The effect of different nitrogen (N) concentrations on growth changes, leaf N concentration and accumulation patterns, N nutrition index (NNI), fresh weight yield, and N use efficiency (NUE) was determined for lettuce grown over three consecutive seasons (fall, winter, and spring) in a recirculating hydroponic system, under unheated and naturally lit hoop house in Uvalde, TX. The lettuce cultivars Buttercrunch, Dragoon, and Sparx were grown at six N concentrations, initially 100, 150, 200, 250, 300, and 400 mg · L L1 using a nutrient film technique (NFT). Leaf number, accumulated dry weight (DW) and N, and leaf area index (LAI) followed a logistic trend over time, characterized by a slow increase during early growth followed by a linear increase to a maximum. By contrast, plant total N concentrations were the highest at early stage and decreased slightly over time. Effect of season and cultivar on these growth traits was more pronounced than that of the N concentrations. Averaged across cultivar and N concentrations, DW in spring was 73% and 34% greater than that in fall and winter, respectively. At each sampling date, there were linear, quadratic, or cubic effects of N concentrations on each of these variables. The cultivar Sparx was the most productive, with 63% and 32% higher fresh weight yield in fall, 145% and 114% in spring, than 'Buttercrunch' and 'Dragoon', respectively. Increasing nutrient solution N concentrations from 100 to 400 mg · L L1 increased the yield from 5.9 to 6.7 kg · m L2 in fall, 8.1 to 10.7 kg · m L2 in winter, and 10.3 to 12.6 kg · m L2 in spring. The NUE was the highest at the lowest N concentration (100 mg · L L1 ) and decreased with increasing N concentrations. The NNI during mid-to late-growth stages was near or greater than one, even at the lowest N. These results demonstrated that N concentrations of 100-150 mg · L L1 maximized the growth and yield of hydroponically grown lettuce.