Factors Affecting the Staining with Colloidal Gold

Ultrastructural triple localization of laminin-1, nidogen-1, and collagen type IV helps elucidate basement membrane structure in vivo

“…During performance of triple localization, unknown interactions between the three colloidal gold probes occur. The prolonged protocol worsens the limitations already manifest in single immunogold‐histochemistry (Park et al, 1989). However, immunogold‐histochemistry is the only method yielding insight into the BM structure in vivo.…”

Preparation, use, and enlargement of ultrasmall gold particles in immunoelectron microscopy

“…The protective power of a protein, i.e., the minimal amount of a protein (''gold number'') needed to stabilize a colloid against electrolyte-induced aggregation indicated by a red-blue shift of the gold sol, remains the main criterion for quantifying the protein-gold interaction. This minimal amount of protein varies for different proteins (e.g., Park et al, 1989), and was also the base for the temporary use of gold colloids for comparative protein titration in medical diagnostics (Gmelin, 1954).…”

Controlling deposition of nanoparticles by tuning surface charge of SiO2by surface modifications

“…In order to compare our simulations and experimental results, we must calibrate the number of iterations in the ERSA model with the total number of particles that will attempt to deposit on the substrate during a specific time interval, which in turn depends on the diffusion rate of the particles. The number of particles, N , that approach the substrate of a specific area within a given time is given by 43–45 . Here the area in which the model simulates the particles is denoted by W , the nanoparticle concentration by C 0 (3.8 × 10 11 particles per mL), the viscosity of the solvent by η (8.9 mg cm –1 s –1 ), and the duration of the deposition by t (60 min).…”