Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: Effect of co-substrates, operation mode and oxygen

Pereira, Ana Sofia; Miranda, Sílvia M.; Lopes, Marlene; Belo, Isabel

doi:10.1016/j.jbiotec.2021.02.014

Cited by 34 publications

(34 citation statements)

References 41 publications

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“…However, crude glycerol is not pure, and the purification is not a cost‐effective process, which limits its traditional applications 28 . Even so, crude glycerol can be directly used with high efficiency by Y. lipolytica 26,29 …”

Section: Introductionmentioning

confidence: 99%

“…28 Even so, crude glycerol can be directly used with high efficiency by Y. lipolytica. 26,29 Furthermore, the choice of mode of operation is especially important in the case of 2-PE production, considering its toxicity to producing cells. In this way, it is possible to select an operation mode capable of removing or diluting the toxic product from the medium, reducing its inhibitory effect, and maximizing productivity.…”

Section: Introductionmentioning

confidence: 99%

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Valorization of crude glycerol as carbon source for the bioconversion of L‐phenylamine to 2‐phenylethanol by Yarrowia species

Braga

Freitas

Cordeiro

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND 2‐Phenylethanol (2‐PE) is an aromatic alcohol with a delicate fragrance of rose petals that is widely used in perfumes, cosmetics, foods, beverages, and pharmaceutical industries. The yeast Yarrowia can produce 2‐PE through L‐Phenylalanine (L‐Phe) biotransformation. However, it has not been properly explored in this field, despite its interesting characteristics for this process, such as the Crabtree negative trait. RESULTS This study explored the potential of different Yarrowia species (Y. lipolytica W29, CH 1/5, and CH 3/4, as well as Y. divulgata M 445/4) for 2‐PE production. It was observed that all strains were inhibited by 2‐PE concentrations above 2 g L−1, but only Y. lipolytica W29 was inhibited by 15 g L−1 L‐Phe. It was also confirmed that all strains were able to produce 2‐PE, with concentrations ranging from 1 to 2.2 g L−1. Afterwards, crude glycerol was used as an alternative carbon source for 2‐PE production. All strains grew and produced 2‐PE on this carbon source, but CH 1/5 strain was selected for further studies because it allowed the highest 2‐PE throughput. The production of 2‐PE with CH 1/5 was further studied in bioreactor batch cultures, where a low accumulation of 2‐PE was observed. However, a step‐wise fed‐batch culture allowed an increase in the production of 2‐PE to 3.2 g L−1. CONCLUSION To the best of the authors' knowledge, this value corresponds to the highest titer of 2‐PE obtained to date with Y. lipolytica. In this study, Y. lipolytica CH 1/5 is, for the first time, described as a good natural producer for 2‐PE. © 2021 Society of Chemical Industry (SCI).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Valorization of crude glycerol as carbon source for the bioconversion of L‐phenylamine to 2‐phenylethanol by Yarrowia species

Braga

Freitas

Cordeiro

et al. 2021

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

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“…Microbial lipid is an important oil resource, which has been widely used for the production of biofuels, pharmaceutics, cosmetics, food additives, and renewable polymers . Generally, microbial cells possess two main synthetic pathways for lipid synthesis, namely, de novo and ex novo synthetic ones.…”

Section: Introductionmentioning

confidence: 99%

“…Generally, microbial cells possess two main synthetic pathways for lipid synthesis, namely, de novo and ex novo synthetic ones. In nature, a variety of microorganisms including fungi, bacteria, and algae have been reported to have the capabilities to de novo synthesize microbial lipids, such as Yarrowia lipolytica, , Rhodotorula glutinis, Aspergillus ochraceus, Ashbya gossypii, and Gracilaria corticata. …”

Section: Introductionmentioning

confidence: 99%

“…1 Generally, microbial cells possess two main synthetic pathways for lipid synthesis, namely, de novo and ex novo synthetic ones. In nature, a variety of microorganisms including fungi, bacteria, and algae have been reported to have the capabilities to de novo synthesize microbial lipids, such as Yarrowia lipolytica, 1,2 Rhodotorula glutinis, 3 Aspergillus ochraceus, 4 Ashbya gossypii, 5 and Gracilaria corticata. 6 Currently, oleaginous yeasts are the most widely used microorganisms for microbial lipid production, especially Y. lipolytica, which is "generally recognized as safe" (GRAS) by the US Food and Drug Administration.…”

Section: Introductionmentioning

confidence: 99%

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Increased Lipid Production in Yarrowia lipolytica from Acetate through Metabolic Engineering and Cosubstrate Fermentation

et al. 2021

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Bioconversion of acetate, a byproduct generated in industrial processes, into microbial lipids using oleaginous yeasts offers a promising alternative for the economic utilization of acetate-containing waste streams. However, high acetate concentrations will inhibit microbial growth and metabolism. In this study, the acetate utilization capability of Yarrowia lipolytica PO1f was successively improved by overexpressing the key enzyme of acetyl-CoA synthetase (ACS), which resulted in an accumulation of 9.2% microbial lipids from acetate in shake flask fermentation. By further overexpressing the second key enzymes of acetyl-CoA carboxylase (ACC1) and fatty acid synthase (FAS) in Y. lipolytica, the lipid content was increased to 25.7% from acetate. Finally, the maximum OD600 of 29.2 and a lipid content of 41.7% were obtained with the engineered strain by the adoption of cosubstrate (glycerol and acetate) fed-batch fermentation, which corresponded to an increase of 68 and 95%, respectively. These results presented a promising strategy for economic and efficient microbial lipid production from the waste acetate.

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Bio-oil production for biodiesel industry by Yarrowia lipolytica from volatile fatty acids in two-stage batch culture

Pereira

Lopes

Miranda

et al. 2022

Appl Microbiol Biotechnol

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Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: Effect of co-substrates, operation mode and oxygen

Cited by 34 publications

References 41 publications

Valorization of crude glycerol as carbon source for the bioconversion of L‐phenylamine to 2‐phenylethanol by Yarrowia species

Valorization of crude glycerol as carbon source for the bioconversion of L‐phenylamine to 2‐phenylethanol by Yarrowia species

Increased Lipid Production in Yarrowia lipolytica from Acetate through Metabolic Engineering and Cosubstrate Fermentation

Bio-oil production for biodiesel industry by Yarrowia lipolytica from volatile fatty acids in two-stage batch culture

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