Many factors such as environment, herbicide rate, growth stage at application and days between sequential applications can influence response of a crop to herbicides. Florpyrauxifen-benzyl is a new broad-spectrum, POST herbicide commercialized in U.S. rice in 2018. Field experiments were conducted in 2018 at the Pine Tree Research Station (PTRS) near Colt, AR, and the Rice Research and Extension Center (RREC), near Stuttgart, AR, evaluating crop injury and yield response of three rice cultivars to sequential applications of florpyrauxifen-benzyl. Greenhouse and growth chamber experiments were conducted at the Altheimer Laboratory in Fayetteville, AR, evaluating cultivar responses when florpyrauxifen-benzyl was applied at 30 or 60 g ae ha -1 to rice exposed to different temperature regimes or at various growth stages. Three rice cultivars were used in all experiments: long-grain variety ‘CL111’, medium-grain variety ‘CL272’, and long-grain hybrid cultivar ‘CLXL745’. CL111 exhibited sufficient tolerance to florpyrauxifen-benzyl with only 10% visible injury and no effect on yield. CL272 showed 15% injury 3 wks after the second application in the field experiment when applications were made 14 d apart. Additionally, 12% injury was observed in the greenhouse when florpyrauxifen-benzyl was applied at 30 g ae ha -1, averaged over growth stages at application. Florpyrauxifen-benzyl did not reduce the yield of CL272 in the field, indicating CL272 can recover from florpyrauxifen-benzyl injury. As much as 64% injury was observed for CLXL745 at 3 weeks after application (WAA) when sequential herbicide applications were made 4 d apart. High levels of injury occurred in the growth chamber and greenhouse studies for this cultivar as well. Sequential applications of florpyrauxifen-benzyl reduced yields of CLXL745 in nearly all treatments. Data from these experiments suggest CL272 and CLXL745 are sensitive to sequential applications of florpyrauxifen-benzyl. Growers must follow the prescribed guidelines for using florpyrauxifen-benzyl in these cultivars and others like it.