Factors affecting the performance of different long terminal repeats in the retroviral vector

Kim, Sujeong; Lee, Karim; Kim, Mi-Dong; Kang, S.W.; Joo, Chang Wan; Kim, Jong-Mook; Kim, Seon Hee; Yu, Seung Shin; Kim, Sun‐Young

doi:10.1016/j.bbrc.2006.03.035

Cited by 10 publications

(10 citation statements)

References 22 publications

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“…We employed a strong MSCV promoter in our vectors, and the previous group used the spleen focus-forming virus promoter (3). However, expression from these two promoters has been shown to be comparable on May 9, 2018 by guest http://jvi.asm.org/ (28). Instead, the most likely explanation is that the prior study used unconcentrated vector stocks with undetermined titers, while we concentrated our NIFV vectors more than 100-fold and infected with known MOIs of genome-containing particles.…”

Section: Discussionmentioning

confidence: 94%

Nonintegrating Foamy Virus Vectors

Deyle¹,

Li²,

Olson³

et al. 2010

J Virol

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Section: Discussionmentioning

confidence: 94%

Nonintegrating Foamy Virus Vectors

Deyle¹,

Li²,

Olson³

et al. 2010

J Virol

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“…Four of them have the same backbone called MT, except for the 3¢ LTR region. 5 MT is a minimum size Moloney murine leukemia virus (MoMLV)-based vector containing no viral coding sequences. 6 The MoMLV 3¢LTR of MT was replaced with LTRs from MSCV, myeloproliferative sarcoma virus (MPSV) and spleen focus-forming virus (SFFV) to produce three different retroviral vectors called MS, MP and MF, respectively ( Figure 1).…”

Section: Retroviral Vectors Used In the Studymentioning

confidence: 99%

“…2,5 The eGFP sequence was amplified using primers eGFP5 (5¢-ACGCGTGGATCCATGGTG AGCAAGGGCGAG-3¢) and eGFP3 (5¢-CTCGAGAGATCTTTACTTGTAC AGCTCGTC-3¢) with pIRES2 (internal ribosomal entry site)-EGFP (Clontech, Palo Alto, CA, USA) as a template. The amplified eGFP sequence was initially cloned into pGem T easy (Promega, Madison, WI, USA), resulting in pGem T easy-eGFP.…”

Section: Construction Of Retroviral Vectorsmentioning

confidence: 99%

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A retroviral vector suitable for ultrasound image-guided gene delivery to mouse brain

et al. 2011

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Gene transfer to the early-stage embryonic brain using the ultrasound image-guided gene delivery (UIGD) technique has proven to be valuable for investigating brain development. Thus far, this technology has been restricted to the study of embryonic neurogenesis. When this technique is designed to be employed for the study in adult animals, a long-term stable gene expression will be required. We attempted to develop a retroviral vector suitable for expressing exogenous genes in the brains of postnatal and adult mice in the context of the UIGD technique. Retroviral vectors containing four different long terminal repeats (LTRs) (each from Moloney murine leukemia virus (MoMLV), murine stem cell virus (MSCV), myeloproliferative sarcoma virus (MPSV) and spleen focus-forming virus (SFFV)) were compared using the well-known CE vector having the EF1a internal promoter as a control. The MS vector containing MSCV LTR produced a higher viral titer and a higher level of gene expression than other vectors including CE. The MS vector drove the gene expression in cultured neural stem cells for 3 weeks. Furthermore, the MS vector could efficiently deliver the gene to the mouse central nervous system, as transgene expression was found in various regions of the brains and spinal cords as well as in all major neural cell types. The data from an in vivo luciferase imaging analysis showed that the gene expression from the MS vector was sustainable for almost 3 months. Our data suggested that the MS vector would be suitable to construct mice containing the transgene expressed in the brain or spinal cord in a quick and cost-effective manner.

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“…17 TCR a and b genes were amplified by reverse transcription-PCR and identified as TRAV8-1 and TRBV7-9, respectively. 18 The individual TCR genes were cloned into retroviral vector pMSP, 18,33 harboring a murine stem cell virus long terminal repeat and a murine phosphoglycerate kinase promoter, to produce MS-bPa, in which murine stem cell virus long terminal repeat drives b gene and phosphoglycerate kinase promoter drives a gene. PG13 producer clones were generated and screened to produce high titer virus (Figure 2a).…”

Section: Generation Of Gd T Cellsmentioning

confidence: 99%

Rapid αβ TCR-mediated responses in γδ T cells transduced with cancer-specific TCR genes

et al. 2009

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Adoptive T-cell transfer of in vitro cultured T cells derived from cancer patients with naturally developed immune responses has met with some success as an immunotherapeutic approach, although only a limited number of patients showed spontaneous immune responses. To find alternative ways, such as cancer-specific T-cell receptor (TCR) gene transfer, in preparation for sufficient numbers of antigenspecific T cells is an important issue in the field of adoptive T-cell therapy. Given the inherent disadvantage of ab TCR transfer to other ab T cells, namely the possible formation of mixed TCR heterodimers with endogenous a or b TCR, we employed gd T cells as a target for retroviral transfer of cancer-specific TCR and examined whether gd T cells were useful as an alternative population for TCR transfer. Although retroviral transduction to gd T cells with TCR ab genes alone, isolated from a MAGE-A4 143-151 -specific ab CD8 + cytotoxic T lymphocyte (CTL) clone, did not provide sufficient affinity to recognize major histocompatibility (MHC)-peptide complexes due to the lack of CD8 co-receptor, gd T cells co-transduced with TCR ab and CD8 ab genes acquired cytotoxicity against tumor cells and produced cytokines in both ab-and gd-TCR-dependent manners. Furthermore, ab TCR and CD8-transduced gd T cells, stimulated either through ab TCR or gd TCR, rapidly responded to target cells compared with conventional ab T cells, reminiscent of gd T cells. We propose ab TCRtransduced gd T cells as an alternative strategy for adoptive T-cell transfer.

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Factors affecting the performance of different long terminal repeats in the retroviral vector

Cited by 10 publications

References 22 publications

Nonintegrating Foamy Virus Vectors

Nonintegrating Foamy Virus Vectors

A retroviral vector suitable for ultrasound image-guided gene delivery to mouse brain

Rapid αβ TCR-mediated responses in γδ T cells transduced with cancer-specific TCR genes

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