Factors affecting hepatocyte isolation, engraftment, and replication in an in vivo model

Kawahara, Toshiyasu; Toso, Christian; Douglas, Donna N.; Nourbakhsh, Mahra; Lewis, Jamie; Tyrrell, D. Lorne; Lund, Garry; Churchill, Thomas A.; Kneteman, Norman M.

doi:10.1002/lt.22177

Cited by 19 publications

(1 citation statement)

References 25 publications

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“…Human explanted liver tissue was used to isolate hepatocytes [15], hepatic stellate cells (HSC) [16], activated liver myofibroblasts (aLMF) [16], sinusoidal endothelial cells (HSEC) [16] and biliary epithelial cells (BEC) [17] according to our standard protocols.…”

Section: Human Primary Liver Cell Culturesmentioning

confidence: 99%

Ketohexokinase inhibition improves NASH by reducing fructose-induced steatosis and fibrogenesis

et al. 2021

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Section: Human Primary Liver Cell Culturesmentioning

confidence: 99%

Ketohexokinase inhibition improves NASH by reducing fructose-induced steatosis and fibrogenesis

et al. 2021

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Clinical Hepatocyte Transplantation: What Is Next?

et al. 2017

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Purpose of review Significant recent scientific developments have occurred in the field of liver repopulation and regeneration. While techniques to facilitate liver repopulation with donor hepatocytes and different cell sources have been studied extensively in the laboratory, in recent years clinical hepatocyte transplantation (HT) and liver repopulation trials have demonstrated new disease indications and also immunological challenges that will require the incorporation of a fresh look and new experimental approaches. Recent findings Growth advantage and regenerative stimulus are necessary to allow donor hepatocytes to proliferate. Current research efforts focus on mechanisms of donor hepatocyte expansion in response to liver injury/preconditioning. Moreover, latest clinical evidence shows that important obstacles to HT include optimizing engraftment and limited duration of effectiveness, with hepatocytes being lost to immunological rejection. We will discuss alternatives for cellular rejection monitoring, as well as new modalities to follow cellular graft function and near-to-clinical cell sources. Summary HT partially corrects genetic disorders for a limited period of time and has been associated with reversal of ALF. The main identified obstacles that remain to make HT a curative approach include improving engraftment rates, and methods for monitoring cellular graft function and rejection. This review aims to discuss current state-of-the-art in clinical HT and provide insights into innovative approaches taken to overcome these obstacles.

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Experimental Cell Therapy for Liver Dysfunction

Wang¹,

Wertheim

2016

Translating Regenerative Medicine to the Clinic

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Factors affecting hepatocyte isolation, engraftment, and replication in an in vivo model

Cited by 19 publications

References 25 publications

Ketohexokinase inhibition improves NASH by reducing fructose-induced steatosis and fibrogenesis

Ketohexokinase inhibition improves NASH by reducing fructose-induced steatosis and fibrogenesis

Clinical Hepatocyte Transplantation: What Is Next?

Experimental Cell Therapy for Liver Dysfunction

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