1992
DOI: 10.1002/bit.260391010
|View full text |Cite
|
Sign up to set email alerts
|

Factors affecting 2‐hydroxypropiophenone formation by benzoylformate decarboxylase from Pseudomonas putida

Abstract: Benzoylformate (100 mM) was quantitatively converted to the acyloin compound, 2-hydroxypropiophenone (61.76 mM) and benzaldehyde (38.2 mM) by an enzyme extract from Pseudomonas putida ATCC 12633 in the presence of 1.6M acetaldehyde. Biotransformations were carried out at pH 6.0 and 30 degrees C with an incubation time of 60 min. Activity of the acyloin forming enzyme, benzoylformate decarboxylase, was 1.23 units/mL in the biotransformation mixture. Acyloin formation increased dramatically with pH in the range … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4

Citation Types

0
22
0

Year Published

2000
2000
2022
2022

Publication Types

Select...
5
5

Relationship

0
10

Authors

Journals

citations
Cited by 48 publications
(22 citation statements)
references
References 19 publications
0
22
0
Order By: Relevance
“…The most industrially important lipases do not require cofactors for activity. BFD catalyses the enantioselective carboligase reaction leading to a broad range of carbonyl 2-hydroxy ketones, useful as synthons for organic chemistry [25][26][27][28]. These are important intermediates in the production of antifungals [29] as well as of pharmaceuticals like Wellbutrin Ò (treatment of depression) and Zyban Ò (smoking cessation) (both from Glaxo Wellcome) [30].…”
Section: Introductionmentioning
confidence: 99%
“…The most industrially important lipases do not require cofactors for activity. BFD catalyses the enantioselective carboligase reaction leading to a broad range of carbonyl 2-hydroxy ketones, useful as synthons for organic chemistry [25][26][27][28]. These are important intermediates in the production of antifungals [29] as well as of pharmaceuticals like Wellbutrin Ò (treatment of depression) and Zyban Ò (smoking cessation) (both from Glaxo Wellcome) [30].…”
Section: Introductionmentioning
confidence: 99%
“…BFD was first reported by Wilcocks et al (1992). The enzyme is involved in the nonoxidative decarboxylation of benzoylformate (Iding et al, 2000), and is able to catalyze the enantioselective synthesis of (S)-2-hydroxypropanone derivatives (Demir et al, 1999).…”
Section: Introductionmentioning
confidence: 99%
“…This is in agreement with previous data, and demonstrates a high stability of BFD in the presence of acetaldehyde. [27,56] A potential reason for the deactivation of BAL in the presence of propanal can be the formation of Schiff-bases. Propanal can react with the protein, in particular with primary amino groups, to form a complex that can deactivate the enzyme.…”
mentioning
confidence: 99%