Factors Affecting Developmental Processes in Alfalfa Cell Cultures

Stuart, David A.; Nelsen, Janet; Strickland, Steven G.; Nichol, James W.

doi:10.1007/978-1-4899-0378-5_5

Cited by 47 publications

(20 citation statements)

References 21 publications

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“…Residual auxin has been reported to inhibit embryogenesis in celery, but somatic embryogenesis could be restored by addition of cytokinin (Stuart et al, 1985). A similar response to cytokinin with P. x hortorum might exist.…”

Section: Discussionmentioning

confidence: 84%

“…Cytokinin effects on somatic embryogenesis have been studied less intensively, however, they have been recognized for their modulating effect on somatic embryogenesis (George and Sherrington, 1984;Stuart et al, 1985). Residual auxin has been reported to inhibit embryogenesis in celery, but somatic embryogenesis could be restored by addition of cytokinin (Stuart et al, 1985).…”

Section: Discussionmentioning

confidence: 97%

“…The role of auxins for induction of somatic embryogenesis has been emphasized (Sharp et al, 1980;Maheshwaran and Williams, 1986) and cytokinins have also been reported to improve somatic embryo yield in some systems, where endogenous auxin may inhibit embryo development (Stuart et al, 1985). Geranium is a popular ornamental species and the development of tissue culture methods to propagate it appear to be economically justified since the hybrid geranium seeds are expensive and most of the tetraploid geraniums are propagated by cuttings.…”

Section: Introductionmentioning

confidence: 99%

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Improvement of somatic embryogenesis in zonal geranium

Wilson

Sullivan

Marsolais

et al. 1996

Plant Cell Tiss Organ Cult

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A number of media constituents including sucrose, ammonium nitrate and plant growth regulators were evaluated in an attempt to improve somatic embryo production in zonal geranium (Pelargonium x hortorum) cv. Scarlet Orbit Improved. Somatic embryo production was characterized by the quantity and type of somatic embryo induced by the treatments. Sucrose at 4% supported the highest number of total somatic embryos while improving the proportion of the morphologically normal cotyledon-stage somatic embryos. Addition of ammonium nitrate also improved embryo production. With 1.89 mM ammonium nitrate, normal cotyledon-stage embryo development was increased by 53%; the proportion of normal cotyledon-stage embryos decreased and abnormal embryos with leaves or serrated margins in cotyledons (fringed-shoot type) increased with higher ammonium nitrate concentrations. The effect of plant growth regulators on somatic embryogenesis indicated that exogenous supply of indole-3-acetic acid (IAA) at a range of 0.25 to 4/zM failed to promote somatic embryogenesis. In contrast, benzyladenine (BA) up to 2.0 #M increased the total embryo number and the proportion of desirable cotyledon-stage embryos. There was no interaction between IAA and BA. Our research has demonstrated that improvement in both quantity and quality of somatic embryos can be achieved in zonal geranium.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Improvement of somatic embryogenesis in zonal geranium

Wilson

Sullivan

Marsolais

et al. 1996

Plant Cell Tiss Organ Cult

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“…This parameter is, however, of importance to the application of embryogenesis systems, as the efficiency of conversion of embryos to plantlets often varies considerably with embryo normality [4,21]. The major factor affecting soybean somatic embryo morphology is auxin type, although other physical and chemical factors are also involved [ 13].…”

Section: Discussionmentioning

confidence: 99%

Soybean somatic embryogenesis: Effects of hormones and culture manipulations

Lazzeri

Hildebrand

Collins

1987

Plant Cell Tiss Organ Cult

132

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Somatic embryos were induced in cultures of immature soybean (Glycine max (L.) Merr) embryos, or isolated cotyledons on MS modified medium supplemented with NAA and 2,4-D, BAP and ABA. When NAA and 2,4-D were compared at similar concentrations (25 and 23 #M), 2,4-D produced larger number of somatic embryos, however, embryogenesis efficiency was improved in media containing NAA by using higher levels (100-150 #M) of the auxin. Somatic embryo morphology varied with auxin type: NAA-induced embryos more closely resembled zygotic embryos than did 2,4-D-induced embryos. Additions of BAP or ABA to auxin-containing media had either no effect or reduced embryo production, although ABA altered the morphology of 2,4-D-induced embryos. In media containing both NAA and 2,4-D, the latter was dominant in terms of embryo morphology. The effects of subculture frequency and of transfers between 2,4-D and NAA media were investigated: Subculture frequency influenced mainly the frequency of normal embryos, while preculture on 2,4-D increased subsequent embryogenesis efficiency on NAA medium but reduced the frequency of normal embryos.

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“…(3) Somatic embryogenesis is the principal pathway of regeneration in lucerne (Stuart et al, 1985), but in protoplast culture it occurs more often with an intervening callus stage. The presence of 2,4-0 in culture medium is usually required for induction of somatic embryogenesis (Saunders and Bingham, 1972;Davies, 1988).…”

Section: Protoplast Culture and Regeneration: A Case Study Of Medicagmentioning

confidence: 99%