Factors Affecting Plant Regeneration from Leaf Tissues of Buddleia Species

Abstract

The effects of genotype, basal medium, plant growth regulator (PGR), dark treatment, and antibiotics on shoot regeneration of two Buddleia cultivars, B. davidii 'Potters Purple' and Buddleia 'Lochinch', were investigated. In vitro shoots were regenerated from leaf tissues in either Murashige and Skoog (MS) or woody plant medium (WPM) media supplemented with benzyladenine (BA). In general, more shoots were regenerated in WPM medium than in MS medium. Dark treatment for 3 to 5 weeks dramatically increased shoot regeneration. Addition of indole-3-butyric acid (IBA) or naphthalene acetic acid (NAA) significantly enhanced the regeneration rate and shoots of each explant. The maximum regeneration rate (100%) of B. davidii 'Potters Purple' was achieved when cultured in WPM containing 5 mM BA plus 5 mM IBA. The maximum regeneration rate (98.4%) of Buddleia 'Lochinch' was found in WPM supplemented with 20 mM BA plus 4 mM IBA. Carbenicillin at 250 to 500 mgÁL -1 and cefotaxime at 125 to 250 mgÁL -1 , individually or combined, promoted shoot regeneration. Interactions between genotype and medium or PGRs were found. In vitro shoots were easily rooted in halfstrength MS medium with or without NAA. Rooted plants were transferred to potting mix and grown in the greenhouse. This research will facilitate genetic improvement and fast propagation of Buddleia species using biotechnology.

Effects of β-lactam antibiotics, auxins, and cytokinins on shoot regeneration from callus cultures of two hybrid aspens, Populus tremuloides × P. tremula and P. x canescens × P. gradidentata

“…In more recent research caulogenesis was facilitated by CARB when the shoots were regenerated indirectly from callus (Suzuki et al 1998;Bhau and Wakhlu 2001;Tang et al 2004). Positive results have also been reported for direct regeneration protocols when an initial dark culture period, that would be expected to stimulate callusing prior to the onset of shoot differentiation, was included (Billings et al 1997;Hammerschlag et al 1997;Dai and Castillo 2007).…”

Notice of Retraction: Kinetics and Selections of Process Protein Synthesis Culture in Five Alfalfa

“…The experimental data has been fitted into Monod equations. [4] , Organic ingredients are take in B5 [5] .Culture media used in this study were supplemented with 3% sucrose and 0.7% agar [6] .…”

Plant regeneration from in vitro leaf tissues of Viburnum dentatum L

“…Limited and controversial information is available regarding the effect of dark treatment on plant regeneration. It has been reported that dark treatment significantly increased plant regeneration (Gu and Zhang 2005;Ahn et al 2007;Dai and Castillo 2007); while other research has shown no positive effect of dark treatment on plant regeneration (Sarma and Rogers 1998). Little is known about why dark treatment influences in vitro regeneration.…”