparticles designed for oral administration was closely related to the properties of the composition and the ingredient for specific targeting [30], the influencing factors of the size of MCNs were not studied in this work.…”

Section: Resultsmentioning

confidence: 99%

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

Zhang¹,

Wu²,

Li³

et al. 2016

Beilstein J. Nanotechnol.

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In addition to its well-known abortifacient effect, mifepristone (MIF) has been used as an anticancer drug for various cancers in many studies with an in-depth understanding of the mechanism of action. However, application of MIF is limited by its poor water solubility and low oral bioavailability. In this work, we developed a drug delivery system based on chitosan nanoparticles (CNs) to improve its bioavailability and anticancer activity. The MIF-loaded chitosan nanoparticles (MCNs) were prepared by convenient ionic gelation techniques between chitosan (Cs) and tripolyphosphate (TPP). The preparation conditions, including Cs concentration, TPP concentration, Cs/MIF mass ratio, and pH value of the TPP solution, were optimized to gain better encapsulation efficiency (EE) and drug loading capacity (DL). MCNs prepared with the optimum conditions resulted in spherical particles with an average size of 200 nm. FTIR and XRD spectra verified that MIF was successfully encapsulated in CNs. The EE and DL of MCNs determined by HPLC were 86.6% and 43.3%, respectively. The in vitro release kinetics demonstrated that MIF was released from CNs in a sustained-release manner. Compared with free MIF, MCNs demonstrated increased anticancer activity in several cancer cell lines. Pharmacokinetic studies in male rats that were orally administered MCNs showed a 3.2-fold increase in the area under the curve from 0 to 24 h compared with free MIF. These results demonstrated that MCNs could be developed as a potential delivery system for MIF to improve its anticancer activity and bioavailability.

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“…Reduction of the particle size is a strategy to optimize the activity of chitosan due to the increased power of the concentrated positive charge and enlargement of the contact surface area. A bottom-up process such as size modification can be carried out using precipitation [11], ionic gelation [12,13] or the polyelectrolyte complex method [14]. All three methods are are widely used because they are simple and inexpensive.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Formulation Methods to Produce Nano-Chitosan as Inhibitor Agent for Bacterial Growth

Nugraheni

Soeriyadi

Ustadi

et al. 2019

J. Eng. Technol. Sci.

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Chitosan is known as an antibacterial agent. The effective surface area ratio of chitosan can be increased by modification into nanoparticles. Nanochitosan can be prepared with several simple methods, i.e. precipitation, ionic gelation, or the polyelectrolyte complex method. This study compared these three methods in terms of the targeted product characteristics, i.e. stability of the average nanoparticle size as well as the colloidal dispersion, and the antibacterial characteristics. All three methods resulted in nanoparticle formation, but in the precipitation method significant zeta potential reduction was observed due to the presence of negative ions from the alkali that neutralized the chitosan amine group. The ionic gelation method yielded higher zeta potential and higher inhibition of bacterial growth than those yielded by the polyelectrolyte complex method. Ionic gelation and the polyelectrolyte complex method resulted in much better colloidal dispersion stability than the precipitation method, where a significant particle size increase was observed after one week of storage. This result indicates that both ionic gelation and the polyelectrolyte complex method can be used for forming nano-chitosan for the purpose of food preservation. However, for fishery products it is advisable to use the polyelectrolyte complex method because the TPP usually used in ionic gelation is not allowed to be applied to fish.

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Factorial design as tool in chitosan nanoparticles development by ionic gelation technique

Cited by 113 publications

References 44 publications

Enhancement of alendronate encapsulation in chitosan nanoparticles

Enhancement of alendronate encapsulation in chitosan nanoparticles

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

Comparison of Formulation Methods to Produce Nano-Chitosan as Inhibitor Agent for Bacterial Growth

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