Factors Affecting Polyhydroxybutyrate Biosynthesis In the Marine Photosynthetic Bacterium Rhodopseudomonas sp. Strain W-1 S

ry, Wasimul Q. Chowdhu; Idehara, Kenji; Maeda, Isamu; Umeda, Fusako; Yagi, Kiyohito; Miura, Yoshiharu; Mizoguchi, Tadashi

doi:10.1007/978-1-4612-0223-3_31

Cited by 7 publications

(15 citation statements)

References 8 publications

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“…PHA accumulation is known to be enhanced when excess carbon is present and other nutrient(s), such as nitrogen, phosphorus or sulfur, are limited [ 18 ]. In the case of photosynthetic bacteria, PHA production can be induced under nitrogen- or vitamin-limited conditions [ 10 ], [ 13 ]. In addition, acetic acid was shown to be an efficient carbon source for PHA production in photosynthetic bacteria [ 13 , 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…It was reported that Rhodobacter sphaeroides and Rhodopseudomonas palustris accumulated PHA levels of 60–70 wt% [ 11 ] and 4 wt% [ 12 ], respectively. In contrast, studies of PHA production in marine photosynthetic bacteria are limited to only a few strains [ 13 , 14 ]. The marine purple non-sulfur bacterium Rhodovulum sulfidophilum reportedly possesses the ability to synthesize poly[( R )-3-hydroxybutyrate] (PHB) up to 50 wt% of its dry weight [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, studies of PHA production in marine photosynthetic bacteria are limited to only a few strains [ 13 , 14 ]. The marine purple non-sulfur bacterium Rhodovulum sulfidophilum reportedly possesses the ability to synthesize poly[( R )-3-hydroxybutyrate] (PHB) up to 50 wt% of its dry weight [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria

et al. 2016

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Polyhydroxyalkanoate (PHA) is a biopolyester/bioplastic that is produced by a variety of microorganisms to store carbon and increase reducing redox potential. Photosynthetic bacteria convert carbon dioxide into organic compounds using light energy and are known to accumulate PHA. We analyzed PHAs synthesized by 3 purple sulfur bacteria and 9 purple non-sulfur bacteria strains. These 12 purple bacteria were cultured in nitrogen-limited medium containing acetate and/or sodium bicarbonate as carbon sources. PHA production in the purple sulfur bacteria was induced by nitrogen-limited conditions. Purple non-sulfur bacteria accumulated PHA even under normal growth conditions, and PHA production in 3 strains was enhanced by nitrogen-limited conditions. Gel permeation chromatography analysis revealed that 5 photosynthetic purple bacteria synthesized high-molecular-weight PHAs, which are useful for industrial applications. Quantitative reverse transcription polymerase chain reaction analysis revealed that mRNA levels of phaC and PhaZ genes were low under nitrogen-limited conditions, resulting in production of high-molecular-weight PHAs. We conclude that all 12 tested strains are able to synthesize PHA to some degree, and we identify 5 photosynthetic purple bacteria that accumulate high-molecular-weight PHA molecules. Furthermore, the photosynthetic purple bacteria synthesized PHA when they were cultured in seawater supplemented with acetate. The photosynthetic purple bacteria strains characterized in this study should be useful as host microorganisms for large-scale PHA production utilizing abundant marine resources and carbon dioxide.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria

et al. 2016

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“…The net effect of PHB degradation, at which PHB biosynthesis did not occur, on hydrogen production could be assessed using succinate (Fig. 5) because only a small amount of PHB was converted from succinate (Chowdhury et al, 1996;Maeda et al, 1997). The conversion of organic compounds to hydrogen in phototrophic bacteria is known to be a completely light-driven process and its efficiency depends on the kind of organic .…”

Section: Contribution Of Phb Degradation To Growth and Hydrogen Produmentioning

confidence: 99%

“…A marine phototrophic, purple nonsulfur bacterium, Rhodovulum sulfidophilum W-1S, was isolated as a catalyst for hydrogen production using a seawater-based medium (Maeda et al, 2000;Miura et al, 1992). Cells of W-1S were shown to possess the ability to accumulate PHB at over 50% of cell dry weight when acetate was used as a substrate (Chowdhury et al, 1996). Hydrogen production from the accumulated PHB as an endogenous substrate was demonstrated in a cell suspension of W-1S in the absence of an external substrate (Maeda et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Maximization of hydrogen production ability in high‐density suspension of Rhodovulum sulfidophilum cells using intracellular poly(3‐hydroxybutyrate) as sole substrate

Maeda

Miyasaka

Umeda

et al. 2002

Biotech & Bioengineering

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Growth of and hydrogen production by wild-type (WT) Rhodovulum sulfidophilum were compared with those by one of its mutants lacking the poly(3-hydroxybutyrate) (PHB) biosynthesis ability (PNM2). During phototrophic growth under aerobic conditions with fixed illumination, changes in the extinction coefficient and PHB content of WT and PNM2 cells revealed interference of light penetration by PHB. WT cells synthesized PHB at an early stage of the cultivation. PHB degradation after exhaustion of acetate during the cultivation of WT resulted in a decrease of the extinction coefficient. The hydrogen production rate under anaerobic conditions with fixed illumination was examined in WT and PNM2 cell suspensions at different densities. The hydrogen production rate was determined not by the light penetration but by the kinds of hydrogen donors and the density of suspension. The highest value of the rate of hydrogen production from PHB, 33.0 ml/l/h, was improved compared with 26.6 ml/l/h, which was the highest value in hydrogen production from succinate. Under the same illumination, conversion to hydrogen from PHB is more efficient than that from succinate, which is one of the best substrates for hydrogen production. These results suggest that the hydrogen production rate can be maximized in the hydrogen production system based on PHB degradation, which is achieved in high-density suspension under external-substrate-depleted conditions after aerobic cultivation in the presence of an excess amount of acetate.

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Effects of pH, temperature and salinity on P3HB synthesis culturing the marine Rhodovulum sulfidophilum DSM-1374

Carlozzi

Lorenzo

Ghanotakis

et al. 2020

Appl Microbiol Biotechnol

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Factors Affecting Polyhydroxybutyrate Biosynthesis In the Marine Photosynthetic Bacterium Rhodopseudomonas sp. Strain W-1 S

Cited by 7 publications

References 8 publications

Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria

Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria

Maximization of hydrogen production ability in high‐density suspension of Rhodovulum sulfidophilum cells using intracellular poly(3‐hydroxybutyrate) as sole substrate

Effects of pH, temperature and salinity on P3HB synthesis culturing the marine Rhodovulum sulfidophilum DSM-1374

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